Average particle size (APS) and polydispersity index (PdI) were determined using Zeta Plus Analyzer (Brookhaven Instruments Corporation, Holtsville, NY, USA) and followed by zeta potential measurement. Fresh empty NEs and Apig-loaded NEs were pipetted 1 mL and appropriately diluted in pure water to reach the test criteria. Hydrodynamic particle size and polydispersity index were obtained by 10 min of automatic cycle calculation and the zeta potential was measured by phase analysis light scattering combined with electrophoresis technique. At least three independent measurements were performed for each sample and reported as the mean±standard deviation. Besides, the storage stable days of NEs at 24.5 °C were determined by average particle size (APS) < 500 nm, polydispersity (PdI) <0.4, or no phase separation through optical observation.
Zeta plus analyzer
Manufactured by Brookhaven Instruments
Sourced in United States
The Zeta Plus Analyzer by Brookhaven Instruments is a device used for measuring the zeta potential and size of particles in a liquid suspension. It utilizes electrophoretic light scattering technology to determine the surface charge and hydrodynamic size of the particles.
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Lab products found in correlation
Zetasizer nano z, by Malvern Panalytical (1 mentions)
Ls 10, by Zeiss (1 mentions)
Item program, by Olympus (1 mentions)
Keenviewii, by Olympus (1 mentions)
Jem 2100, by JEOL (1 mentions)
Nano zs90, by Malvern Panalytical (1 mentions)
Jem 1011, by JEOL (1 mentions)
Jem arm200f, by JEOL (1 mentions)
Nicolet avatar 360 ftir, by Thermo Fisher Scientific (1 mentions)
Jem 2011, by JEOL (1 mentions)
Multimode afm, by Digital Instruments (1 mentions)
Nanoscope 4 controller, by Digital Instruments (1 mentions)
Mpp 11100, by Bruker (1 mentions)
Jem 1200ex microscope, by JEOL (1 mentions)
F 2700 fluorescence spectrophotometer, by Hitachi (1 mentions)
17 protocols using zeta plus analyzer
1
Physicochemical Characterization of Apig-Loaded Nanoemulsions
2
Characterization and Stability Evaluation of Cc Oil Nanoemulsions
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Average particle size (APS), PdI, and ZP of Cc oil-NEs were measured by Zeta Plus Analyzer (Brookhaven Instruments Corporation, Holtsville, NY, USA) based on the principles of dynamic light scattering for size and distribution and Doppler velocimetry (electrophoretic light scattering) for zeta potential. Before each measurement, the fresh samples were suitably diluted in pure water to avoid light interruption. The values of APS, PdI, and ZP (the mean ± standard deviation) were calculated from triplicate measurements per sample. Additionally, the NEs, with a volume of 20 mL, were stored at room temperature, approximately 25 °C. The storage stability the of NEs was evaluated based on the following criteria: APS < 500 nm, PdI < 0.4, or no optical phase separation observed.
3
Morphological Characterization of Nanoparticles
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Morphological examination of NP was performed by environmental scanning electron microscopy (ESEM) (Zeiss LS10, Germany). The samples were placed on aluminum stub, then analyzed at 10 kV acceleration voltage after gold sputtering (Sputter Coater Edwards S150A), under an argon atmosphere. Mean diameter and polydispersity index (PDI) of NPs were measured by using photon correlation spectroscopy (Zetasizer Nano ZS, Malvern Instruments, U.K.) at 25 °C and a scattering angle of 90° after dilution of samples with Milli-Q water. Each sample was measured in triplicate. The ζ potential values of the NP were detected with a Zeta Plus analyzer (Brookhaven, USA) at 25 °C. Each sample of NP was diluted with distilled water (1.0 mg/2.0 mL) and sonicated before measurement. All data were obtained with the average of three measurements.
