Maxwell rsc 48 instrument

Manufactured by Promega

Sourced in United States, United Kingdom

The Maxwell® RSC 48 Instrument is an automated nucleic acid extraction system designed to purify DNA, RNA, and viral nucleic acids from a variety of sample types. The instrument utilizes magnetic particle technology to efficiently capture and purify target analytes.

Automatically generated - may contain errors

Lab products found in correlation

Maxwell rsc plant rna kit, by Promega (5 mentions) Quant it dsdna high sensitivity kit, by Thermo Fisher Scientific (3 mentions) Maxwell rsc viral total nucleic acid purification kit, by Promega (3 mentions) Maxwell rsc whole blood dna kit, by Promega (3 mentions) Maxwell rsc cultured cells dna kit, by Promega (2 mentions) Genesig easy rna internal extraction control, by Primerdesign (2 mentions) High capacity cdna reverse transcription kit, by Thermo Fisher Scientific (2 mentions) Powerup sybr green master mix, by Thermo Fisher Scientific (2 mentions) Maxwell rsc dna ffpe kit, by Promega (2 mentions) Nanodrop spectrophotometer, by Thermo Fisher Scientific (2 mentions) Random primer, by Promega (2 mentions) Maxwell viral total nucleic acid purification kit, by Promega (2 mentions) Maxwell rsc simplyrna tissue kit, by Promega (1 mentions) Maxwell rsc blood dna kit, by Promega (1 mentions) Infinium omni2.5 8 v 1 3 beadchip, by Illumina (1 mentions) Maxwell rsc mirna plasma and serum kit, by Promega (1 mentions) Quantitect probe rt pcr kit, by Qiagen (1 mentions) Isogen ls, by Nippon Gene (1 mentions) Ion reporter software, by Thermo Fisher Scientific (1 mentions) Maxwell rsc rna ffpe kit, by Promega (1 mentions)

32 protocols using maxwell rsc 48 instrument

FFPE Tumor Profiling using NGS Panels

Check if the same lab product or an alternative is used in the 5 most similar protocols

For panel sequencing, tumor-enriched areas were macrodissected from FFPE tissue sections.
DNA was isolated using the Maxwell RSC DNA FFPE Kit (Promega, Madison, Wisconsin, USA) on a Maxwell RSC 48 Instrument (Promega) and analyzed using the nNGM panel v1 (ThermoFisher, Waltham, Massachusetts, USA), an Ion 530 chip (ThermoFisher) and the Ion Chef/Ion S5 XL System (ThermoFisher).
RNA was isolated using the Maxwell RSC RNA FFPE Kit (Promega) on a Maxwell RSC 48 Instrument (Promega) and analyzed using the Oncomine Focus RNA Assay (ThermoFisher), an Ion 530 chip and the Ion Chef/Ion S5 XL System (ThermoFisher).
The Sequence Pilot Software (Version 4.4.0, JSI Medical Systems) and the Ion Reporter Software (Version 5.12, ThermoFisher) were used for variant calling.

Bischoff P., Trinks A., Obermayer B., Pett J.P., Wiederspahn J., Uhlitz F., Liang X., Lehmann A., Jurmeister P., Elsner A., Dziodzio T., Rückert J.C., Neudecker J., Falk C., Beule D., Sers C., Morkel M., Horst D., Blüthgen N, & Klauschen F. (2021). Single-cell RNA sequencing reveals distinct tumor microenvironmental patterns in lung adenocarcinoma. Oncogene, 40(50), 6748-6758.

+ Open protocol

+ Expand

Quantifying Viral Shedding in Avian Influenza

Check if the same lab product or an alternative is used in the 5 most similar protocols

To assess viral shedding, the OP and CL swabs collected at 1, 3, 5, 7, 10, and 14 dpi were suspended in 1 mL PBS. Viral titers in the tissues from H9N2 AIV-infected birds were measured using the centrifuged tissue homogenates (10%, w/v). Briefly, 200 μL of the suspension (supernatant) was subjected to RNA extraction using the Maxwell RSC simplyRNA Tissue kit and a Maxwell RSC 48 instrument (Promega, Germany). The cycle threshold (Ct) value was used to calculate the viral load after real-time reverse transcriptase-PCR (rRT-PCR) of the M-gene (Spackman et al., 2003 (link)).
To convert the Ct values to infectious units, quantitative viral standards (ranging from 10^6.0 to 10^0.0 EID₅₀/0.1 mL) of each virus were prepared in egg allantoic fluid. Viral RNA was extracted from these standards and quantified by rRT-PCR. The resulting standard curves showed a high correlation (r² > 0.99) and were used to convert Ct values to EID₅₀ equivalents/0.1 mL. The detection limit for each virus was 10^1.0 EID₅₀/0.1 mL, with Ct values of 39 for LBM261/20 and 38 for LBM284/18 (Supplementary Figure 1). If the Ct value was >35, swabs were retested by isolating virus as described below.

