Mpo af3667

The following primary antibodies were used in this study: periostin (ab14041; Abcam, Cambridge, MA, USA), alpha-smooth muscle actin (α-SMA: ab5694; Abcam, Cambridge, MA, USA), and collagen type 1 α2 (LS-C343921-100; LsBio, Seattle, WA, USA) for immunohistochemistry (IHC); connective tissue growth factor (CTGF: LS-B3284-50; LsBio, Seattle, WA, USA), transforming growth factor-beta (TGF-β: MAB240-100; R&D, Minneapolis, MN, USA), myeloperoxidase (MPO: AF3667; R&D, Minneapolis, MN, USA), vascular endothelial growth factor (VEGF: BS2431; Bioworld Technology, Louis Park, MN, USA), and β-actin (sc-47778; Santa Cruz Biotechnology, Inc., Dallas, Texas, USA) for western blotting. The following secondary antibodies were used: mouse anti-rabbit IgG-horseradish peroxidase (IgG-HRP: sc-2357; Santa Cruz Biotechnology, Inc., Dallas, Texas, USA), mouse anti-goat IgG-HRP (sc-2354; Santa Cruz Biotechnology, Inc., Dallas, Texas, USA), and rabbit anti-mouse IgG-HRP (ab6728; Abcam, Cambridge, MA, USA).

Primary antibodies used for Western blotting (WB), immunofluorescence (IF) staining and flow cytometry (FC) were as follows with dilutions inticated in parenthesis: MPO, clone 8F4 (HyCult) (IF: 1:200); MPO, AF3667, R&D (1:400); MMP9, AF909, R&D (WB 1:1000); the anti-WASH (WB 1:1000) and anti-FAM21 (IF 1:200) antibodies were described before^{25 (link),28 (link)}. The anti-CD63 (clone NVG-2), anti-CD11b (clone M1/70) and anti-Ly6G (clone 1A8, 127610) (flow cytometry) antibodies were from Biolegend (FC 1:50). The anti-neutrophil elastase, Ab68672, was from Abcam (FC 1:50) and phalloidin was from Thermo Fischer. The anti-Rac1 antibody was from Proteintech (24072-1-AP) (WB 1:1000) and the anti-Rac1-GTP from NewEast Biosciences (26903) (IF 1:100); anti-RhoA was from Santa Cruz Biotechnology (SC-418) (WB 1:1000; IF 1:100), anti-Rab21 from Novus Biologicals (NBP1-81544) (IF 1:200) and anti-Arp2 (ab49674) (IF 1:200) was from Abcam. Myc-Tag (9B11) Mouse mAb #2276 (WB:1:1000). Anti-mCherry antibody Abcam, [EPR20579] (ab213511), WB, 1:1000.