Trans, trans-FOH, dimethyl sulfoxide (DMSO), 6-(4-chlorophenyl)imidazo[2,1-b][1,3]thiazole-5-carbaldehyde O-(3,4-dichlorobenzyl)oxime (CITCO), 3-[2-[2-chloro-4-[[3-(2,6-dichlorophenyl)-5-(1-methylethyl)-4 isoxazolyl]methoxy]phenyl]ethenyl]benzoic acid (GW4064), OA, hematoxylin, Oil Red O (ORO), 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), triamcinolone acetonide, Triton X-100, and fatty acid-free bovine serum albumin (FF-BSA) were purchased from Sigma-Aldrich (St. Louis, MO). 2-[[4-[2-[[(Cyclohexylamino)carbonyl](4-cyclohexylbutyl)amino]ethyl]phenyl]thio]-2-methylpropanoic acid (GW7647) and N-((2S)-2-(((1Z)-1-methyl-3-oxo-3-(4-(trifluoromethyl)phenyl)prop-1-enyl)amino)-3-(4-(2-(5-methyl-2-phenyl-1,3-oxazol-4 (GW6471) were purchased from Bio-Techne (Minneapolis, MN). Williams’ Medium E, Penicillin-Streptomycin solution, and fetal bovine serum (FBS) were purchased from Invitrogen (Carlsbad, CA), and recombinant human insulin (Novolin R) from Novo Nordisk Pharmaceuticals, Inc. (Princeton, NJ). Tritiated OA ([9, 10-3H(N)]OA, 15–60Ci (0.555–2.22TBq)/mmol) was purchased from PerkinElmer (Waltham, MA). Other materials were obtained from the sources indicated below.
Fatty acid free bovine serum albumin ff bsa
Manufactured by Merck Group
Sourced in United States
Fatty acid-free bovine serum albumin (FF-BSA) is a laboratory product used as a protein supplement in cell culture media. It is derived from bovine serum and has been processed to remove fatty acids. FF-BSA provides a source of amino acids, peptides, and other nutrients to support cell growth and maintenance in in vitro experiments.
Automatically generated - may contain errors
Lab products found in correlation
Triton x 100, by Merck Group (1 mentions)
William s e medium, by Thermo Fisher Scientific (1 mentions)
Fetal bovine serum (fbs), by Thermo Fisher Scientific (1 mentions)
Hematoxylin, by Merck Group (1 mentions)
Triamcinolone acetonide, by Merck Group (1 mentions)
Penicillin streptomycin solution, by Thermo Fisher Scientific (1 mentions)
6 4 chlorophenyl imidazo 2 1 b 1 3 thiazole 5 carbaldehyde o 3 4 dichlorobenzyl oxime, by Merck Group (1 mentions)
Total erk1 2, by Cell Signaling Technology (1 mentions)
C1 bodipy 500 510 c12 4 4 difluoro 5 methyl 4 bora 3a 4a diaza s indacene 3 dodecanoic acid, by Thermo Fisher Scientific (1 mentions)
Imatinib mesylate, by Selleck Chemicals (1 mentions)
Vegf b186, by R&D Systems (1 mentions)
2 protocols using fatty acid free bovine serum albumin ff bsa
1
Liver Cell Culture Protocol
2
VEGF-B186 and Lipid Tracking in Endothelial Cells
Check if the same lab product or an alternative is used in the 5 most similar protocols
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Vascular endothelial growth factor- B 186 (VEGF-B186) was obtained from R&D systems (Abingdon, Oxfordshire, UK). It was dissolved in phosphate-buffered saline (PBS) to a stock concentration of 30ug/ml [9 (link)]. C1-BODIPY®500/510 C12(4,4-Difluoro-5-Methyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) was from Thermo Fisher Scientific (Waltham, Massachusetts, United States) and dissolved in dimethyl sulfoxide (DMSO) to a stock concentration of 2mM [9 (link)]. Imatinib mesylate was from SelleckChem (Houston, TX) and dissolved in dimethyl sulfoxide (DMSO) to a stock concentration of 10mM [20 (link)]. Fatty acid-free bovine serum albumin (FF-BSA) was obtained from Sigma-Aldrich (St. Louis, Missouri, United States). The different antibodies used for western blotting i.e. anti-pTyr207CrkL (#3181), anti-CrkL (#3182), phosphor ERK1/2 and total ERK1/2 were from Cell Signalling Technology (Danvers, United States). Human umbilical cords from healthy donors were obtained from Amstelveen Hospital, Amstelveen, Netherlands.
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