Kye S.J., Park M.J., Kim N.Y., Lee Y.N., Heo G.B., Baek Y.K., Shin J.I., Lee M.H, & Lee Y.J. (2021). Pathogenicity of H9N2 low pathogenic avian influenza viruses of different lineages isolated from live bird markets tested in three animal models: SPF chickens, Korean native chickens, and ducks. Poultry Science, 100(9), 101318.

+ Open protocol

+ Expand

Saliva-based RNA Extraction Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Saliva samples in 5 ml polypropylene tubes packed in double plastic bags were first heat inactivated at 90 °C for 15 min in a dry oven. Following inactivation, pooling samples were created by combining four 75 μl aliquots of individual saliva samples into one 300 μl sample, and either pooled or individual samples were processed according to the instructions of isolation RNA using the Maxwell RSC Viral Total Nucleic Acid Purification Kit with the Maxwell RSC 48 Instrument (Promega, Madison, WI, USA). Briefly, 300 μl saliva was mixed with 330 µl lysis buffer containing proteinase K, incubated at 56 °C for 10 min and briefly centrifuged. Next, each sample was applied to separate wells of disposable cartridges in an RNA Maxwell RSC 48 Instrument for semi-automatic RNA extraction.

Różański M., Walczak-Drzewiecka A., Witaszewska J., Wójcik E., Guziński A., Zimoń B., Matusiak R., Kazimierczak J., Borowiec M., Kania K., Paradowska E., Pawełczyk J., Dziadek J, & Dastych J. (2022). RT-qPCR-based tests for SARS-CoV-2 detection in pooled saliva samples for massive population screening to monitor epidemics. Scientific Reports, 12, 8082.

+ Open protocol

+ Expand

Validation of Genetic Variant Panel

Check if the same lab product or an alternative is used in the 5 most similar protocols

The DNA was extracted from saliva and whole blood specimens using the Maxwell^® RSC Blood DNA Kit (Promega, Madison, Wisconsin) on the automated Maxwell^® RSC 48 Instrument (Promega), as well as being sourced from the Coriell Cell Repository. The validation set included 157 samples: (1) DNA from: 1000 Genomes Project [8 (link)] (n = 3), Coriell (n = 105), and CAP proficiency samples (n = 2); and (2) previously molecularly characterized clinical saliva (n = 11) and whole blood samples (n = 15). The samples were selected based on the absence/presence of known pathogenic/likely pathogenic variants in the genes included on the panel, prior pharmacogenetic characterization for specific star allele and non-star allele genes, and the availability of the material for the clinical samples. The clinical samples were fully blinded for the purposes of this validation and were handled according to the Health Insurance Portability and Accountability Act standards, and the internal samples were derived from individuals who had consented to the use of their sample for de-identified research as a secondary purpose at the time of their original clinical test request. Table S1 describes all of the samples used in this study, with biorepository numbers, as well as previous variant characterizations and the rationale for inclusion.

Silver A., Lazarin G.A., Silver M., Miller M., Jansen M., Wechsberg C., Dekanek E., Grossfeld S., Herpel T., Gunatilake D., Bisignano A, & Jaremko M. (2022). Technical Performance of a 430-Gene Preventative Genomics Assay to Identify Multiple Variant Types Associated with Adult-Onset Monogenic Conditions, Susceptibility Loci, and Pharmacogenetic Insights. Journal of Personalized Medicine, 12(5), 667.

