Flouromount g

Manufactured by Thermo Fisher Scientific

Sourced in United States

Fluoromount G is a versatile aqueous-based mounting medium designed for use with fluorescent-labeled specimens. It is formulated to preserve the fluorescence intensity of a wide variety of fluorescent dyes and proteins, ensuring optimal signal retention during sample preparation and microscopy analysis.

Automatically generated - may contain errors

Lab products found in correlation

Alexa fluor 488, by Thermo Fisher Scientific (2 mentions) Fibronectin coated coverslips, by Merck Group (1 mentions) Axiovert 200, by Zeiss (1 mentions) Anti ym1, by STEMCELL (1 mentions) Rabbit anti actin, by Thermo Fisher Scientific (1 mentions) Rabbit anti il 4 receptor, by Santa Cruz Biotechnology (1 mentions) Anti iba1, by Fujifilm (1 mentions) Alexa fluor 488 phalloidin, by Thermo Fisher Scientific (1 mentions) Hematoxylin, by Merck Group (1 mentions) Horseradish peroxidase, by Thermo Fisher Scientific (1 mentions) Alexa fluor 488 or 546 conjugated secondary antibody, by Thermo Fisher Scientific (1 mentions) Surgipath micromount, by Leica (1 mentions) 3 3 diaminobenzidine (dab), by Agilent Technologies (1 mentions) Xylene, by Merck Group (1 mentions) No 1 cover glasses, by Avantor (1 mentions)

5 protocols using flouromount g

Visualizing Viral and Cellular Proteins

Check if the same lab product or an alternative is used in the 5 most similar protocols

Immunofluorescence assays of viral protein ICP4 were performed on cells grown on coverslips. Samples were fixed in 4% paraformaldehyde and incubated with the appropriate primary and secondary antibodies. DAPI (5 mg/ml) was added 10 min before the end of the procedure to visualize the nuclei. Cells were examined using a Zeiss Axiovert 200 fluorescence microscope. Images were captured by a Spot RT slider digital camera (Diagnostic) using MetaMorphTM imaging software, and processed using Adobe Photoshop CS4.
For confocal microscopy analysis of SAMHD1 subcellular localization, cells were grown on fibronectin-coated coverslips (20 µg/ml; Sigma) and fixed in 4% PFA. For SAMHD1 staining, blocking and staining solutions were composed of 0.2% Triton X-100, γ-globulin and TNB and appropriated primary and secondary antibodies. Samples were washed with TBS-Triton and mounted on coverslips with Flouromount-G (Thermo Fisher) containing DAPI (0.1 μM). Confocal images were obtained with an A1R + Nikon confocal microscopy attached to an inverted microscope (Eclipse Ti-E model, Nikon) with a Plan-Apocromatic 60X/1.4 oil immersion objective, using NIS Elements 4.40 acquisition software. Images were analysed with ImageJ.

Benayas B., Sastre I., López-Martín S., Oo A., Kim B., Bullido M.J., Aldudo J, & Yáñez-Mó M. (2020). Tetraspanin CD81 regulates HSV-1 infection. Medical Microbiology and Immunology, 209(4), 489-498.

+ Open protocol

+ Expand

Immunofluorescent Staining of Microglia

Check if the same lab product or an alternative is used in the 5 most similar protocols

To validate in vivo imaging data mice from each experimental group were euthanized by overdose of anesthetic. For the double-immunofluorescence analysis, mice were perfused with PBS (pH 7.5) followed by 40 mg/ml paraformaldehyde and incubated overnight in 200 mg/ml phosphate-buffered sucrose. The sections were blocked in 10% goat serum and then incubated overnight at room temperature using primary antibodies diluted in 5% goat serum/PBS1x+0.25% Triton X-100 (1:500 rabbit polyclonal anti-Iba1 (Wako), 1:500 anti-Gal-3 (ATCC), 1:250 rabbit anti IL-4 receptor (Santa Cruz), 1:500 rabbit anti CD11b (Serotech), 1:500 rabbit polyclonal anti-Ym1 (Stem Cell technologies) and 1:500 rabbit anti actin (Invitrogen). After washes in PBS, the sections were then incubated in corresponding fluorescent goat secondary antiserum for 2 h (1:500, Invitrogen), washed and mounted with Flouromount G (Thermofisher) (Rahimian et al., 2019b (link)). Alexa Fluor® 488 phalloidin (Thermo Fisher Scientific) was used to study microglia morphology (Leica CTR 500 microscope).

Rahimian R., Lalancette-Hébert M., Weng Y.C., Sato S, & Kriz J. (2020). Glucosamine-mediated immunomodulation after stroke is sexually dimorphic. Brain, Behavior, & Immunity - Health, 3, 100041.

