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Mouse anti flag mab clone m2

Manufactured by Merck Group
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The Mouse anti-FLAG mAb clone M2 is a monoclonal antibody that specifically recognizes the FLAG epitope tag. It can be used for the detection and purification of FLAG-tagged recombinant proteins in various laboratory applications.

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Lab products found in correlation

Mouse anti cmyc mab 9e10, by Thermo Fisher Scientific (4 mentions) Anti ha, by Thermo Fisher Scientific (2 mentions) Rat anti ha mab clone 3f10, by Roche (2 mentions) Mouse anti resa mab clone 28 234, by Merck Group (2 mentions) Anti ha, by Merck Group (2 mentions) Anti flag 8h8l17, by Thermo Fisher Scientific (2 mentions) Mouse anti exp2 mab clone 7, by Thermo Fisher Scientific (2 mentions) Anti sbp138, by Thermo Fisher Scientific (2 mentions) Anti pfhad139, by Thermo Fisher Scientific (2 mentions) Anti plasmodium aldolase ab38905, by Abcam (2 mentions) Anti ha sg77, by Thermo Fisher Scientific (2 mentions)

Close spelling variants of this product

Mouse anti-FLAG (clone M2, Mouse Flag mAb (M2, Anti-FLAG (clone M2, MAb-Flag (M2, Mouse anti-FLAG mAb FLAG (clone M2, Anti-FLAG M2 Anti-FLAG mAb FLAG M2 MAb-Flag

6 protocols using mouse anti flag mab clone m2

1

Comprehensive Antibody Characterization for Malaria Research

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for IFA and Western blot: rabbit polyclonal anti-HA (Life Technologies) (IFA: 1:100); rabbit polyclonal anti-HA (Sigma) (WB 1:1000); rat anti-HA mAb clone 3F10 (Roche) (IFA: 1:100, WB: 1:1000); mouse anti-FLAG mAb clone M2 (Sigma) (IFA: 1:100, WB 1:500); mouse anti-EXP2 mAb clone 7.733 (link) (IFA: 1:1000, WB: 1:1000); mouse anti-HRP2 mAb 2G1214 (link) (IFA 1:1000, WB: 1:500); mouse anti-RESA mAb clone 28/234 (IFA: 1:1000, WB: 1:1000); rabbit polyclonal anti-BiP35 (link) (WB: 1:1000); rabbit polyclonal anti-REX136 (link) (IFA: 1:1000); mouse polyclonal anti-REX215 (link) (IFA: 1:500); mouse polyclonal anti-REX315 (link) (IFA: 1:500); rabbit polyclonal anti-SBP121 (link) (IFA: 1:500); mouse polyclonal anti-MSRP622 (link) (IFA: 1:500); mouse polyclonal anti-PfGECO27 (link) (IFA: 1:300); rabbit polyclonal anti-Pfs1637 (link) (IFA: 1:500); rabbit polyclonal anti-PfEMP1 ATS38 (link) (IFA: 1:200); mouse polyclonal anti-CLAG328 (link) (IFA: 1:300); rabbit polyclonal anti-PM2 antibody 73739 (link) (WB 1:2000); rabbit polyclonal anti-KAHRP16 (link) (IFA: 1:500); rabbit polyclonal anti-SERP40 (link) (IFA: 1:500, WB: 1:1000).
Beck J.R., Muralidharan V., Oksman A, & Goldberg D.E. (2014). HSP101/PTEX mediates export of diverse malaria effector proteins into the host erythrocyte. Nature, 511(7511), 592-595.
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2

Antibody Dilutions for IFA and Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for IFA and western blot at the indicated dilutions: rabbit polyclonal anti-HA SG77 (ThermoFisher) (WB: 1:1000); mouse anti-FLAG mAb clone M2 (Sigma) (IFA: 1:500, WB 1:500); rabbit mAb anti-FLAG 8H8L17 (ThermoFisher) (WB: 1:500); mouse anti-EXP2 mAb clone 7.736 (WB: 1:1000); mouse anti-HRP2 mAb 2G1237 (IFA 1:500); rabbit polyclonal anti-SBP138 (IFA: 1:500); rabbit polyclonal anti-PfHAD139 (WB: 1:500); mouse anti-cMYC mAb 9E10 (ThermoFisher) (WB: 1:300); rabbit polyclonal anti-Plasmodium Aldolase ab38905 (Abcam) (IFA: 1:1000).
Garten M., Nasamu A.S., Niles J.C., Zimmerberg J., Goldberg D.E, & Beck J.R. (2018). EXP2 is a nutrient-permeable channel in the vacuolar membrane of Plasmodium and is essential for protein export via PTEX. Nature microbiology, 3(10), 1090-1098.
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3

