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Uh4150 uv visible spectrophotometer

Manufactured by Hitachi
Sourced in Japan

The UH4150 UV-Visible Spectrophotometer is a laboratory instrument designed to measure the absorbance or transmittance of light in the ultraviolet and visible regions of the electromagnetic spectrum. It is used to analyze and quantify the concentrations of various chemical compounds in a sample.

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4 protocols using uh4150 uv visible spectrophotometer

1

Quantifying Oxidative Stress via MDA Assay

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The MDA was analyzed using the Malondialdehyde (MDA) Content Assay Kit (Solarbio, Beijing, China) according to the manufacturer’s instructions. At the end of the reaction, the absorbance of each sample was measured at 450 nm, 532 nm and 600 nm using a UH4150 UV-Visible Spectrophotometer (Hitachi, Tokyo, Japan). The amount of MDA was calculated according to the manufacturer’ s instructions.
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2

Comprehensive Characterization of Nanomaterials

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Powder X-ray diffraction (PXRD) patterns of the samples were acquired on a Riguku D/Max-2500PC X-ray diffractometer with Cu radiation (λ = 1.54178 Å). Fourier transform infrared (FT-IR) spectroscopy was conducted using a Bruker ALPHA II FT-IR spectrometer. X-ray photoelectron spectroscopy (XPS) analysis was obtained on an ESCALAB 250 instrument operated at 150 W and 200 eV with mono chromated Al Kα radiation. Thermogravimetric analyses (TGA) were performed on an SDT 2960 thermal analyzer from room temperature (RT) to 800 °C at a heating rate of 10 °C/min under a nitrogen atmosphere. Dynamic light scattering (DLS) data were obtained by Horiba nano Partica SZ-100V2. UV–vis absorption spectra were recorded using a Hitachi UH4150 UV–visible spectrophotometer in the range of 200–700 nm. Emission and excitation spectra at RT were recorded with an Edinburgh FLS 1000 fluorescence spectrometer, and luminescence microscopy images were recorded on an Olympus BX53 microscope. Electron paramagnetic resonance (EPR) spectra were recorded by a Bruker A 300 EPR spectrometer.
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3

Structural Characterization of Cu3NC Complexes

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Powder X-ray diffraction (PXRD) patterns of Cu3NC(NHC), Cu3NC(BINAP), and Cu3NC(Pz) were recorded on a Rigaku B/Max-RB X-ray diffractometer with Cu-Kα radiation (λ = 1.5418 Å) in air at room temperature. ESI–MS images of the clusters were recorded on an AB Sciex X500R Q-TOF spectrometer. UV‒vis absorption spectra were obtained through a Hitachi UH4150 UV‒visible spectrophotometer. The X-ray diffraction data of Cu3NC(NHC) and Cu3NC(Pz) were measured on a Rigaku XtaLAB Pro diffractometer (Supplementary Tables 3 and 4).
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4

Quantifying Nitrite Levels in Cell Culture

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Different concentrations of NO2 standard solutions were prepared using NaNO2 (analytical pure) and ddH2O. Firstly, adding 5 mL of cell-free medium was to a Petri dish, after CAP treatment, the media was collected, 100 µL of hydrochloric acid (1 M) and 100 µL of Sulphanilamide (10 g/L) were added and the reaction was carried out at room temperature for 5 min. After this reaction, 100 μL of N-(1-naphthy1)-ethylenediamine hydrochloride (1 g/L) was added and the reaction was carried out at room temperature for another 1 h. At the end of the reaction, 2 mL of the mixture was added to a quartz cuvette and the absorbance of the standard solution and the sample was measured at 540 nm using a UH4150 UV-Visible Spectrophotometer (Hitachi, Tokyo, Japan). A standard curve was plotted according to the absorbance of the standard solution, and the content of NO2 in the treated medium was calculated.
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