4
Nanoparticle Characterization by SEM, DLS, and BET
5
Emulsion Particle Size and Viscosity
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The particle size distributions and polydispersity index (PDI) of emulsion were measured using a dynamic light scattering (DLS) by a Zeta Plus Analyzer (Brookhaven Instruments Corporation, Holtsville, NY, USA) before VSD. Each sample was diluted 20× and was measured independently in triplicate [3 (link)]. The viscosity was determined by Vibro viscometer SV-10/SV-100 (A&D, Tokyo, Japan). The viscometer was calibrated using a standard viscosity fluid with the known value of viscosity and density of 0.93 mPa.s at 23 °C using DI water. The sample was added to the disposable cup until 10 mL, then measured for 10 min.
6
Synthesis and Characterization of Silver Nanoparticles
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AgNPs were prepared by Nano Trade Company (Czech Republic). In brief, AgNO3 was dissolved in distilled water and NaBH4 added under constant magnetic stirring. Formation of AgNPs occurred rapidly upon addition of NaBH4 (Frankova et al.,2016 (link)). The AgNPs were characterized by ultraviolet-visible (UV-VIS) spectroscopy (from 200 nm to 800 nm) and transmission electron microscopy (TEM). The analysis was performed using a JEOL JEM 2011 transmission electron microscope at an accelerating voltage of 100 kV. Photographs were taken with a Morada or Keen View II digital camera and the iTEM program (SIS, Olympus). Zeta Plus analyzer (Brookhaven) was used to measure the zeta potential. The silver nanoparticles used in our study had an average size of approximately 10 nm (more than 50%).
7
Nanoparticle Characterization by TEM, DLS, and Zeta Potential
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The morphology of the nanoparticles was analyzed by transmission electron microscopy (JEM-2100; JEOL, Tokyo, Japan) and the hydrodynamic diameter was measured by dynamic light scattering (Nano ZS90; Malvern Instruments, Westborough, MA, USA). The zeta potential of these materials when dissolved in deionized water was determined using a Zeta Plus analyzer (Brookhaven Instruments, Holtsville, NY, USA).
8
Comprehensive Characterization of Nanobubbles
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The morphology of NBs was examined using a transmission electron microscope (TEM) (JEM-1011, JEOL, Inc., Peabody, MA, USA). The mean diameter and polydispersity of NBs were measured using photon correlation spectroscopy (90 Plus particle sizer, Brookhaven Instruments Corp). Measurements were performed at 25°C with a laser wavelength of 660 nm at an angle of 90°. Bubble size was measured by diluting a sample formulation 1:1000, with PBS at pH 7.4. The bubble size was reported as the number average. The zeta potential of NBs was determined by using a Zeta Plus Analyzer (90Plus/BI-MAS, Brookhaven, Holtsville, NY, USA). Measurements were performed at 25°C and each sample was run using 7 repetitions and the average was reported.
9
Zeta Potential Analysis of Nanoparticles
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Zeta potential of the NPs was measured at 25°C with a Zeta Plus analyzer (Brookhaven Instruments, Holtsville, NY, USA). The samples were diluted with distilled water and sonicated for several minutes before measurement. Data were obtained by the averaging of three measurements.
10
Characterization of SiO2 nanoparticles
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For hydrodynamic size and ζ-potential, 1 mg of SiO2-NPs was suspended in 1 mL of Basal Medium Eagle (BME) or Dulbecco’s Modified Eagle Medium (DMEM) cell culture, and both were supplemented with 10% of fetal bovine serum (FBS) and 1% of antibiotic-antifungal GibcoTM, (Thermo Fisher Scientific, Waltham, MA, USA). Then, the suspension was sonicated at 60 Hz for 30 min. Immediately, the stocks were diluted in a ratio of 1:100 in cell culture medium for hydrodynamic size measured by dynamic light scattering and for polydispersion index and ζ-potential using a Zeta Plus Analyzer (Brookhaven Instruments, Holtsville, NY, USA).
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