Protein Immunofluorescence and Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following primary antibodies were used for IFA and western blot: mouse anti-FLAG mAb clone M2 (Sigma) (IFA: 1:500, WB 1:500); rabbit polyclonal anti-SBP1 [Ref 59 (link)] (IFA: 1:500); mouse anti-cMYC mAb 9E10 (ThermoFisher) (WB: 1:300).
Ho C.M., Beck J.R., Lai M., Cui Y., Goldberg D.E., Egea P.F, & Hong Zhou Z. (2018). Malaria Parasite Translocon Structure and Mechanism of Effector Export. Nature, 561(7721), 70-75.
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4

Comprehensive Antibody Characterization for Malaria Research

Cited 2 times
Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for IFA and Western blot: rabbit polyclonal anti-HA (Life Technologies) (IFA: 1:100); rabbit polyclonal anti-HA (Sigma) (WB 1:1000); rat anti-HA mAb clone 3F10 (Roche) (IFA: 1:100, WB: 1:1000); mouse anti-FLAG mAb clone M2 (Sigma) (IFA: 1:100, WB 1:500); mouse anti-EXP2 mAb clone 7.733 (link) (IFA: 1:1000, WB: 1:1000); mouse anti-HRP2 mAb 2G1214 (link) (IFA 1:1000, WB: 1:500); mouse anti-RESA mAb clone 28/234 (IFA: 1:1000, WB: 1:1000); rabbit polyclonal anti-BiP35 (link) (WB: 1:1000); rabbit polyclonal anti-REX136 (link) (IFA: 1:1000); mouse polyclonal anti-REX215 (link) (IFA: 1:500); mouse polyclonal anti-REX315 (link) (IFA: 1:500); rabbit polyclonal anti-SBP121 (link) (IFA: 1:500); mouse polyclonal anti-MSRP622 (link) (IFA: 1:500); mouse polyclonal anti-PfGECO27 (link) (IFA: 1:300); rabbit polyclonal anti-Pfs1637 (link) (IFA: 1:500); rabbit polyclonal anti-PfEMP1 ATS38 (link) (IFA: 1:200); mouse polyclonal anti-CLAG328 (link) (IFA: 1:300); rabbit polyclonal anti-PM2 antibody 73739 (link) (WB 1:2000); rabbit polyclonal anti-KAHRP16 (link) (IFA: 1:500); rabbit polyclonal anti-SERP40 (link) (IFA: 1:500, WB: 1:1000).
Beck J.R., Muralidharan V., Oksman A, & Goldberg D.E. (2014). HSP101/PTEX mediates export of diverse malaria effector proteins into the host erythrocyte. Nature, 511(7511), 592-595.
+ Open protocol
+ Expand
5

Antibody Dilutions for IFA and Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following antibodies were used for IFA and western blot at the indicated dilutions: rabbit polyclonal anti-HA SG77 (ThermoFisher) (WB: 1:1000); mouse anti-FLAG mAb clone M2 (Sigma) (IFA: 1:500, WB 1:500); rabbit mAb anti-FLAG 8H8L17 (ThermoFisher) (WB: 1:500); mouse anti-EXP2 mAb clone 7.736 (WB: 1:1000); mouse anti-HRP2 mAb 2G1237 (IFA 1:500); rabbit polyclonal anti-SBP138 (IFA: 1:500); rabbit polyclonal anti-PfHAD139 (WB: 1:500); mouse anti-cMYC mAb 9E10 (ThermoFisher) (WB: 1:300); rabbit polyclonal anti-Plasmodium Aldolase ab38905 (Abcam) (IFA: 1:1000).
Garten M., Nasamu A.S., Niles J.C., Zimmerberg J., Goldberg D.E, & Beck J.R. (2018). EXP2 is a nutrient-permeable channel in the vacuolar membrane of Plasmodium and is essential for protein export via PTEX. Nature microbiology, 3(10), 1090-1098.
+ Open protocol
+ Expand
6

Protein Immunofluorescence and Western Blot

Check if the same lab product or an alternative is used in the 5 most similar protocols
The following primary antibodies were used for IFA and western blot: mouse anti-FLAG mAb clone M2 (Sigma) (IFA: 1:500, WB 1:500); rabbit polyclonal anti-SBP1 [Ref 59 (link)] (IFA: 1:500); mouse anti-cMYC mAb 9E10 (ThermoFisher) (WB: 1:300).
Ho C.M., Beck J.R., Lai M., Cui Y., Goldberg D.E., Egea P.F, & Hong Zhou Z. (2018). Malaria Parasite Translocon Structure and Mechanism of Effector Export. Nature, 561(7721), 70-75.
+ Open protocol
+ Expand

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