Sep pak accell plus qma

Manufactured by Waters Corporation

Sourced in Ireland, United States

The Sep-Pak Accell Plus QMA is a solid-phase extraction cartridge designed for the purification and concentration of analytes from various sample matrices. It features a quaternary methylamine (QMA) sorbent material, which is useful for the retention and elution of anionic compounds. The core function of this product is to facilitate the sample preparation process prior to analysis or further processing.

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Lab products found in correlation

Sep pak c18 light cartridge, by Waters Corporation (2 mentions) Eclipse plus c18, by Agilent Technologies (2 mentions) Maldi ms daltonics microflex, by Bruker (1 mentions) Avance 3 hd 600 mhz spectrometer, by Bruker (1 mentions) Biograph mct flow 64 scanner, by Siemens (1 mentions) Micropet r4 rodent scanner, by Siemens (1 mentions) Acrodisc 25 mm syringe filter, by Pall Corporation (1 mentions) Ar 2000, by Bioscan (1 mentions) Flow count, by Bioscan (1 mentions) Pettrace cyclotron, by GE Healthcare (1 mentions) Sep pak c 18 light, by Waters Corporation (1 mentions) Glu urea lys ahx hbed cc psma 11, by Horiba (1 mentions) Lc 10ad pump, by Shimadzu (1 mentions) Nucleosil 100 5 c18 column, by Macherey-Nagel (1 mentions)

Close spelling variants of this product

Sep-Pak Accell Plus QMA carbonate Sep-Pak Accell Plus QMA Plus Light Cartridge Sep-Pak Accell Plus QMA cartridge Sep-Pak® light (46 mg) Accell™ plus QMA carbonate cartridges Sep-Pak® Light AccellTM Plus QMA Cartridge Sep-Pak Accell Plus QMA Carbonate Plus Light Cartridge Sep-Pak

6 protocols using sep pak accell plus qma

Radiolabeling and Characterization of Al^18F-PSMA-BCH

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All chemicals, reagents, and solvents were purchased commercially without further purification. PSMA was purchased from Novoprotein Scientific Inc. Sep-Pak Accell Plus QMA and Sep-Pak C18 Light cartridges were purchased from Waters, and a 96-well polystyrene Stripwell™ microplate was purchased from Costar. No-carrier-added Na¹⁸F and Al¹⁸F-PSMA-BCH were provided by the Department of Nuclear Medicine, Peking University Cancer Hospital.
The product was analyzed by reversed-phase high-performance liquid chromatography (RP-HPLC; Eclipse Plus C18, 4.5 × 250 mm, 5 μm; Agilent) performed using a linear A–B gradient (15–60% of B in 15 min) with a flow of 1 ml/min. Solvents were 0.1% aqueous trifluoroacetic acid (TFA) and 0.1% TFA in acetonitrile for A and B, respectively. The HPLC system was equipped with UV and γ detectors. UV absorbance was measured at 220 nm. Nuclear magnetic resonance was performed with a Bruker Avance III HD 600 MHz spectrometer, and mass spectrometry was performed with the MALDI-MS Daltonics Microflex (Bruker Daltonics, Bremen, Germany) system. Micro-PET was performed on a Super Argus PET scanner (Sedecal, Spain).

Liu T., Liu C., Ren Y., Guo X., Jiang J., Xie Q., Xia L., Wang F., Zhu H, & Yang Z. (2020). Development of an Albumin-Based PSMA Probe With Prolonged Half-Life. Frontiers in Molecular Biosciences, 7, 585024.

