Periplasmic hBMP-2 and samples from the purification steps were analyzed by 15% polyacrylamide gel electrophoresis (SDS-PAGE) under non-reducing conditions, as previously described, Coomassie Brilliant Blue G-250 being used for the staining [31 (link)]. For Western blotting, the semi-dry transfer technique on nitrocellulose membrane was used, with anti-hBMP-2 affinity-purified rabbit IgG, 1:2000 (Biovision, Milpitas, CA, USA) and goat anti-rabbit IgG conjugated to horseradish peroxidase (1:5000). Proteins were visualized with Luminata Forte (Merck, Burlington, MA, USA) on CL-Xposure TM Film (Thermo Scientific, Rockford, IL, USA).
Anti hbmp 2 affinity purified rabbit igg
Manufactured by Abcam
Sourced in United States
Anti-hBMP-2 affinity-purified rabbit IgG is a laboratory reagent used for research purposes. It is an affinity-purified immunoglobulin G (IgG) antibody raised in rabbits against human bone morphogenetic protein 2 (hBMP-2).
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using anti hbmp 2 affinity purified rabbit igg
1
Purification and Analysis of hBMP-2
2
Comparative Analysis of Recombinant BMP-2
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E. coli-derived met-hBMP-2 (GenScript) and CHO-derived hBMP-2 (Infuse®) were analyzed under reducing and non-reducing conditions (Soares et al. 2000 (link)). Coomassie Brilliant Blue G-250 was used for the staining. For Western blotting, the semi-dry transfer technique was utilized on a nitrocellulose membrane, with anti-hBMP-2 affinity-purified rabbit IgG (1:2000) (Biovision, Milpitas, CA, USA) and goat anti-rabbit IgG conjugated to horseradish peroxidase (1:5000). Protein visualization was performed with Luminata Forte (Merck, Burlington, MA, USA) on X-ray film (CL-Xposure™ Film, Thermo Scientific, Rockford, IL, USA).
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