+ Open protocol

+ Expand

Karyotype Analysis for Cell Line Validation

Check if the same lab product or an alternative is used in the 5 most similar protocols

G-banded karyotype analysis was performed on 5 × 10⁶ cells collected at passage five (for HiFi, Nanopore and Omni-C) and passage eight (for ONT-UL). For all cell lines, 20 metaphase cells were counted, and a minimum of 5 metaphase cells were analysed and karyotyped. Chromosome analysis was performed at a resolution of 400 bands or greater. A pass/fail criterion was used before cell lines proceeded to sequencing. Cell lines with normal karyotypes (46,XX or 46,XY) or lines with benign polymorphisms that are frequently seen in apparently healthy individuals were classified as passes. Cell lines were classified as failures if two or more cells harboured the same chromosomal abnormality. DNA used for microarray was isolated from frozen cell pellets (3 × 10⁶ to 7 × 10⁶ cells) using a Maxwell RSC Cultured Cells DNA kit on a Maxwell RSC 48 instrument (Promega). DNA was genotyped at the Children’s Hospital of Philadelphia’s Center for Applied Genomics using an Infinium Omni2.5-8 v.1.3 BeadChip (Illumina) on an iScan System instrument (Illumina).

Liao W.W., Asri M., Ebler J., Doerr D., Haukness M., Hickey G., Lu S., Lucas J.K., Monlong J., Abel H.J., Buonaiuto S., Chang X.H., Cheng H., Chu J., Colonna V., Eizenga J.M., Feng X., Fischer C., Fulton R.S., Garg S., Groza C., Guarracino A., Harvey W.T., Heumos S., Howe K., Jain M., Lu T.Y., Markello C., Martin F.J., Mitchell M.W., Munson K.M., Mwaniki M.N., Novak A.M., Olsen H.E., Pesout T., Porubsky D., Prins P., Sibbesen J.A., Sirén J., Tomlinson C., Villani F., Vollger M.R., Antonacci-Fulton L.L., Baid G., Baker C.A., Belyaeva A., Billis K., Carroll A., Chang P.C., Cody S., Cook D.E., Cook-Deegan R.M., Cornejo O.E., Diekhans M., Ebert P., Fairley S., Fedrigo O., Felsenfeld A.L., Formenti G., Frankish A., Gao Y., Garrison N.A., Giron C.G., Green R.E., Haggerty L., Hoekzema K., Hourlier T., Ji H.P., Kenny E.E., Koenig B.A., Kolesnikov A., Korbel J.O., Kordosky J., Koren S., Lee H., Lewis A.P., Magalhães H., Marco-Sola S., Marijon P., McCartney A., McDaniel J., Mountcastle J., Nattestad M., Nurk S., Olson N.D., Popejoy A.B., Puiu D., Rautiainen M., Regier A.A., Rhie A., Sacco S., Sanders A.D., Schneider V.A., Schultz B.I., Shafin K., Smith M.W., Sofia H.J., Abou Tayoun A.N., Thibaud-Nissen F., Tricomi F.F., Wagner J., Walenz B., Wood J.M., Zimin A.V., Bourque G., Chaisson M.J., Flicek P., Phillippy A.M., Zook J.M., Eichler E.E., Haussler D., Wang T., Jarvis E.D., Miga K.H., Garrison E., Marschall T., Hall I.M., Li H, & Paten B. (2023). A draft human pangenome reference. Nature, 617(7960), 312-324.

+ Open protocol

+ Expand

DNA Collection and Extraction from Saliva

Check if the same lab product or an alternative is used in the 5 most similar protocols

All participants donate 2 ml of saliva for DNA. Saliva is collected into SDNA-1000 kits from Spectrum Solutions^™ (Draper, Utah, USA), which stabilize the DNA for shipping and storage at room temperature. Participants are instructed not to eat, drink, smoke, or chew gum for 30 minutes prior to collecting the sample. Participants recruited in person are provided a saliva kit for sampling during their appointment, while those recruited online are mailed a saliva kit with a prepaid return envelope.
DNA from 1 ml of saliva is extracted in an automated fashion using Maxwell^® RSC Stabilized Saliva DNA Kits and a Maxwell^® RSC 48 Instrument from Promega (Madison, Wisconsin, USA). The remaining saliva solution is frozen at −80 degrees C.

