Phenolsulfonphthalein (PSP)
was obtained from Macklin (Shanghai, China). Estradiol-17β-glucuronide
(E17βG), estrone-3-sulfate (E3S), and taurocholate (TCA) were
purchased from Sigma-Aldrich (St. Louis, MO). 4-(4-(Dimethylamino)styryl)-N-methylpyridinium (ASP+) and penciclovir (PCV)
were from US Everbright (Suzhou, China) and Aladdin (Shanghai, China),
respectively. Dulbecco’s modified Eagle’s medium (DMEM),
PSP-free DMEM, fetal bovine serum (FBS), trypsin, Lipofectamine 2000,
sulfo-N-hydroxysuccinimide-SS-biotin,
protease inhibitor tablets, and streptavidin-agarose resin were purchased
from Thermo Fisher Scientific (Waltham, MA). Penicillin-streptomycin
was from Hyclone (Logan, UT). The bicinchoninic acid (BCA) protein
assay kit was purchased from Takara (Kyoto, Japan). The first antibodies
for detecting 6-His tag and Na+/K+-ATPase were
from Proteintech (Rosemont, IL) and Abcam (Boston, MA), respectively.
Their secondary antibodies were from Proteintech (Rosemont, IL).
was obtained from Macklin (Shanghai, China). Estradiol-17β-glucuronide
(E17βG), estrone-3-sulfate (E3S), and taurocholate (TCA) were
purchased from Sigma-Aldrich (St. Louis, MO). 4-(4-(Dimethylamino)styryl)-N-methylpyridinium (ASP+) and penciclovir (PCV)
were from US Everbright (Suzhou, China) and Aladdin (Shanghai, China),
respectively. Dulbecco’s modified Eagle’s medium (DMEM),
PSP-free DMEM, fetal bovine serum (FBS), trypsin, Lipofectamine 2000,
sulfo-N-hydroxysuccinimide-SS-biotin,
protease inhibitor tablets, and streptavidin-agarose resin were purchased
from Thermo Fisher Scientific (Waltham, MA). Penicillin-streptomycin
was from Hyclone (Logan, UT). The bicinchoninic acid (BCA) protein
assay kit was purchased from Takara (Kyoto, Japan). The first antibodies
for detecting 6-His tag and Na+/K+-ATPase were
from Proteintech (Rosemont, IL) and Abcam (Boston, MA), respectively.
Their secondary antibodies were from Proteintech (Rosemont, IL).