Celltiterglo dye

Manufactured by Promega

Sourced in United Kingdom

CellTiterGlo®Dye is a cell viability assay that quantifies the amount of ATP present in metabolically active cells. It is a luminescent assay that provides a measurement of the number of viable cells in a sample.

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Lab products found in correlation

Topcount scintillation counter, by PerkinElmer (2 mentions) Chemotx plate, by Neuro Probe (1 mentions) Chemotx, by Neuro Probe (1 mentions)

2 protocols using celltiterglo dye

Chemotactic Responsiveness Assay with CellTiter-Glo

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Assays of chemotactic responsiveness were carried out as previously
described using 96-well ChemoTx® plates with 5μm pores
(Neuroprobe). Migrating cells were detected by the use of CellTiterGlo®
Dye (Promega) and resulting luminescence measured using a TopCount scintillation
counter (PerkinElmer). In all experiments, each data point was assayed in
duplicate. Data are reported as Chemotactic Indices, defined as the migratory
response to a particular stimulus divided by the migratory response to media
alone.

Puttur F., Denney L., Gregory L.G., Vuononvirta J., Oliver R., Entwistle L.J., Walker S.A., Headley M.B., McGhee E.J., Pease J.E., Krummel M.F., Carlin L.M, & Lloyd C.M. (2019). Pulmonary environmental cues drive group 2 innate lymphoid cell dynamics in mice and humans. Science immunology, 4(36), eaav7638.

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Chemotactic Responsiveness Assay

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Assays of chemotactic responsiveness were carried out as previously described (Vaidehi et al., 2009 (link)) using 96-well ChemoTx® plates with 5 μm pores (Neuroprobe, Gaithersburg, MD, USA). Migrating cells were detected by the use of CellTiter Glo® Dye (Promega, Southampton, UK) and resulting luminescence measured using a TopCount scintillation counter (PerkinElmer, Waltham, MA, USA). Basal migration of cells to buffer alone was subtracted from the resulting data, with individual results expressed as a percentage of the total cells applied to the filter. In all experiments, each data point was assayed in duplicate. In every experiment, cells transiently expressing WT CXCR3 were used as a positive control.

Nedjai B., Viney J.M., Li H., Hull C., Anderson C.A., Horie T., Horuk R., Vaidehi N, & Pease J.E. (2015). CXCR3 antagonist VUF10085 binds to an intrahelical site distinct from that of the broad spectrum antagonist TAK–779. British Journal of Pharmacology, 172(7), 1822-1833.

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