Crowley J.J., Cappi C., Ochoa-Panaifo M.E., Frederick R.M., Kook M., Wiese A.D., Rancourt D., Atkinson E.G., Giusti-Rodriguez P., Anderberg J.L., Abramowitz J.S., Adorno V.R., Aguirre C., Alves G.S., Alves G.S., Ancalade N., Espinosa A.A., Arnold P.D., Ayton D.M., Barbosa I.G., Castano L.M., Barrera C.N., Prieto M.B., Berardo M.C., Berrones D., Best J.R., Bigdeli T.B., Burton C.L., Callahan J.L., Carneiro M.C., Cepeda S.L., Chazelle E., Chire J.M., Munoz M.C., Quiroz P.C., Cobite J., Comer J.S., Costa D.L., Crosbie J., Cruz V.O., Dager G., Daza L.F., de la Rosa-Gómez A., del Río D., Delage F.Z., Dreher C.B., Fay L., Fazio T., Ferrão Y.A., Ferreira G.M., Figueroa E.G., Fontenelle L.F., Forero D.A., Fragoso D.T., Gadad B.S., Garrison S.R., González A., Gonzalez L.D., González M.A., Gonzalez-Barrios P., Goodman W., Guintivano J., Guttfreund D.G., Guzick A.G., Halvorsen M.W., Hovey J.D., Janssen-Aguilar R., Jensen M., Reynolds A.Z., Lujambio J.A., Khalfe N., Knutsen M.A., Lack C., Lanzagorta N., Lima M.O., Longhurst M.O., Martinez D.A., Luna E.S., Marques A.H., Martinez M., de Los Angeles Matos M., Maye C.E., McGuire J.F., Menezes G., Minaya C., Miño T., Mithani S.M., de Oca C.M., Morales-Rivero A., Moreira-de-Oliveira M.E., Morris O.J., Muñoz S.I., Naqqash Z., Bracho A.A., Bracho B.E., Rojas M.C., Castaman L.A., Ortega I., Patel D.I., Patrick A.K., Mino M.P., Orellana J.L., Stumpf B.P., Peregrina T., Duarte T.P., Piacsek K.L., Placencia M., Quarantini L.C., Quarantini-Alvim Y., Ramos R.T., Ramos I.C., Ramos V.R., Ramsey K.A., Ray E.V., Richter M.A., Riemann B.C., Rivas J.C., Rosario M.C., Ruggero C.J., Ruiz-Chow A.A., Ruiz-Velasco A., Sampaio A.S., Saraiva L.C., Schachar R.J., Schneider S.C., Schweissing E.J., Seligman L.D., Shavitt R.G., Soileau K.J., Stewart S.E., Storch S.B., Strouphauer E.R., Timpano K.R., Treviño-de la Garza B., Vargas-Medrano J., Vásquez M.I., Martinez G.V., Weinzimmer S.A., Yanez M.A., Zai G., Zapata-Restrepo L.M., Zappa L.M., Zepeda-Burgos R.M., Zoghbi A.W., Miguel E.C., Rodriguez C.I., Mallen M.C., Moya P.R., Borda T., Moyano M.B., Mattheisen M., Pereira S., Lázaro-Muñoz G., Martinez-Gonzalez K.G., Pato M.T., Nicolini H, & Storch E.A. (2023). Latin American Trans-ancestry INitiative for OCD genomics (LATINO): Study Protocol. medRxiv.

+ Open protocol

+ Expand

Subgenomic RNA Quantification by RT-PCR

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total RNA was extracted from 125 µL of nasal or lung wash using ISOGEN-LS (Nippon gene, Toyko, Japan) and purified using a Maxwell RSC 48 Instrument (Promega, Madison, WI) with a Maxwell RSC miRNA Plasma and Serum Kit (Promega). Quantification of subgenomic RNA was performed by real-time reverse transcription PCR (RT-PCR) using a QuantiTect Probe RT-PCR Kit (QIAGEN, Hilden, Germany) with primers and probes as previously described [39] (link). Real-time RT-PCR was performed using Mx3005P (Stratagene, La Jolla, CA, USA).

Hemmi T., Ainai A., Hashiguchi T., Tobiume M., Kanno T., Iwata-Yoshikawa N., Iida S., Sato Y., Miyamoto S., Ueno A., Sano K., Saito S., Shiwa-Sudo N., Nagata N., Tamura K., Suzuki R., Hasegawa H, & Suzuki T. (2022). Intranasal vaccination induced cross-protective secretory IgA antibodies against SARS-CoV-2 variants with reducing the potential risk of lung eosinophilic immunopathology. Vaccine, 40(41), 5892-5903.

