V. unguiculata (Burpee black-eyed pea no. 5 plants) were seeded in 3–1/4” square pots (Greenhouse Megastore) using Pro Mix BX soil (Greenhouse Megastore) and maintained in Conviron A1000 Reach-in growth chambers (Conviron) at 16/8 hour day/night cycles at 25 °C/22 °C and 60% relative humidity. When the plants were 10 days old, the primary leaves were mechanically inoculated with 40 μL (0.1 mg mL−1) of native or inactivated CPMV (see above); the leaves were lightly dusted with carborundum (Thermo Fisher Scientific) to promote mechanical lesions on the leaves enabling the virus infection. 10 plants were used per treatment arm; in addition, 10 plants were not treated. Leaves were imaged and harvested 10 days post-inoculation and stored at −80 °C until further processing.
A1000 reach in growth chambers
Manufactured by Conviron
The Conviron A1000 is a reach-in growth chamber designed for controlled environmental conditions. It provides precise control of temperature, humidity, and lighting to support plant and other biological research.
Automatically generated - may contain errors
Lab products found in correlation
2 protocols using a1000 reach in growth chambers
1
Pea Virus Infection Assay
2
Pea Virus Inoculation Protocol
Check if the same lab product or an alternative is used in the 5 most similar protocols
V. unguiculata (Burpee black-eyed pea no. 5 plants) were seeded in 3–1/4′′ square pots (Greenhouse Megastore) using Pro Mix BX soil (Greenhouse Megastore) and maintained in Conviron A1000 Reach-in growth chambers (Conviron) at 16/8 hour day/night cycles at 25 °C/22 °C and 60% relative humidity. When the plants were 10 days old, the primary leaves were mechanically inoculated with 40 μL (0.1 mg mL−1) of native or inactivated CPMV (see above); the leaves were lightly dusted with carborundum (Thermo Fisher Scientific) to promote mechanical lesions on the leaves enabling the virus infection. 10 plants were used per treatment arm; in addition, 10 plants were not treated. Leaves were imaged and harvested 10 days post-inoculation and stored at −80 °C until further processing.
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V. unguiculata (Burpee black-eyed pea no. 5 plants) were seeded in 3–1/4′′ square pots (Greenhouse Megastore) using Pro Mix BX soil (Greenhouse Megastore) and maintained in Conviron A1000 Reach-in growth chambers (Conviron) at 16/8 hour day/night cycles at 25 °C/22 °C and 60% relative humidity. When the plants were 10 days old, the primary leaves were mechanically inoculated with 40 μL (0.1 mg mL−1) of native or inactivated CPMV (see above); the leaves were lightly dusted with carborundum (Thermo Fisher Scientific) to promote mechanical lesions on the leaves enabling the virus infection. 10 plants were used per treatment arm; in addition, 10 plants were not treated. Leaves were imaged and harvested 10 days post-inoculation and stored at −80 °C until further processing.
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