Anti rabbit trueblot igg hrp conjugate antibody

Manufactured by Thermo Fisher Scientific

Sourced in Germany, France

The Anti-rabbit Trueblot IgG HRP conjugate antibody is a secondary antibody that binds to rabbit primary antibodies. It is conjugated with horseradish peroxidase (HRP) enzyme, which can be used for detection in various immunoassay applications.

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Lab products found in correlation

Mouse anti vinculin antibody, by Merck Group (2 mentions) Anti rabbit igg hrp conjugate antibody, by Santa Cruz Biotechnology (2 mentions) Rabbit anti eif4e antibody, by Cell Signaling Technology (2 mentions) Re blot plus mild solution, by Merck Group (2 mentions) Mouse anti polyhistidine antibody, by Merck Group (1 mentions)

Close spelling variants of this product

IgG Rabbit (4 citations)

2 protocols using anti rabbit trueblot igg hrp conjugate antibody

Western Blot Analysis of Stress Proteins

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

Western blot analyses were performed using a previously published method [15 (link)]. The following antibodies were used: rabbit Hsp27 antibody (Assay Designs, Villeurbanne, France, 1/5000), rabbit anti-eIF4E antibody (Cell Signaling, Ozyme, 1/1000, Saint-Cyr-l’école, France), anti-rabbit IgG HRP conjugate antibody (Santa Cruz Biotechnology, Heidelberg, Germany, 1/5000), and anti-rabbit Trueblot IgG HRP conjugate antibody (eBiosciences, 1/1000, Villebon-sur-Yvette, France). Loading levels were normalized using mouse anti-vinculin antibody (Sigma-Aldrich, 1/2000, Saint-Quentin-Fallavier, France). Re-blot Plus Mild Solution (Millipore, Molsheim, France) was used for membrane stripping during 9 min at RT.

Ziouziou H., Paris C., Benizri S., Le T.K., Andrieu C., Nguyen D.T., Appavoo A., Taïeb D., Brunel F., Oueslati R., Siri O., Camplo M., Barthélémy P, & Rocchi P. (2021). Nucleoside-Lipid-Based Nanoparticles for Phenazine Delivery: A New Therapeutic Strategy to Disrupt Hsp27-eIF4E Interaction in Castration Resistant Prostate Cancer. Pharmaceutics, 13(5), 623.

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Optimized Western Blot Protocol

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Western blot (WB) was performed with 1/3000 mouse anti-polyHistidine antibody (Sigma-Aldrich, USA), 1/1000 rabbit anti-eIF4E antibody (Cell Signaling, Ozyme), 1/2500 anti-mouse IgG HRP conjugate antibody (Promega, France), 1/1000 anti-rabbit Trueblot IgG HRP conjugate antibody and 1/1000 anti-mouse Trueblot IgG HRP conjugate antibody (eBiosciences), 1/5000 anti-rabbit IgG HRP conjugate antibody (Santa Cruz Biotechnology, Germany), 1/5000 rabbit Hsp27 antibody (Assay Designs, FranceLoading levels were normalized using 1/2000 mouse anti-vinculin antibody (Sigma-Aldrich). Re-blot Plus Mild Solution (Millipore, France) was used for membrane stripping during 9 min at RT.

Ziouziou H., Andrieu C., Laurini E., Karaki S., Fermeglia M., Oueslati R., Taieb D., Camplo M., Siri O., Pricl S., Katsogiannou M, & Rocchi P. (2017). Targeting Hsp27/eIF4E interaction with phenazine compound: a promising alternative for castration-resistant prostate cancer treatment. Oncotarget, 8(44), 77317-77329.

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