Meso quickplex sq 120 imager

Manufactured by MSD

Sourced in United States

The MESO QuickPlex SQ 120 imager is a multiplex immunoassay system designed for the quantitative analysis of multiple analytes in a single sample. It utilizes electrochemiluminescence technology to detect and measure target proteins or other molecules. The device offers automated sample processing and analysis, providing researchers with efficient and reliable data generation.

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Lab products found in correlation

V plex plus aβ peptide panel 1 kit, by MSD (1 mentions) Msd discovery workbench software, by Mesoscale (1 mentions)

5 protocols using meso quickplex sq 120 imager

Multiplex Cytokine Quantification by HPE

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Plasma cytokine concentrations were measured by high-performance electrochemiluminiscence (HPE) using the multiplex 1 V-PLEX Human Proinflammatory (IL-10, IL-6, TNFα, and IL-1β) and Human Chemokine (IP-10) panels. The multiplex arrays were analyzed with a MESO QuickPlex SQ 120 imager (MSD, Rockville, MD) and Discovery Workbench v4.0 software. Concentrations were obtained in duplicate per each sample in accordance with the manufacturer’s protocol. The following reflect the published manufacturer standards for the lower limit of detectability (LoD) and the lower to upper limit of quantification (LoQ) for each of the measured proteins: IL-6: LLoD 0.06pg/mL and LoQ 1.58–488pg/mL; TNFα: LLoD 0.04pg/mL and LoQ 0.69–248pg/mL; IL-10: LLoD 0.03pg/mL and 0.68–233; IP-10: LLoD 0.37pg/mL and LoQ 1.37–500pg/mL; IL-1β: LLoD 0.04pg/mL and LoQ 2.14–375 pg/mL.

Casaletto K.B., Elahi F.E., Fitch R., Walters S., Fox E., Staffaroni A.M., Bettcher B., Zetterberg H., Karydas A., Rojas J.C., Boxer A.L, & Kramer J.H. (2018). A Comparison of Biofluid Cytokine Markers across Platform Technologies: Correspondence or Divergence?. Cytokine, 111, 481-489.

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Quantifying Aβ Peptide Secretion

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Secretion of Aβ peptides; Aβ38, Aβ40, and Aβ42, was assessed using the V-PLEX Plus Aβ Peptide Panel 1 Kit (MSD, 6E10), and the MESO QUICKPLEX SQ 120 Imager (MSD), connected to the DISCOVERY WORKBENCH 4.0 software, according to the manufacture’s protocol. The data was normalized to the RNA concentration of the individual samples, and data was obtained from three independent experiments.

Haukedal H., Corsi G.I., Gadekar V.P., Doncheva N.T., Kedia S., de Haan N., Chandrasekaran A., Jensen P., Schiønning P., Vallin S., Marlet F.R., Poon A., Pires C., Agha F.K., Wandall H.H., Cirera S., Simonsen A.H., Nielsen T.T., Nielsen J.E., Hyttel P., Muddashetty R., Aldana B.I., Gorodkin J., Nair D., Meyer M., Larsen M.R, & Freude K. (2023). Golgi fragmentation – One of the earliest organelle phenotypes in Alzheimer’s disease neurons. Frontiers in Neuroscience, 17, 1120086.

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Quantification of Alzheimer's Biomarkers

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Aβ40 and Aβ42 were measured using the V-PLEX Aβ peptide panel 1 (6E10) assay (MSD (Meso Scale Discovery), catalog no. K15200E). sAPPα and sAPPβ were measured using the sAPPα/sAPPβ multiplex assay kit (MSD, catalog no. K15120E) according to the manufacturer's instructions. Assay plates were blocked, and conditioned cell medium samples and standards buffered with 500 mm HEPES, pH 7.4, to a final concentration of 50 mm were loaded in duplicate. Following washing and secondary antibody incubation, assays were read using the MESO QUICKPLEX SQ 120 imager and analyzed using MSD Workbench 4.0 software. The protein concentration of the conditioned medium was determined by a bicinchoninic acid assay, and sAPPα, sAPPβ, and Aβ levels were corrected for total protein concentration.

Jarosz-Griffiths H.H., Corbett N.J., Rowland H.A., Fisher K., Jones A.C., Baron J., Howell G.J., Cowley S.A., Chintawar S., Cader M.Z., Kellett K.A, & Hooper N.M. (2019). Proteolytic shedding of the prion protein via activation of metallopeptidase ADAM10 reduces cellular binding and toxicity of amyloid-β oligomers. The Journal of Biological Chemistry, 294(17), 7085-7097.

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Multiplexed Immunoassay Protocol

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All chemicals were purchased from Merck (Kenilworth, NJ, USA) and Sigma-Aldrich (St. Louis, MO, USA). MSD GOLD 96well Streptavidin SECTOR Plates, MSD Read Buffer T (4x), SULFO-TAG labeled goat anti-mouse IgG and QuickPlex SQ 120 system containing the MESO QuickPlex SQ 120 Imager and MSD DISCOVERY WORKBENCH software were acquired from Meso Scale Diagnostics, LLC (Rockville, MD, USA).

Tzara O., Amalie Simonsen S., Sode West A., Asser Karsdal M., Klingenberg Iversen H, & Henriksen K. (2022). Quantification of Tau-A in serum after brain injury: a comparison of two analytical platforms, ELISA and electrochemiluminescence immunoassay. Brain injury, 36(6).

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Quantifying Intratumoral Cytokine Levels

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Quantitation of intratumoral protein levels of IL15, IL33, macrophage inflammatory protein 1 beta (MIP-1b), macrophage inflammatory protein 3 alpha (MIP-3a), and Monocyte Chemoattractant Protein 1 (MCP-1/CCL2) were performed using a Meso Scale Discovery (MSD) U-PLEX Biomarker Group 1 (Mouse) assay plate which utilized a linker technology and biotinylated capture antibodies (MSD). Cytokines were then detected using SULFO-TAG-labeled antibodies to generate the electrochemiluminescence readout and read using the MESO QuickPlex SQ 120 imager (MSD).

Haas M.S., Kagey M.H., Heath H., Schuerpf F., Rottman J.B, & Newman W. (2021). mDKN-01, a Novel Anti-DKK1 mAb, Enhances Innate Immune Responses in the Tumor Microenvironment. Molecular cancer research : MCR, 19(4).

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