Gfp c myc ki

Mice were bred at Dana-Farber Cancer Institute in accordance with the guidelines of the Institutional Animal Care and Use Committee (IACUC). The following strains were used in this study, all on the C57BL/6 genetic background: C57BL6/J (CD45.2⁺; The Jackson Laboratory), B6.SJL (CD45.1⁺; The Jackson Laboratory), Rag2^−/−Il2rg^−/−(Taconic), Klra8−/− (Ly49H-deficient)⁶⁶ , IRE1^f/f67 , XBP1^{f/f11 (link)}, Myc^f/f (a generous gift from Dr. Ruoning Wang), Ncr1^iCre (a generous gift from Dr. Eric Vivier), ERAI (ER stress-activated indicator)^{19 (link)}, and GFP-c-Myc KI (here called Myc^GFP, the Jackson Laboratory). Myc^fsf/fsf on mixed B6.126 background was a generous gift from Dr. Rosaline Sears. IRE1^f/f Ncr1^iCre/WT (here called IRE1^NK), XBP1^f/f Ncr1^iCre/WT (XBP1^NK), Myc^f/+ Ncr1^iCre/WT (Myc^NK), Myc^fsf/+ Ncr1^iCre/WT (Myc^OE) and Myc^fsf/+ IRE1^f/f Ncr1^iCre/WT (Myc^OE IRE1^NK) animals and littermate controls were generated by breeding at Dana-Farber Cancer Institute. Recipient Klra8−/− (CD45.1⁺CD45.2⁺) mice were generated by crossing Klra8−/− (CD45.2⁺) to B6.SJL (CD45.1⁺). Experiments were conducted using age- and gender-matched mice in accordance with approved institutional protocols.