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Purification and Characterization of Hydroxylated BDEs

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The hydroxylated BDEs, 6-OH-BDE47, 2-OH-BDE68, 4-OH-BDE68, 2-OH,6’-MeO-BDE68 (Figure 2) were isolated from marine Dysideidae sponges (Agarwal et al., 2015 (link)) or synthesized as described previously (Marsh et al., 2003 ). Each compound was characterized for purity by NMR and mass spectrometry, as detailed previously (Agarwal et al., 2015 (link)).
The uridine diphospho-¹⁴C-glucuronic acid (UDPGA), specific activity 250 mCi/mmole and 3′-phosphoadenosine-5′-phospho-³⁵S-sulfate (PAPS), specific activity 2.99 Ci/mmole were purchased from Perkin-Elmer, Waltham, MA and diluted with unlabeled UDPGA (Sigma-Aldrich) or PAPS (Sigma) for use in assays. The unlabeled PAPS from Sigma contained PAP and therefore was purified using an anion-exchange cartridge (Sep-pak Accell plus QMA, Waters, Milford, MA) and eluting with increasing concentrations of sodium chloride. The eluted fractions were analyzed by HPLC using an anion-exchange column, Zorbax SAX, 4.6 × 250 mm, 5 μ (Agilent) with a mobile phase of 0.25 M ammonium phosphate pH 3.0, flow rate 1 mL/min and UV detection at 260 nm, selective for adenosine. Under these conditions PAP eluted at 5.0 min and PAPS at 7.5 min. Fractions shown by HPLC to contain >90% PAPS, i.e. UV₂₆₀ peak at 7.5 min and no PAP were used in assays. All other chemicals were purchased from Sigma–Aldrich or Fisher Scientific (Orlando, FL).

Cisneros K.V., Agarwal V, & James M.O. (2019). Sulfonation and Glucuronidation of Hydroxylated Bromodiphenyl Ethers in Human Liver. Chemosphere, 226, 132-139.

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Radiolabeled Theranostic Peptide for PET/CT and PET/MRI Imaging

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All solvents and chemicals purchased from commercial sources were of analytical grade or better and were used without further purification. DX600, NODAGA‐DX600, and DOTA‐DX600 were custom synthesized by ChinaPeptides Co., Ltd (Shanghai, China) or CSBio (San Diego, California). Sep‐Pak Accell Plus QMA and Sep‐Pak C18‐Light cartridges were purchased from Waters (Ireland). Acrodisc 25 mm syringe filter (0.22 µm) was purchased from Pall Corporation (USA). The product was analyzed by radio‐ high performance liquid chromatography (HPLC) (1200, Agilent, USA) equipped with γ detector (Flow‐count, Bioscan, Washington. D.C., USA), using a C18 column (Eclipse Plus C18, 4.5 × 250 mm, 5µm, Agilent, USA). The product purity was also determined using Radio‐TLC (AR 2000, Bioscan, USA) after radiolabeling. The PET/CT imaging studies of small animals were performed on the Mira PET/CT of PINGSENG Healthcare Inc. (Shanghai, China), or microPET R4 rodent scanner (Siemens) and analyzed by ASIProVM. The Clinical PET/CT scans were obtained on a Biograph mCT Flow 64 scanner (Siemens, Erlangen, Germany) with unenhanced low‐dose CT. ⁶⁸Ga‐HZ20 PET/MRI was performed on a hybrid 3.0T PET/MR scanner (uPMR790, UIH, Shanghai, China) in female volunteers.

Zhu H., Zhang H., Zhou N., Ding J., Jiang J., Liu T., Liu Z., Wang F., Zhang Q., Zhang Z., Yan S., Li L., Benabdallah N., Jin H., Liu Z., Cai L., Thorek D.L., Yang X, & Yang Z. (2021). Molecular PET/CT Profiling of ACE2 Expression In Vivo: Implications for Infection and Outcome from SARS‐CoV‐2. Advanced Science, 8(16), 2100965.

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Radiolabeling of PSMA-11 with F-18

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Glu-urea-Lys (Ahx)-HBED-CC (PSMA-11) was purchased from ABX (Radeberg, Germany). AlCl₃.6H2O, sodium acetate trihydrate, and acetic acid were all metal-free. HPLC eluents, water, acetonitrile, and trifluoroacetic acid (TFA) were of high-grade purity. Sep-Pak Accell Plus QMA and Sep-Pak C18-Light cartridges were from Waters. No-carrier-added ¹⁸F-fluoride was produced with the PETtrace cyclotron (GE Medical Systems, Uppsala) via an ¹⁸O (p,n) ¹⁸F reaction.

