Ghrelin peptide

Manufactured by Phoenix Pharmaceuticals

Sourced in United States

Ghrelin peptide is a small protein molecule that functions as a growth hormone secretagogue. It stimulates the release of growth hormone from the pituitary gland.

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3 protocols using ghrelin peptide

Ghrelin Signaling Pathway Regulation

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Rapamycin and DMSO were obtained from Sigma-Aldrich (St Louis, MO, USA). Ghrelin peptide was purchased from Phoenix Pharmaceuticals Inc. (Burlingame, CA). Rabbit anti-phospho-mTOR (ser235/236), rabbit anti-mTOR, rabbit anti-phospho-S6 (ser235/236), rabbit anti-S6, rabbit anti-phospho-AKT (ser473), rabbit anti-AKT, rabbit anti-insulin, rabbit anti-phospho-Smad2 (ser465/467), mouse anti-Smad2, rabbit anti-phospho-Smad3 (ser423/425), mouse anti-GAPDH, and mouse anti-β-actin antibodies were obtained from Cell Signaling Technology (Beverly, MA). Rabbit anti-αSMA and mouse anti-ghrelin antibodies were purchased from Abcam (Cambridge, UK). IRDye-conjugated affinity purified anti-rabbit, anti-mouse IgGs were purchased from Rockland (Gilbertsville, PA). Goat anti-rabbit fluoresceinisothiocyanate-conjugated IgG, goat anti-mouse Texas Red-conjugated IgG, and goat anti-insulin A (C-12) antibody were purchased from Santa Cruz Biotechnology Inc. (Santa Cruz, CA). Trizol reagent and the reverse transcription (RT) system were from Promega Inc. (Madison, WI).

Yu R., Li Z., Liu S., Huwatibieke B., Li Y., Yin Y, & Zhang W. (2018). Activation of mTORC1 signaling in gastric X/A-like cells induces spontaneous pancreatic fibrosis and derangement of glucose metabolism by reducing ghrelin production. EBioMedicine, 36, 304-315.

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Ghrelin Peptide Signaling Pathway

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The ghrelin peptide was purchased from Phoenix Pharmaceuticals, Inc. (Burlingame, CA, USA). Dexamethasone was purchased from Sigma-Aldrich (St. Louis, MO, USA). Mouse anti-β-actin and rabbit anti-S6 and anti-p-S6 were obtained from Cell Signaling Technology (Beverly, MA, USA). Rabbit anti-GHSR1a and rabbit anti-Clock were purchased from Santa Cruz Inc. (Santa Cruz, CA, USA). Rabbit anti-Per2 was from MBL Inc. (Medical & Biological Laboratories, Nagoya, Japan).

Wang Q., Yin Y, & Zhang W. (2018). Ghrelin Restores the Disruption of the Circadian Clock in Steatotic Liver. International Journal of Molecular Sciences, 19(10), 3134.

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Ghrelin Receptor Signaling Pathway

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The ghrelin peptide was purchased from Phoenix Pharmaceuticals, Inc. (Burlingame, CA). Mouse antiβ-actin, rabbit anti-PCNA, rabbit anti-cyclinD1, rabbit anti-p27, rabbit anti-β-catenin and rabbit anti-pGSK3β (Ser9) were obtained from Cell Signaling Technology (Beverly, MA). Rabbit anti-GHS-R1a was purchased from Santa Cruze Inc. (Santa Cruze, CA).

Wang Q., Zheng M., Yin Y, & Zhang W. (2018). Ghrelin Stimulates Hepatocyte Proliferation via Regulating Cell Cycle Through GSK3β/Β-Catenin Signaling Pathway. Cellular physiology and biochemistry : international journal of experimental cellular physiology, biochemistry, and pharmacology, 50(5).

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