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9 protocols using hexane

1

Fabrication of Serotonin Aptamer Biosensor

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3,4-Ethylenedioxythiophene (EDOT), polyacrylonitrile (PAN), dimethylformamide (DMF), ferric chloride, 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM), hexamethyldisilazane (HMDS), 3,4-Dimethoxythiophene, p-toluenesulfonic acid monohydrate (pTsOH·H2O), butylated hydroxytoluene (BHT), tetrahydrofuran (THF), and Sylgard 184 polydimethylsiloxane (PDMS) were obtained from Sigma–Aldrich. Ethanol, toluene, mEthanol, hexane (Hex), hydrochloric acid (HCl), and ethyl acetate (EtOAc) were obtained from Samchun. Sodium hydroxide (NaOH) was purchased from Daejung. (S)-Methyl 2,3-dihydroxypropanoate (Methyl glycerate) was purchased from Combi-Block. AZ5214E was obtained from Clariant. A serotonin aptamer with the sequence 5′-CGACTGGTAGGCAGAT AGGGGAAGCTGATTCGAGCGTGGGTCG[C6 Amine]-3′ was synthesized by Bioneer. Phosphate buffered saline (PBS) pH 7.4 (1 ×) was purchased from Gibco™, and simulated blood serum and artificial CSF were purchased from Biochemazone. All reagents and solvents were used as received without further treatment.
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2

Synthesis of Conjugated Polymers

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Sodium, FeCl3, 2-methyl-2-butanol, 2-ethyl hexyl bromide, Pd(PPh3)4, CuI, (i-Pr)2NH, ethynyltrimethylsilane, N-bromosuccinimide, 3,4-ethylenedioxythiophene, and 2,5-dibromothiophene were purchased from Sigma-Aldrich. DMF and 2-carbonitrile thiophene were purchased from Alfa Aesar Co. Tetrahydrofuran, dichloromethane, toluene, methanol, ethyl acetate, hexane, CHCl3, and potassium carbonate were purchased from Samchun Pure Chemicals. Tetrahydrofuran (THF), diethyl ether, toluene, and methylene chloride were used after distillation in the presence of Sodium/benzophenone or calcium hydride under nitrogen gas.
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3

Microalgae Extraction and Antioxidant Assay

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Tetraselmis suecica was purchased from Chloland (Geoje-si, Gyeongsangnam-do, Korea). Hexane, heptane, EA, Ace, EtOH, MeOH, acetonitrile and sodium acetate were purchased from Samchun Chemical (Kangnam-gu, Seoul, Korea). Lutein, 2,2′-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), 2,4,6 tripyridyl-S-triazine (TPTZ), Iron(III) chloride (FeCl3) and Iron(II) sulfate (FeSO4) were purchased from Sigma-Aldrich (St. Louis, MO, USA). All reagents and chemicals in this study were used above analytical grade.
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4

Enzymatic Synthesis of Cellulose Esters

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Microcrystalline cellulose (MCC), 1-ethyl-3-methylimidazolium acetate ([Emim][Ac]), 40% tetrabutylammonium hydroxide (TBAH), 40% tetrabutylphosphonium hydroxide (TBPH), thiourea, Span® 85, iron oxide (Fe2O3), lipase from Candida rugosa (Type VII, 1176 U/mg), p-nitrophenyl butylate, and p-nitrophenol were purchased from Sigma Aldrich (St. Louis, MO, USA). Sodium hydroxide, ethanol, hexane, isopropyl alcohol, Congo red, and water (HPLC grade) were obtained from Samchun Pure Chemical (Gyeonggi-do, Korea). Soybean oil was purchase from SAJO Daerim (Incheon, Korea). All other chemicals used were of analytical grade and were used without further purification.
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5

