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4 protocols using anti abl

1

Antibody Reagents for Cell Analysis

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Unless stated otherwise, all chemicals were purchased from Sigma. Antibodies were obtained from the following sources: anti-ABL, anti-TAZ (BD Pharmingen), anti-RUNX2 (MBL International) and anti-Actin (Santa Cruz Biotechnologies). Halt™ Protease and Phosphatase Inhibitor Cocktail was from Thermo Fisher Scientific.
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2

Antibody-based Protein Signaling Analysis

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Unless stated otherwise, all chemicals were purchased from Sigma. Antibodies were obtained from the following sources: anti-pABL (Y245) (Cell Signaling Technology), anti-ABL (BD Pharmingen), anti-Flag M2 (Sigma), anti-Actin (Santa Cruz Biotechnologies), anti-RUNX2 (MBL International) and anti-pTyr (4G10) (EMD Millipore) antibodies. Halt™ Protease and Phosphatase Inhibitor Cocktail was from Thermo Fisher Scientific.
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3

Autophagy Assays in Cell Lines

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RPMI 1640 medium, fetal calf serum (FCS), phosphate buffered saline (PBS), were from Invitrogen. Trypan blue and the antibody against LC3 were from Sigma (St Quentin Fallavier, France). The following antibodies : ATG7 was from Cell Signalling (Danvers, USA), and Hsp60 was from Santa Cruz (Bergheimer, Germany). Annexin-V-APC was from Beckman coulter (Villepinte, France). Ki-67 antibody, control isotype and anti-Abl were purchased from BD (Becton Dickinson, France). PIK-III and SBI-0206965 were purchased from Selleck chemicals (Houston, USA).
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4

Investigating Cellular Signaling Pathways

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Unless stated otherwise, all chemicals were purchased from Sigma. Antibodies were obtained from the following sources: anti-ABL, anti-TAZ (BD Pharmingen), anti-RUNX2 (MBL International) and anti-Actin (Santa Cruz Biotechnologies). Halt™ Protease and Phosphatase Inhibitor Cocktail was from Thermo Fisher Scienti c.
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