Boschi S., Lee J.T., Beykan S., Slavik R., Wei L., Spick C., Eberlein U., Buck A.K., Lodi F., Cicoria G., Czernin J., Lassmann M., Fanti S, & Herrmann K. (2016). Synthesis and preclinical evaluation of an Al18F radiofluorinated GLU-UREA-LYS(AHX)-HBED-CC PSMA ligand. European journal of nuclear medicine and molecular imaging, 43(12), 2122-2130.

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Preparation of PC 16:0/22:6;OOH Isomers

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PC 16:0/22:6 was determined as a target since it was abundant in mackerel based on the analysis of the extracted phospholipid fraction. A mixture of its hydroperoxide isomers (i.e., PC 16:0/22:6;OOH isomers) was prepared as a model sample of PC-DHA;OOH.
DHA (399 mg) was esterified to LPC 16:0/0:0 (200 mg) in chloroform (26.9 mL) containing DCC (812 mg) and DMAP (234 mg) under N₂ gas for 28 h at 40 °C^{22 (link)}. After the reaction, the phospholipid fraction was collected with Sep-Pak Vac Silica (35 cc, 10 g, Waters, MA, USA)^{17 (link)}. The PC 16:0/22:6 was chromatographically purified as in our previous report^{23 (link)}. PC 16:0/22:6 was further purified by removing the salt contained in the collected fraction. The purified PC 16:0/22:6 was dissolved into methanol (20 mL).
Rose bengal (0.15 mg) was added to the PC solution, and photo-oxidation was carried out by exposing the solution to LED irradiation (6000 lx, on ice) for 3 h. Rose bengal was removed with Sep-Pak Accell Plus QMA (360 mg, Waters). The eluent was dried under N₂ gas. PC 16:0/22:6;OOH was purified in the same manner as the PC 16:0/22:6 purification. The total weight of obtained PC 16:0/22:6;OOH isomers was 1.6 mg after the collected eluent was dried. The sample was dissolved in chloroform (200 μL) and stored at − 80 °C until the analysis. The headspace of the sample vial was filled with N₂ gas prior to storage.

Kusumoto I., Kato S, & Nakagawa K. (2023). Analysis of docosahexaenoic acid hydroperoxide isomers in mackerel using liquid chromatography–mass spectrometry. Scientific Reports, 13, 1325.

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Quantification of Fumonisin B1 by HPLC

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FB
₁was determined by high-performance liquid chromatography (HPLC) according to Shephard et al.(1990)
with some modification (
Ueno et al., 1993
).
One milliliter of the cell-free extract previously mixed with 1 mL methanol-water (3:1, v/v) was applied onto a preconditioned Sep Pak accell plus QMA (quaternary methylammonium) cartridge (Waters Co., USA). After washing the cartridge with methanol-water (3:1, 6 mL) followed by methanol (3 mL), FB
₁was eluted with 10 mL methanol containing 0.5% acetic acid. The eluate was evaporated to dryness under a stream of nitrogen at 45 °C, and the residue was dissolved in methanol-water (3:1, 800 μL). After derivatization with 200 μL o-phthaldialdehyde (OPA) reagent, HPLC injections were made within 1 min. FB
₁was analyzed by a reversed-phase, isocratic HPLC system (Shimadzu LC-10 AD pump and RF-10A XL fluorescence detector (Shimadzu, Japan), using a C18 Nucleosil 100-5 column (4.6 × 250 mm, Macherey-Nagel GmbH & Co., Germany). Excitation and emission wavelengths were 335 nm and 450 nm, respectively. The eluent was CH
₃OH: 0.1 M NaH
₂PO
₄(80:20, v/v) adjusted to pH 3.3 with ortho-phosphoric acid at 1 mL/min flow rate. The detection limit for FB
₁was 27.5 ng/mL.

Miguel T.D., Bordini J.G., Saito G.H., Andrade C.G., Ono M.A., Hirooka E.Y., Vizoni É, & Ono E.Y. (2015). Effect of fungicide on Fusarium verticillioides mycelial morphology and fumonisin B 1 production. Brazilian Journal of Microbiology, 46(1), 293-299.

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