Synthesis of Iron-based Nanomaterials

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Iron(ii) chloride (FeCl2·4H2O) (99%), iron(iii) chloride hexahydrate (FeCl3·6H2O) (99%), acetic acid (99.8%), hexane (99.5%), diethyl ether (99.5%), and ethanol (99.9%) were obtained from Samchun Chemical Co., Ltd. (Seoul, Republic of Korea). Ammonia solution (28%), tetraethyl orthosilicate (TEOS) (98%), (3-aminopropyl)triethoxysilane (APTES) (≥98%), and trichloroacetic acid (TCA) (99%) were obtained from Sigma Aldrich (Munich, Germany).
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6

Graphite-Coated Sponge for Oil Absorption

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The base PU sponge (25
kg/m3) was obtained from Total Sponge Co., Ltd. Graphite
powder has a size of 20 μm or less as a coating material, and
the products of Sigma-Aldrich Chemistry Co., Ltd. were used. Ethanol
(99.0%, C2H5OH) for dispersing graphite was
purchased from Samchun Pure Chemical Co., Ltd. Dow Corning Sylgard
184 base and curing agent product was used as the curing agent for
curing PDMS and PDMS with another coating material. Toluene (99.9%,
C7H8) used for diluting PDMS was a product of
SK Chemicals. For performance testing, we used gasoline, a product
of SK energy, soybean oil, xylene (99.0%, C8H10), a Sigma-Aldrich Co., Ltd. product, and hexane (99.9%, C6H6) and chloroform (99.5%, CHCl3) from Samchun
Pure Chemical Co., Ltd. For silicone oil, KF-96 from Shin-Etsu Chemical
Co., Ltd. was used. Lastly, oil red O (dye content ≥ 75%, C26H24N4O) used for dyeing organic solvents
was purchased from Sigma-Aldrich. Co., Ltd.
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7

Cholesterol Extraction from Egg Yolks

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Eggs were purchased from a retail store with the average size of 62.67±0.25 g and powdered crosslinked β-CD was obtained from MSC Co. (Yangsan, Korea). Hexane (purity 95%), potassium hydroxide (purity 95%) and ethanol (purity 95%) were obtained from Samchun Chemical Co. Ltd. (Pyongtack, Korea). Cholesterol (purity 99%) was purchased from Sigma Chemical Co. (St Louis, MO, USA).
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8

ITM Flower Extract Protocol

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ITM flowers growing in Hanaro Farm (Songji-myeon, Jeollanam-do, Korea; 34°23′00.4′′ N 126°33′59.0′′ E) were collected on 12 September 2018 and identified by Jong-Cheol Yang (Baekdudaegan National Arboretum, Bonghwa-gun, Korea). A voucher specimen (No. IT-0002) was kept at the Herbarium of the College of Life and Health Science (Hoseo University, Korea). Absolute was extracted by solvent extraction method as in a previous report [16 (link)]. In brief, ITM flowers (4.05 kg) were immersed in 15 L of hexane (Samchun, Pyeongtaek, Korea) at room temperature (RT) for 1 h. Extracts were collected, and the hexane removal was carried out by a rotary evaporator (Eyla, Tokyo, Japan) at 25 °C under vacuum to obtain a dark yellow waxy residue (concrete). This residue was then dissolved in ethanol (99.5%; Samchun Chemicals, Pyungtaek, Korea), left at −20 °C for 12 h, filtered through a sintered funnel, and then evaporated at 35 °C to remove ethanol, leaving a light-yellow anhydrous wax (ITMFAb; 3.38 g, yield 0.083%, w/w). The ITMFAb obtained was stored at −80 °C until required.
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9

Colloidal Perovskite Nanocrystal Synthesis

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OLAM (98%), HBr (48%), Cs2CO3 (99.9%), PbO (99.999%), ODE (90%), OA (90%), and lead bromide (PbBr2, 98%) were purchased from Sigma‐Aldrich. Acetonitrile (ACN, 99.5%), toluene (99.8%), methyl acetate (99.5%), and hexane (99.5%) were purchased from Samchun Chemicals. All the chemicals were used without further purification.
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