+ Open protocol

+ Expand

DNA Isolation from Frozen Tissues

Check if the same lab product or an alternative is used in the 5 most similar protocols

Total DNA from frozen tissues and cultured cells was isolated using the Maxwell^® RSC Cultured Cells DNA Purification Kit with a Maxwell^® RSC 48 instrument (Promega, Madison, WI, USA; AS1620). DNA purity and concentration were measured using a NanoDrop spectrophotometer (Thermo Fisher Scientific, Waltham, MA, USA).

Recalde M., Gárate-Rascón M., Herranz J.M., Elizalde M., Azkona M., Unfried J.P., Boix L., Reig M., Sangro B., Fernández-Barrena M.G., Fortes P., Ávila M.A., Berasain C, & Arechederra M. (2022). DNA Methylation Regulates a Set of Long Non-Coding RNAs Compromising Hepatic Identity during Hepatocarcinogenesis. Cancers, 14(9), 2048.

+ Open protocol

+ Expand

Maxwell RSC Viral RNA Extraction

Check if the same lab product or an alternative is used in the 5 most similar protocols

RNA was extracted using the Maxwell^Ⓡ RSC Viral Total Nucleic Acid Purification Kit (Promega UK Ltd., Southampton, UK) according to manufacturer's instructions. Briefly, a Class 1 microbiological safety cabinet (MSC) within a containment level 3 laboratory, 200 µl of sample was added to 223 µl of prepared lysis solution (including 5 µl per reaction of Genesig^Ⓡ Easy RNA Internal extraction control, Primerdesign Ltd, Chandler's Ford, UK). Samples were then inactivated for 10 min at room temperature within the MSC and 10 min at 56 °C on a heat block before automated RNA extraction using a Maxwell^Ⓡ RSC 48 Instrument (Promega UK Ltd., Southampton, UK). RNA was eluted in 50 µl of NFW. In the case of saliva, RNA was extracted from 200 µl of saliva diluted 1:20, as saliva volume was insufficient unless a dilution was performed.

Fowler V.L., Armson B., Gonzales J.L., Wise E.L., Howson E.L., Vincent-Mistiaen Z., Fouch S., Maltby C.J., Grippon S., Munro S., Jones L., Holmes T., Tillyer C., Elwell J., Sowood A., de Peyer O., Dixon S., Hatcher T., Patrick H., Laxman S., Walsh C., Andreou M., Morant N., Clark D., Moore N., Houghton R., Cortes N.J, & Kidd S.P. (2020). A highly effective reverse-transcription loop-mediated isothermal amplification (RT-LAMP) assay for the rapid detection of SARS-CoV-2 infection. The Journal of Infection, 82(1), 117-125.

+ Open protocol

+ Expand

Spelt Accession Transcriptional Analysis

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

A subset of 20 spelt accessions grown in a glasshouse illuminated with LX602C LED grow lights (Heliospectra, 20/4 hours day/night) were used for quantitative real-time PCR (RT-qPCR) studies. Spikelets from spikes 15 days after booting were collected in three biological replicates for RNA isolation. In the case of N. benthamiana, three leaf disks per A. tumefaciens infiltrated sample and three biological replicates per construct were collected for RNA isolation. RNA extraction was done using the Maxwell^® RSC Plant RNA kit using a Maxwell^® RSC 48 instrument (Promega). In all, 1 µg of RNA was used for the first-strand complementary DNA (cDNA) synthesis using the high-capacity cDNA reverse transcription kit following the user guidelines (Applied Biosystems catalog # 4368814). cDNA was further diluted 10 fold and 2 µl was used for RT-qPCR. RT-qPCR was performed for Rg1 and flavonoid biosynthetic genes using primers listed in Supplementary Data 5. A 10 µl RT-qPCR reaction was set-up and run on an ABI QuntStudio 6 Flex Real-Time PCR machine using PowerUp SYBR green master mix (Applied Biosystems catalog # AS25741). The 2^−ΔΔCT method was used to normalize and calibrate transcript values relative to endogenous controls Ta.6863 (spelt)^{83 (link)} and tubulin (NtTub1) (N. benthamiana).

Abrouk M., Athiyannan N., Müller T., Pailles Y., Stritt C., Roulin A.C., Chu C., Liu S., Morita T., Handa H., Poland J., Keller B, & Krattinger S.G. (2021). Population genomics and haplotype analysis in spelt and bread wheat identifies a gene regulating glume color. Communications Biology, 4, 375.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!