Glibenclamide

MT4 cells were propagated in RPMI1640 supplemented with 10% (v/v) fetal bovine serum (FBS). TZM-bl and HEK-293T cells were propagated in Dulbecco’s modified Eagle’s medium (DMEM) containing 10% (v/v) FBS. HIV-1 molecular clone NL4-3 (a kind gift from Dr. Eric O Freed, NCI, NIH, USA) was used to produce infectious virus by transfecting HEK293T cells. For transfection, the cells were seeded in 6 well tissue culture plate at 0.3 × 10⁶ cells/well. At about 70% confluency, the cells were transfected with 1 μg DNA using Lipofectamine 2000 (Invitrogen, USA). The supernatant was harvested and filtered with 0.45 μm membrane filter. Viral p24 concentration was measured by using a p24 antigen capture ELISA kit (ABL, USA). Potassium chloride (cat: P9541) and cobalt chloride (cat: 232696) was purchased from Sigma-Aldrich,USA. Tetraethylammonium chloride (cat: T0095) and Glibenclamide (cat: G0382) was purchased from TCI chemicals (Japan). Barium chloride dihydrate (cat: 101719) and Ifenprodil (cat: 17201) was purchased from Cayman chemical company (USA) and Merck Life sciences (USA) respectively. 4-Aminopyridine (cat: J61470) was purchased from Alfa-Aesar (Britain). Potassium sulfate (cat: GRM1403) was purchased from HiMedia (India) Ifenprodil and Glibenclamide were dissolved in DMSO while others were dissolved in MQ water.

Ethanol (Sigma Aldrich) and distilled water were used for the extraction of the plant materials. ABTS (2,2′-azino-bis-(3-ethylbenozothiazonline-6-sulfonic acid)), 2,2-diphenyl-1-picrylhydrazyl (DPPH), p-nitrophenyl-α-D-glucopyranoside (pNPG), α-glucosidase, gallic acid, and rutin were purchased from Sigma-Aldrich (St. Louis, USA). Folin-ciocalteu reagent, acarbose, gallic acid, rutin, silicagel, dimethyl sulfoxide, and mEthanol were obtained from Merck (Darmstadt, Germany). Glibenclamide and streptozotocin were purchased from Hi-media (Mumbai). Ascorbic acid was from Scharlau (Scharlab). All of the other chemicals were of analytical grade.