+ Open protocol

+ Expand

Immunohistochemical Analysis of Mouse Brain

Check if the same lab product or an alternative is used in the 5 most similar protocols

Mice were anesthetized and then perfused with saline followed by 4% paraformaldehyde in PBS (PFA/PBS), via IV cardiac puncture. Brains were removed, fixed overnight in PFA/PBS, placed in 30% sucrose for 1–2 days, frozen in OCT, and cryosectioned at 30 μm. Sections were incubated in blocking buffer (10% normal goat serum in 0.3% triton in PBS) for 1 h, incubated with primary antibodies (see antibody list in Table 2) overnight at 4 °C, washed with PBS, incubated with secondary antibody (1:600) and Hoescht (1:10,000) for 1–2 h at RT, washed, mounted with Flouromount G (ThermoFisher, New Jersey, United States, cat. 00-4958-02), and topped with a glass cover. Stained sections were imaged with an epifluorescent microscope.

Quezada A., Ward C., Bader E.R., Zolotavin P., Altun E., Hong S., Killian N.J., Xie C., Batista-Brito R, & Hébert J.M. (2023). An In Vivo Platform for Rebuilding Functional Neocortical Tissue. Bioengineering, 10(2), 263.

+ Open protocol

+ Expand

Immunohistochemical Tissue Staining Protocol

Check if the same lab product or an alternative is used in the 5 most similar protocols

Skin samples for tissue staining were fixed with 4% paraformaldehyde in phosphate-buffered saline (PBS) at 4 °C for 1 day and then embedded in paraffin. The tissues were sectioned to a 6-μm thickness. Paraffin was removed by xylene (Sigma-Aldrich). Sections were soaked in descending alcohol. The tissue sections were boiled in sodium citrate (pH 6) for antigen retrieval, treated with 3.5% H₂O₂ (Sigma-Aldrich) in methanol, blocked using 5% goat serum for 1 h at room temperature, treated with the primary antibody overnight at 4 °C, and then incubated with the secondary antibody conjugated with either horseradish peroxidase (Invitrogen) or fluorescent dyes (Alexa Fluor 488- or 546-conjugated secondary antibodies) (Invitrogen) for 2 h at room temperature. For immunofluorescence staining, 4',6-diamidino-2-phenylindole (DAPI) was used as a nuclear counterstain. For immunohistochemistry, samples were further treated with 3,3'-diaminobenzidine (Dako, Glostrup Kommune, Denmark) and were then counterstained with hematoxylin (Millipore). The slides for immunohistochemistry were sealed with a coverslip using the Surgipath micromount (Leica Biosystems, Wetzlar, Germany). The slides for immunofluorescence staining were sealed using Flouromount-G (Invitrogen).

Hong Y.K., Lin Y.C., Cheng T.L., Lai C.H., Chang Y.H., Huang Y.L., Hung C.Y., Wu C.H., Hung K.S., Ku Y.C., Ho Y.T., Tang M.J., Lin S.W., Shi G.Y., McGrath J.A., Wu H.L, & Hsu C.K. (2024). TEM1/endosialin/CD248 promotes pathologic scarring and TGF-β activity through its receptor stability in dermal fibroblasts. Journal of Biomedical Science, 31, 12.

+ Open protocol

+ Expand

Measuring DNA Damage in Irradiated Cells

Cited 1 time

Check if the same lab product or an alternative is used in the 5 most similar protocols

RT112 cells were cultured on 10 mm No. 1 cover glasses (VWR) that had been sterilised with 70% ethanol and rinsed with PBS prior to ionising radiation. After incubation with bacterial supernatants and irradiation, cells were allowed to recover for indicated times prior to permeabilisation with 0.3% Triton X-100. Cells were fixed with ice cold 4% paraformaldehyde and blocked by incubation in 5% BSA, as described previousl y[84 (link)]. After incubation with primary γH2AX (mouse anti-phospho-Histone H2A.X (Ser139) IgG; clone JBW301, Millipore) and secondary (goat anti-mouse IgG, Alexa Fluor 488, ThermoFisher) antibodies, coverslips were mounted on microscopy slides using mounting reagent, Flouromount G, with DAPI (Invitrogen). Fluorescent foci were imaged using a Zeiss 710 confocal microscopy using either a 40X or 63X objective. All microscopy images were analysed with FIJI (ImageJ) software.

Then C.K., Paillas S., Moomin A., Misheva M.D., Moir R.A., Hay S.M., Bremner D., Roberts (nee Nellany) K.S., Smith E.E., Heidari Z., Sescu D., Wang X., Suárez-Bonnet A., Hay N., Murdoch S.L., Saito R., Collie-Duguid E.S., Richardson S., Priestnall S.L., Wilson J.M., Gurumurthy M., Royle J.S., Samuel L.M., Ramsay G., Vallis K.A., Foster K.R., McCullagh J.S, & Kiltie A.E. (2024). Dietary fibre supplementation enhances radiotherapy tumour control and alleviates intestinal radiation toxicity. Microbiome, 12, 89.

+ Open protocol

+ Expand

About PubCompare

Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.

We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.

However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.

Ready to get started?

Revolutionizing how scientists
search and build protocols!