FFPE mouse and/or human lung tissues were stained with antibodies against CD79α-B cell marker (M7050, Dako), B-cell activating factor-BAFF (Ab117256, Abcam) and CD21-follicular dendritic cell marker (Ab75985, Abcam) and Ki67-proliferation marker (Ab16667, Abcam) using the Bond Polymer Refine Red Detection kit on the Leica Bond Autostainer according to the manufacturer’s protocol as previously described [16 (link)]. Slides were scanned using the Aperio imaging system (Leica Biosystem; Concord, Ontario).
M7050
Manufactured by Agilent Technologies
Sourced in Germany
The M7050 is a laboratory instrument designed for the analysis and characterization of materials. It features high-resolution imaging capabilities and advanced analytical tools for researchers and scientists. The M7050 provides precise data on the physical and chemical properties of samples, enabling in-depth analysis and discovery.
Automatically generated - may contain errors
Lab products found in correlation
Ab75985, by Abcam (1 mentions)
Ab16667, by Abcam (1 mentions)
Bond polymer refine red detection kit, by Leica camera (1 mentions)
Ab117256, by Abcam (1 mentions)
Bond autostainer, by Leica camera (1 mentions)
Aperio imaging system, by Leica Biosystems (1 mentions)
Autostainer, by Leica Biosystems (1 mentions)
M7103, by Agilent Technologies (1 mentions)
Ncl l cd4 1f6, by Leica Biosystems (1 mentions)
M7158, by Agilent Technologies (1 mentions)
M0888, by Agilent Technologies (1 mentions)
M7240, by Agilent Technologies (1 mentions)
2 protocols using m7050
1
Immunohistochemistry of Lung Tissues
2
Autopsy Examination Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
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An autopsy was performed two hours after the patient’s death. No craniotomy was performed. Written informed consent was obtained from the patient’s family before autopsy. The protocol for reporting this case was approved by the ethics committee of Asahikawa Medical University (approval number #17115, August 28, 2017). The systemic organs were removed, examined, and fixed in phosphate-buffered 10 % formalin. Tissues were processed to paraffin blocks and the Section (4 μm) were subjected to hematoxylin and eosin staining, as well as Berlin blue staining. An autostainer (Leica Biosystems, Nußloch, Germany) was used to perform immunohistochemistry assessments of CD79a (antibody: M7050, DAKO, Carpinteria, CA), CD4 (antibody: NCL-L -CD4-1F6, Novocastra, Leica Microsystems, Wetzlar, Germany), CD8 (antibody: M7103, DAKO), HepPar1 (antibody: M7158, DAKO), cytokeratin 19 (CK19) (antibody: M0888, DAKO), and Ki-67 (MIB-1) (M7240, DAKO) expression.
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