TRIzol reagent (Thermo Fisher Scientific, cat# 15596018), TRIzol LS Reagent (Ambion, Cat# 10296010), chloroform (Sigma-Aldrich, Cat No. C2432), glycogen (Thermo Fisher Scientific, cat# R0551), 3 M sodium acetate (pH5.2, Quality Biological, cat# 351035721), ethyl alcohol (Thermo Scientific Richard-Allan Scientific, for in vitro diagnostic use), isopropyl alcohol (Thermo Scientific Richard-Allan Scientific, for in vitro diagnostic use), nuclease-free water (Cell Signaling Technology, cat# 12931S), mixture vortex (VWR, Analog Vortex Mixer), and centrifuge (Thermo Scientific, Legend Micro 21R). MagMAX Blood RNA Isolation Kit (ThermoFisher Scientific, Cat# AM1837), NucleoSpin for miRNA and RNA purification kit (Macherey-Nagel, Cat# 740971.50), and Plasma/Serum RNA Purification kit (Norgen Biotek Corp, Cat# 56100).
Analog vortex mixer
Manufactured by Thermo Fisher Scientific
Sourced in United States
The Analog Vortex Mixer is a compact, benchtop lab equipment designed to mix liquids and other samples in a vortex motion. It features a variable speed control knob to adjust the mixing intensity. The mixer's compact size and simple operation make it a versatile tool for various laboratory applications.
Automatically generated - may contain errors
Lab products found in correlation
Trizol reagent, by Thermo Fisher Scientific (2 mentions)
Precisiontm stool collectors, by Thermo Fisher Scientific (2 mentions)
903 protein saver cards, by GE Healthcare (2 mentions)
Synergy h4 microplate reader, by Agilent Technologies (2 mentions)
Glycogen, by Thermo Fisher Scientific (1 mentions)
Sodium acetate, by Quality Biological (1 mentions)
Legend micro 21r, by Thermo Fisher Scientific (1 mentions)
Plasma serum rna purification kit, by Norgen Biotek (1 mentions)
Trizol ls reagent, by Thermo Fisher Scientific (1 mentions)
Chloroform, by Merck Group (1 mentions)
Nanodrop lite spectrophotometer, by Thermo Fisher Scientific (1 mentions)
Titramax plateshaker, by Heidolph (1 mentions)
Meso quickplex sq 120, by MSD (1 mentions)
Whatman 3 filter paper, by GE Healthcare (1 mentions)
Ab9203, by Abcam (1 mentions)
Ab30384, by Abcam (1 mentions)
Tmb one, by Promega (1 mentions)
Dynabeads protein g, by Thermo Fisher Scientific (1 mentions)
15 protocols using analog vortex mixer
1
RNA Extraction from Biological Samples
2
Peptide Concentration Validation and Quantification
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NanoDrop Lite Spectrophotometer (Thermo Fisher Scientific, Waltham, MA) to validate peptide concentrations
Titramax plate shaker (Heidolph, Schwabach, Germany) for vigorous plate shaking, per manufacturer's instructions
MESO QuickPlex SQ 120 (MSD) for data acquisition
Fisherbrand™ Analog Vortex Mixer (Thermo Fisher Scientific)
3
Stool Collection from Allogeneic HCT Patients
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Stools from allogeneic hematopoietic cell transplantation (HCT) patients were collected at multiple time points during the patient’s first 100 days post-transplant, both during hospitalisation by nurses and at home following discharge, in a hat that sits on the toilet seat. Caregivers/patients were instructed to collect bowel movements with a maximum of two per day and to collect a sample of ‘native’ stool for Bristol scoring, as well as to scoop stool into PrecisionTM Stool Collectors preloaded with 50 ml EDTA without glass beads for subsequent DNA analysis. The specimens were then delivered to the laboratory within 48 hours of bowel movement, at which time the native sample was assessed for Bristol score and the EDTA-stabilised sample was further processed as follows: Stool weight to buffer volume ratios were not adjusted. Stools were homogenised in the PrecisionTM Stool Collectors by vortexing on a Fisherbrand™ Analog Vortex Mixer (Catalog No. 02-215-365) on setting 9 until the slurry appeared homogenous to visual inspection. The stool slurries were aliquoted and frozen at −80 °C.
4
Seed-Mediated Synthesis of Spherical AuNPs
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Spherical AuNPs were synthesized in two steps by a seed-mediated approach using cetyl tri-methyl ammonium chloride (CTAC) as a surfactant. In the first step, seed solution was prepared by vigorous mixing (500 rpm for 1 h at 30 °C; using analog vortex mixer (Fisher Scientific, USA) of 10 mL of HAuCl4 (2.5 × 10–4 M) aqueous CTAC (0.1 M) solution with 0.45 mL of cold NaBH4 (0.02 M). Further, the seed solution was aged for 60 minutes (at 30 °C) to decompose the excess NaBH4. In the second step, the growth solution was prepared by adding 514 μL of HAuCl4 (4.86 mM), 10 μL of NaBr (0.01 M), and 90 μL of ascorbic acid (0.04 M) to 5 mL of aqueous CTAC (0.1 M) solution. Finally, 25 μL of the seed solution was added to a colorless growth solution, under vigorous agitation, and left undisturbed overnight at room temperature. To control the size of the AuNPs, the molar ratio of the ascorbic acid to the seed solution was varied.
