MIC plates were made daily using the broth microdilution method with the standard 2-fold dilution series [81 (link)]. LB was used as the growth medium for all experiments (1% tryptone, 0.5% yeast extract, 1% NaCl). Antibiotics tested include piperacillin sodium, tobramycin, and ciprofloxacin HCl (Sigma). Aliquots of 1 mg/ml and 10 mg/ml antibiotic stocks were made by diluting the antibiotic powders in LB and were stored at −20°C. New frozen drug aliquots were used on a daily basis.
Ciprofloxacin hcl
Manufactured by Merck Group
Sourced in Germany
Ciprofloxacin HCl is a chemical compound used in various laboratory applications. It is a synthetic antibiotic that belongs to the fluoroquinolone class. Ciprofloxacin HCl is commonly used as a reference standard and in analytical procedures.
Automatically generated - may contain errors
Lab products found in correlation
Amphotericin b, by Thermo Fisher Scientific (2 mentions)
Dopamine, by Merck Group (1 mentions)
Azobisisobutyronitrile, by Merck Group (1 mentions)
Ethylene glycol dimethacrylate, by Merck Group (1 mentions)
Levofloxacin, by Merck Group (1 mentions)
2 hydroxyethyl methacrylate 2 hema, by Merck Group (1 mentions)
Tryptic soy broth tsb, by Merck Group (1 mentions)
Ampicillin, by Merck Group (1 mentions)
Ciprofloxacin, by Merck Group (1 mentions)
Nitrofurantoin, by Merck Group (1 mentions)
P aeruginosa pa01, by American Type Culture Collection (1 mentions)
Multi walled carbon nanotubes mwcnt, by Merck Group (1 mentions)
Tetrahydrofuran, by Merck Group (1 mentions)
Dibutyl phthalate, by Merck Group (1 mentions)
6 protocols using ciprofloxacin hcl
1
Broth Microdilution Antibiotic Testing
2
Early Human Tissue Procurement
3
Nephrite-Based Drug Delivery System
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Nephrite was obtained from Sujenara Co. (Seoul, Korea). Dopamine, ciprofloxacin HCl (CIP), levofloxacin (LEVO), ethylene glycol dimethacrylate (EGDMA), azobisisobutyronitrile (AIBN), 2-hydroxyethyl methacrylate (2-HEMA) and tryptic soy broth (TSB) were purchased from Sigma-Aldrich Chem. Co. Ltd. (St. Louis, MO, USA). Dialysis membranes with 500~1000 g/mol and 8000 g/mol of molecular weight cut-off (MWCO) were purchased from Spectrum Lab., Inc. (New Brunswick, NJ, USA). All of the organic solvents, such as dichloromethane (DCM), dimethylsulfoxide (DMSO) and ethyl alcohol (EtOH), were used as an ultrapure grade.
4
Antibiotic Susceptibility Profiling of Uropathogenic E. coli and P. aeruginosa
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E.coli UTI89 and E.coli CFT073 were obtained from Harry Mobley (University of Michigan); both strains have been sequenced. E.coli UTI89 is a prototypic cystitis isolate (1 (link)) and E. coli CFT073 is a prototypical UPEC isolate cultured from blood and urine of a patient with pyelonephritis (10 (link)). P.aeruginosa-Boston 41501 was purchased from American Type Culture Collection (Manassas, VA), and P.aeruginosa-PA01 was obtained from Kevin McIver; only PA01 has been sequenced. Antibiotics (Ampicillin, Ciprofloxacin and Nitrofurantoin) were purchased from Sigma-Aldrich (St. Louis, MO) and used at the following concentration ranges: Ciprofloxacin HCl (Cipro: 200 ng/mL and 2 μg/mL), Nitrofurantoin (Nitro: 20 μg/mL and 40 μg/mL), and Ampicillin (Amp: 50 μg/mL). DMSO was used as a solvent to dilute all the antibiotics. The final concentration of DMSO was 0.1%. Biofilm formation was not affected at this low concentration of DMSO.
5
Electrochemical Sensor Fabrication and Analysis
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ERY (USP) Reference Standard with purity 99.5% (CAS No. 114078), ciprofloxacin HCl monohydrate (98.5%), tetracycline HCl (USP Reference Standard, 98.0% purity), azithromycin reference substance (98.2% of purity), methacrylic acid (MAA; 99%, with 250 ppm of MEHQ inhibitor), 2,2’-azobisisobutyronitrile (AIBN), ethylene glycol dimethacrylate (EGDMA; 98%, with 90–110 ppm of MEHQ inhibitor), tetrahydrofuran (THF ≥ 99.9%, anhydrous), dibutyl phthalate (DPB), potassium tetrakis[3,5-bis(trifluoromethyl)phenyl] borate (TFPB), tetradecyl ammonium bromide (TDAB), potassium chloride, nanoparticles water dispersion of poly(3,4-ethylenedioxythiophene) (PEDOT), acetonitrile (analytical grade), and dimethyl sulfoxide (DMSO), and multiwalled carbon nanotubes (MWCNTs, 10 ± 1 nm O.D × 4.5 ± 0.5 nm I.D × 36 μm L) were purchased from Sigma Aldrich, Germany.
A paste of MWCNTs (70% w/w) in mineral oil (Fluka, USA) was added to copper electrode surface. Polyvinyl chloride (PVC) of relatively high molecular mass purchased from Fluka, Germany. Milk products were purchased from a local Egyptian supermarket. Buffers of 3–10 pH range were prepared following Britton and Robinson method [41 (link)].
A paste of MWCNTs (70% w/w) in mineral oil (Fluka, USA) was added to copper electrode surface. Polyvinyl chloride (PVC) of relatively high molecular mass purchased from Fluka, Germany. Milk products were purchased from a local Egyptian supermarket. Buffers of 3–10 pH range were prepared following Britton and Robinson method [41 (link)].
6
Early Human Tissue Procurement
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Placenta and fetal liver were de-identified, discarded material obtained from elective termination of first and second trimester pregnancies following informed consent. As these tissues are discarded material with no personal identifiers, this research does not constitute research on Human Subjects. This protocol was reviewed by the UCLA IRB committee, who determined such studies can be performed without further IRB review. Specimen age for this study is denoted as developmental age, two weeks less than gestational age, and was determined by ultrasound or estimated by the date of the last menstrual period. Tissues were harvested into PBS 5% FBS (Hyclone), Ciprofloxacin HCl (10 ng/mL, Sigma), amphotericin B (2.50 μg/mL, Invitrogen), 1% penicillin/streptomycin, transported on ice and processed the same day.
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