Cells were transfected with 20 nM siRNA targeting RIOK3 (SI02223396; siRIOK3) or AllStars Negative control (1027281; siCon) using HiPerFect reagent (Qiagen, Manchester, UK). Custom siRNA oligonucleotides containing 3 sequences targeting HIF-1α (1 CAAGCAACTGTCATATATA, 2 TGCCACCACTGATGAATTA, 3 TGACTCAGCTATTCACCAA) or HIF-2α (1 TAACGACCTGAAGATTGAA, 2 CAAGCCACTGAGCGCAAAT, 3 TGAATTCTACCATGCGCTA) were purchased from Eurogentec (Southampton, UK). pGIPZ plasmids targeting RIOK3 (Clone ID V2LHS_16875; shRIOK3) or non-silencing control shRNAmir (RHS4743; shCon) were obtained from Thermo Scientific. Lentivirus packaging and titration was carried out as described.38 (link) Cells were transduced at MOI 1 and pools of transduced cells were selected with 1 μg/mL puromycin (Life Technologies) 48 h post-transduction.
Custom sirna oligonucleotides
Manufactured by Eurogentec
Sourced in United Kingdom
Custom siRNA oligonucleotides are short, double-stranded RNA molecules designed to target and silence specific genes. They are used in research to study gene function and can be tailored to the specific needs of the user.
Automatically generated - may contain errors
Lab products found in correlation
Hiperfect reagent, by Qiagen (2 mentions)
Puromycin, by Thermo Fisher Scientific (2 mentions)
Si02223396, by Qiagen (2 mentions)
Siriok3, by Qiagen (2 mentions)
Clone id v2lhs 16875 shriok3, by Thermo Fisher Scientific (2 mentions)
Pgipz plasmids, by Thermo Fisher Scientific (2 mentions)
Rhs4743, by Thermo Fisher Scientific (2 mentions)
2 protocols using custom sirna oligonucleotides
1
Silencing RIOK3 and HIF Transcription
2
Silencing RIOK3 and HIF Transcription
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Cells were transfected with 20 nM siRNA targeting RIOK3 (SI02223396; siRIOK3) or AllStars Negative control (1027281; siCon) using HiPerFect reagent (Qiagen, Manchester, UK). Custom siRNA oligonucleotides containing 3 sequences targeting HIF-1α (1 CAAGCAACTGTCATATATA, 2 TGCCACCACTGATGAATTA, 3 TGACTCAGCTATTCACCAA) or HIF-2α (1 TAACGACCTGAAGATTGAA, 2 CAAGCCACTGAGCGCAAAT, 3 TGAATTCTACCATGCGCTA) were purchased from Eurogentec (Southampton, UK). pGIPZ plasmids targeting RIOK3 (Clone ID V2LHS_16875; shRIOK3) or non-silencing control shRNAmir (RHS4743; shCon) were obtained from Thermo Scientific. Lentivirus packaging and titration was carried out as described.38 (link) Cells were transduced at MOI 1 and pools of transduced cells were selected with 1 μg/mL puromycin (Life Technologies) 48 h post-transduction.
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