Cells were seeded onto Ibidi µ-Slide 8 Well overnight and treated with or without verteporfin or CA3 for 48 h, fixed with 4% paraformaldehyde and permeabilized with 0.5% Triton. Samples were incubated with Anti-YAP antibody (Cell signaling Technology, Leiden, The Netherlands). F-actin and nucleus were stained using respectively Alexa-fluor 488 phalloidin and DAPI. Images were acquired using a confocal microscope (NIKON A1 N-SIM) and processed using ImageJ.
A1 n sim
Manufactured by Nikon
Sourced in Germany
The Nikon A1 N-SIM is a super-resolution microscope system designed for advanced imaging applications. It utilizes structured illumination microscopy (SIM) technology to achieve up to 2x higher resolution compared to conventional optical microscopes, allowing for detailed visualization of subcellular structures. The core function of the A1 N-SIM is to provide high-resolution, non-invasive imaging capabilities for life science research and other applications requiring enhanced spatial resolution.
Automatically generated - may contain errors
Lab products found in correlation
Anti yap antibody, by Cell Signaling Technology (2 mentions)
Anti vinculin fitc antibody, by Merck Group (2 mentions)
Duolink in situ reagents, by Merck Group (1 mentions)
Nucleospin rna plus, by Macherey-Nagel (1 mentions)
Quantstudio 7 flex real time pcr system, by Thermo Fisher Scientific (1 mentions)
Sybr select master mix, by Thermo Fisher Scientific (1 mentions)
4 protocols using a1 n sim
1
Verteporfin and YAP Localization Assay
2
Protein Interaction Detection via Duolink PLA and Immunofluorescence
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Duolink PLA ®: 5 × 103 OS cells were seeded in Ibidi µ-Slide VI 0.4. 24 later, media was changed to DMEM with 1% FBS. Cells were then fixed with 4% PFA for 15 min at room temperature and incubated overnight at 4 °C with primary antibody against YAP (Cell signaling or Santa Cruz Biotechnology), TEF-1 (Santa Cruz). In situ PLA was performed using DuoLink in Situ Reagents (Sigma-Aldrich) according to the manufacturer’s instructions.
Immunofluorescence assays: cells were seeded onto Ibidi µ-Slide 8 Well overnight, fixed with 4% paraformaldehyde for 15 min and permeabilized with 0.5% Triton. Samples were incubated with Anti-Vinculin−FITC antibody (Sigma-Aldrich). F-actin and nucleus were stained using respectively Alexa-fluor 488 phalloidin and DAPI. Images were acquired using a confocal microscope (NIKON A1 N-SIM) and processed using ImageJ.
Immunofluorescence assays: cells were seeded onto Ibidi µ-Slide 8 Well overnight, fixed with 4% paraformaldehyde for 15 min and permeabilized with 0.5% Triton. Samples were incubated with Anti-Vinculin−FITC antibody (Sigma-Aldrich). F-actin and nucleus were stained using respectively Alexa-fluor 488 phalloidin and DAPI. Images were acquired using a confocal microscope (NIKON A1 N-SIM) and processed using ImageJ.
3
Visualization of Focal Adhesions
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were seeded onto Ibidi µ-Slide 8 Well overnight, fixed with 4% paraformaldehyde for 15 min, and permeabilized with 0.5% Triton. The samples were incubated with anti-vinculin−FITC antibody (Sigma-Aldrich, St. Quentin-Fallavier, France) for 2 h at room temperature (1:200) and then washed in phosphate-buffered saline. F-actin and the nucleus were stained using Alexa-fluor 488 phalloidin (1:1,000) and DAPI (1:1,000), respectively. The images were acquired using a confocal microscope (NIKON A1 N-SIM) and processed using ImageJ.
4
Immunofluorescence and qRT-PCR of YAP Signaling
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Cells were seeded onto Ibidi µ-Slide 8 Well overnight and treated with or without vertepor n or CA3 for 48h, xed with 4% paraformaldehyde and permeabilized with 0.5% Triton. Samples were incubated with Anti-YAP antibody (Cell signaling Technology, Leiden, The Netherlands). F-actin and nucleus were stained using respectively Alexa-uor 488 phalloidin and DAPI. Images were acquired using a confocal microscope (NIKON A1 N-SIM) and processed using ImageJ. RNA extraction and Real-time polymerase chain reaction RNA was extracted from cells and tumors using NucleoSpin®RNAplus (Macherey Nagel, Duren, Germany) and reverse transcribed using the Maxima H minus rst stand cDNA synthesis kit (Thermo Fisher, Courtaboeuf, France). Real-time monitoring of PCR ampli cation of complementary DNA was performed using DNA primers (primer sequences are available in Table 1) using QuantStudio 7 Flex Real-Time PCR System (Thermo Fisher) with SYBR® Select Master Mix (Life Technologies, Carlsbad, CA). Target gene expression was normalized to glyceraldehyde 3-phosphatedehydrogenase (GAPDH) and β-actin (ACTB) levels in respective samples as an internal standard.
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!