Mice were anaesthetised with inhalant isoflurane (1–3%). A 50 μl amount of Griffonia (Bandeiraea) simplicifolia lectin 1 (BS1 lectin, 1 mg/ml, Vector Laboratories, Burlingame, CA, USA) was directly injected into the LV chamber. After 15 min, the heart tissues were harvested and fixed in 10% (vol./vol.) formalin and embedded and sectioned at 5 μm thickness. After processing, the sections were incubated with goat anti-BS1 lectin antibody (1:100; Vector Laboratories) and then stained with Alexa Fluor 594-conjugated rabbit anti-goat IgG (1:500, Invitrogen, Waltham, MA, USA). The fluorescent signals were quantified from at least eight random high-power fields for each section. The results were presented as capillary density per field. Blinding was carried out.
Goat anti bs1 lectin antibody
Manufactured by Vector Laboratories
Sourced in United States
Goat anti-BS1 lectin antibody is a primary antibody produced in goats that binds to the BS1 lectin. The BS1 lectin is a carbohydrate-binding protein found in various biological samples. This antibody can be used to detect and study the presence and distribution of the BS1 lectin in research applications.
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Lab products found in correlation
Alexa fluor 594 conjugated goat anti rabbit igg, by Thermo Fisher Scientific (1 mentions)
Bs1 lectin, by Vector Laboratories (1 mentions)
Anti α sma, by Santa Cruz Biotechnology (1 mentions)
Anti vimentin, by Abcam (1 mentions)
Alexa fluor 488 conjugated anti goat igg, by Jackson ImmunoResearch (1 mentions)
Anti cd68, by Abcam (1 mentions)
Anti cd31, by Abcam (1 mentions)
2 protocols using goat anti bs1 lectin antibody
1
Visualization of Cardiac Microvascularization
2
Comprehensive Immunofluorescent Staining Protocol
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Immunofluorescent staining was performed according to the standard protocol as previously reported [19 (link)]. Briefly, the heart sections were incubated with anti-vimentin, anti-CD68, anti-CD31 (Abcam), and anti-α-SMA (Santa Cruz) to detect fibroblasts, macrophages, and blood vessels. Then, FITC-conjugated anti-mouse IgG or anti-rabbit IgG was added and incubated for 1 h before observation. For detection of neovascularization, 1 mg/mL Griffonia (Bandeiraea) simplicifolia lectin 1 (BS1 lectin; Vector) was injected into the left ventricle via direct cardiac puncture 15 min before the sacrifice of mice. Slides were sequentially stained with goat anti-BS1 lectin antibody (Vector) and Alexa Fluor 488-conjugated anti-goat IgG (Jackson ImmunoResearch). Finally, the heart sections were mounted with DAPI-containing anti-fade medium and imaged.
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