5
Efficient Vortex Mixing Protocol
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Collection tubes were vortexed vigorously on a Fisherbrand™ Analog Vortex Mixer (Catalog No. 02–215–365) at setting 9 for 30 seconds.
6
HRP2-based Malaria ELISA Assay
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Human Whole Blood (K3 EDTA) was purchased from Bioreclamation IVT (Catalog No. HMWBEDTA3). Plasmodium falciparum D6 parasite was cultured in house. Recombinant HRP2 protein (rc-HRP2) for traditional ELISA standard curve was purchased from Immunology Consultants Laboratory Inc. (Catalog No. AGPF-55). ELISA capture and detection antibodies were acquired from Abcam Inc. (Catalog Nos. ab9206 and ab30384). Immulon 2HB ELISA plates (Catalog No. 14-245-61) and TMB One ELISA substrate (Catalog No. PR-G7431) were purchased from Fisher Scientific. 903 Protein Saver cards and Whatman 3 filter paper, were purchased from GE Healthcare Life Sciences (Catalog Nos. 10534612 and 1003-055). Biopunches, 6 mm, were acquired from Ted Pella Inc. (Catalog No. 15111-60). All other reagents were purchased from either Fisher Scientific or Sigma Aldrich. DBS extraction was performed with a Fisher Scientific Analog Vortex Mixer (Catalog No. 02-215-365). Absorbance measurements were collected on a Biotek Synergy H4 microplate reader (Vanderbilt University) or Biotek ELx808 microplate reader (Macha Research Trust).
7
High-Pressure Processing of Whole Milk Foam
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EXAMPLE 3
Whole milk (3.98% fat; 5.02% total solids) was processed using the high pressure jet (previously described) at pressures 0, 125, 250, 375, and 500 MPa. After processing, milk was vortexed (Fisher Scientific, Analog Vortex Mixer) for one minute. The percent foam expansion was calculated immediately after processing and one hour after foaming, using the following formula:
Where Vv is the volume of milk after vortexing and Vo is the initial volume of milk (10 mL)
8
Electrospun Mat Coating Procedure
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A CS solution was prepared by first dissolving CS in 0.5 M AA at 2.5% w/v ratio and then diluting to a 0.5% w/v CS solution in 0.5 M AA. PAA and pDADMAC solutions were prepared by diluting the as-received stock solution to 0.5 w/v% in DI water. Before use, each diluted solution was first vortexed using a Fisher Scientific Analog Vortex Mixer (02215365). To coat, first an electrospun mat was punched into a circle with a 2.54 cm diameter using a Spearhead® 130 Power Punch MAXiset (Fluid Sealing Services, Wausau, WI, USA). Six mats at a time were submerged in a square petri dish containing 20 mL of one of the coating solutions described above. The petri dish was then placed onto a 120 V Lab Line 3-D rotator (model #4630, Thermo Scientific, Waltham, MA, USA) for 30 min. After rotation, the mats were removed from solution, rinsed with DI water, and dried for 24 h.
9
Stool Homogenization and Buffer Ratio
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Stool weight to buffer volume ratios were adjusted to 1:4 by adding additional TEN2 buffer if stool weight was > 10 g. Buffer was not added if the stool weight was < 10 g. Stools were homogenised in PrecisionTM Stool Collectors by vortexing on a Fisherbrand™ Analog Vortex Mixer (Catalog No. 02-215-365) on setting 9 until the sample appeared homogenous to visual inspection.
10
HRP2-based Malaria Diagnostic Assay
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Human whole blood (CPD) was purchased from Bioreclamation IVT (catalog no. HMWBCPD). Recombinant HRP2 protein (rcHRP2) was generously provided by PATH (Seattle, WA). Plasmodium falciparum D6 strain was cultured in-house. Dynabeads® Protein A, Dynabeads® Protein G, and Pierce Protein L Magnetic Particles were purchased from Fisher Scientific (10-002-D, 10-004-D, PI88850). Anti-HRP2 antibodies were purchased from Abcam (ab9203, ab9206, ab30384). TMB One was purchased from Promega (G7431). 903 Protein Saver Cards were purchased from GE Healthcare Life Sciences (10534612). A Fisher Scientific Analog Vortex Mixer (02-215-365) was used for all vortexed incubations. A VWR Digital Dry Heatblock (12621-086) with an external thermocouple (11301-112) was used for sample heating. Absorbance was measured on a Biotek Synergy H4 microplate reader (Vanderbilt University) or Biotek ELx808 microplate reader (Macha Research Trust). All other reagents and materials were purchased from either Fisher Scientific or Sigma Aldrich.
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