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Anti human ifn γ antibody

Manufactured by BioLegend
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Sourced in United States

Anti-human IFN-γ antibody is a laboratory reagent used to detect and quantify human interferon-gamma (IFN-γ) in various biological samples. It is a specific antibody that binds to IFN-γ, allowing researchers to measure its presence and concentration.

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Lab products found in correlation

Amicon ultra 0.5 ml centrifugal filter, by Merck Group (1 mentions) Spectramax 190, by Molecular Devices (1 mentions) Human il 7, by Thermo Fisher Scientific (1 mentions) Mouse il 7, by Thermo Fisher Scientific (1 mentions) Avidin hrp, by BioLegend (1 mentions) Immobilon p membrane, by Merck Group (1 mentions)

Close spelling variants of this product

FITC anti-human IFN-γ antibody PE anti-human IFN-γ Antibody Ultra-LEAF anti-human IFN-γ antibody PE/Cyanine7 anti-human IFN-γ antibody Anti-IFN-γ antibody Human IFN-γ Anti-IFN-γ IFN-γ

4 protocols using anti human ifn γ antibody

1

IFN-γ ELISpot Assay Protocol

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Plates with Immobilon-P membrane (Millipore) were activated and coated with anti-human IFNγ antibody (Clone NIB42, Biolegend). After blocking with 1% BSA, 5×105 PBMCs or 2×105 T cells were co-incubated with respective peptides, antigens, or stimulants. After 24 hours, the plate was washed and incubated with anti-human IFNγ antibody (Clone 4S.B3, Biolegend). After washing, the plate was incubated with avidin-HRP (Biolegend), developed using the BD Elispot AEC Substrate and analyzed with ImmunoSpot-Reader systems. All antibodies were used according to the manufacturers’ recommendation.
Grauwet K., Berger T., Kann M.C., Silva H., Larson R., Leick M.B., Bailey S.R., Bouffard A.A., Millar D., Gallagher K., Turtle C.J., Frigault M.J, & Maus M.V. (2024). Stealth transgenes enable CAR-T cells to evade host immune responses. Journal for Immunotherapy of Cancer, 12(5), e008417.
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2

Concentrating Anti-Human IFN-γ Antibody

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The purified anti-human IFN-γ antibody (BioLegend, Cat#502502, San Diego, CA) was concentrated using 100 K MWCO Amicon Ultra 0.5 mL Centrifugal filters [Millipore, Cat#UFC510024, Billerica, MA] according to the user’s manual to achieve a final concentration of ~2–3 mg/mL. Briefly, the antibody was washed with 500 μL PBS (100 mM sodium phosphate, 150 mM NaCl, pH = 7.4) by centrifuging the unit at 14 000 × g for 5 min for a total of four times. The absorbance of each wash was measured using UV–vis spectroscopy at 280 nm (Spectramax 190, Molecular Devices, Sunnyvale, CA) to confirm that there was no loss of the antibody. After collecting the concentrated purified anti-human IFN-γ antibody, the final concentration of the antibody was measured with UV–vis spectroscopy at 280 nm.
Moutsiopoulou A., Hunt E., Broyles D., Pereira C.A., Woodward K., Dikici E., Kaifer A., Daunert S, & Deo S.K. (2017). Bioorthogonal Protein Conjugation: Application to the Development of a Highly Sensitive Bioluminescent Immunoassay for the Detection of Interferon-γ. Bioconjugate chemistry, 28(6), 1749-1757.
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3

Neutralizing CIK Cell Cytokines

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Anti-human IFN-γ antibody, anti-human TNF-α antibody, or anti-human IL-2 antibody (1µg/mL; all purchased from Biolegend, USA) was added to the co-culture system of A549/DDP and CIK cells to neutralize these cytokines secreted by CIK cells. A549, A549/DDP cultured alone and A549/DDP co-cultured with CIK cells without the neutralizing antibody treatment were set as the control groups. The DDP resistance of these cells and the expression levels of GST-π were detected.
Yang L., Du C., Wu L., Yu J., An X., Yu W., Cao S., Li H, & Ren X. (2017). Cytokine-Induced Killer Cells Modulates Resistance to Cisplatin in the A549/DDP Cell Line. Journal of Cancer, 8(16), 3287-3295.
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4

Notch Signaling Activation in Mouse and Human T Cells

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Mouse and human T cells were activated using the methods mentioned above. To activate Notch signalling, activated T cells were cocultured with Notch ligand-expressing OP9 feeder cells, OP9-DL1 cells for mouse T cells and OP9-hDL1 cells for human T cells. We cultured mouse T cells and OP9-DL1 cells with mouse IL-7 (PeproTech; 10 ng ml−1), an anti-mouse IFN-γ antibody (R4-6A2; 2 μg ml−1), in alpha MEM for 11–12 days. Human T cells and OP9-hDL1 cells were cocultured with human IL-7 (PeproTech; 10 ng ml−1), an anti-human IFN-γ antibody (Bio Legend, B27; 2 μg ml−1), in alpha MEM for 11 days.
Kondo T., Morita R., Okuzono Y., Nakatsukasa H., Sekiya T., Chikuma S., Shichita T., Kanamori M., Kubo M., Koga K., Miyazaki T., Kassai Y, & Yoshimura A. (2017). Notch-mediated conversion of activated T cells into stem cell memory-like T cells for adoptive immunotherapy. Nature Communications, 8, 15338.
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