GC-1spg cells (ATCC, cat # CRL-2053) with inducible DAZL expression were maintained in DMEM high glucose medium (ThermoFisher) supplemented with 10% (v/v) Tet-system approved FBS (Clontech), 100 U/mL penicillin, 100 mg/mL streptomycin, 5 mg/mL blasticidin, and 300 mg/mL Zeocin (all from ThermoFisher) at 37°C, 5% (v/v) CO2 (ref.17 (link)). Cells tested negative for mycoplasma contamination with the MycoAlert Mycoplasma Detection System (Lonza, cat # LT07-118) and the MycoAlert Assay Control Set (Lonza, cat # LT07-518) as a positive control. Doxycycline induction of Dazl was performed and lysates for generation of cDNA libraries and quantification of Dazl levels were prepared as described 17 (link). Equal amounts of protein were run on a SDS-PAGE (10% NEXT Gel, Amresco) and transfered to a PVDF membrane. Western blotting was performed with anti-Dazl (Rabbit; 1:5000, US Biological) and anti-Hsp90 (Rabbit; 1:10,000; US Biological) antibodies. Chemiluminescence was quantified with the ImagequantTL software.
Anti dazl rabbit
Manufactured by US Biological
Anti-Dazl (Rabbit) is a polyclonal antibody that detects the DAZL protein, which is a member of the DAZ (Deleted in Azoospermia) family of RNA-binding proteins essential for germ cell development. This antibody is commonly used in research applications to study the expression and localization of the DAZL protein in various cell types and tissues.
Automatically generated - may contain errors
Lab products found in correlation
Zeocin, by Thermo Fisher Scientific (3 mentions)
Tet system approved fbs, by Takara Bio (3 mentions)
Dmem high glucose medium, by Thermo Fisher Scientific (3 mentions)
Next gel, by Avantor (3 mentions)
Blasticidin, by Thermo Fisher Scientific (2 mentions)
Mycoalert assay control set, by Lonza (2 mentions)
Mycoalert mycoplasma detection system, by Lonza (2 mentions)
3 protocols using anti dazl rabbit
1
Inducible DAZL Expression in GC-1spg Cells
2
Inducible DAZL Expression in GC-1spg Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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GC-1spg cells (ATCC, cat # CRL-2053) with inducible DAZL expression were maintained in DMEM high glucose medium (ThermoFisher) supplemented with 10% (v/v) Tet-system approved FBS (Clontech), 100 U/mL penicillin, 100 mg/mL streptomycin, 5 mg/mL blasticidin, and 300 mg/mL Zeocin (all from ThermoFisher) at 37°C, 5% (v/v) CO2 (ref.17 (link)). Cells tested negative for mycoplasma contamination with the MycoAlert Mycoplasma Detection System (Lonza, cat # LT07-118) and the MycoAlert Assay Control Set (Lonza, cat # LT07-518) as a positive control. Doxycycline induction of Dazl was performed and lysates for generation of cDNA libraries and quantification of Dazl levels were prepared as described 17 (link). Equal amounts of protein were run on a SDS-PAGE (10% NEXT Gel, Amresco) and transfered to a PVDF membrane. Western blotting was performed with anti-Dazl (Rabbit; 1:5000, US Biological) and anti-Hsp90 (Rabbit; 1:10,000; US Biological) antibodies. Chemiluminescence was quantified with the ImagequantTL software.
3
Dazl Expression and Quantification in GC-1spg Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
GC-1spg cells with inducible DAZL expression were maintained in DMEM high glucose medium (ThermoFisher) supplemented with 10% (v/v) Tet-system approved FBS (Clontech), 100 U/mL penicillin, 100 mg/mL streptomycin, 5 mg/mL blasticidin, and 300 mg/mL Zeocin (all from ThermoFisher) at 37°C, 5% (v/v) CO 2 (ref. 17 ). Doxycycline induction of Dazl was performed and lysates for generation of cDNA libraries and quantification of Dazl levels were prepared as described 17 . Equal amounts of protein were run on a SDS-PAGE (10% NEXT Gel, Amresco)
and transfered to a PVDF membrane. Western blotting was performed with anti-Dazl (Rabbit;
1:5000, US Biological) and anti-Hsp90 (Rabbit;1:10,000; US Biological) antibodies.
Chemiluminescence was quantified with the ImagequantTL software.
and transfered to a PVDF membrane. Western blotting was performed with anti-Dazl (Rabbit;
1:5000, US Biological) and anti-Hsp90 (Rabbit;1:10,000; US Biological) antibodies.
Chemiluminescence was quantified with the ImagequantTL software.
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