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2 protocols using acenaphthenol

1

Cytochrome P450 enzyme assay protocol

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Acenaphthenol, benzyloxyresorufin, bicinchoninic acid (BCA) assay kit, cytochrome c, NADH, NADPH, NADP+, 7-ethoxyresorufine, menadione, 7-methoxyresorufine, β-naphthoflavone, p-nitrophenyl sulfate, resorufin, R-sulforaphane, 3′-phosphoadenosin-5′-phosphate, and UDP-glucuronic acid were purchased from Sigma-Aldrich (Prague, Czech Republic). Midazolam was obtained from Toronto Research Chemicals (North York, ON, Canada). All other chemicals used were of HPLC or analytical grade. For real-time Polymerase Chain Reaction (PCR) analyses, ProtoScript® II Reverse Transcriptase was purchased from NEB (Ipswich, UK), TriReagent from Biotech (Prague, Czech Republic), and the qPCRCore kit for SYBR Green I from Eurogentec (Seraing, Belgium).
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2

Sesquiterpenes and Xenobiotic Metabolism

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Sesquiterpenes cis-nerolidol (CNER), trans-nerolidol (TNER), farnesol (FAR), and other chemicals benzyloxyresorufin, ethoxyresorufin, resorufin, coumarin, 7-ethoxy-4-(trifluoromethyl)coumarin, S-mephenytoin, diclofenac, bufuralol, testosterone, midazolam, chlorzoxazone, menadione, 1-chloro-2,4-dinitrobenzene, p-nitrophenol, p-nitrophenylsulphate, 2-napthol, 3’-phosphoadenosine-5’-phosphate, acenaphthenol, reduced glutathione, UDP-glucuronic acid, cytochrome c, NADH, NADP and NADPH, were purchased from Sigma-Aldrich (Prague, Czech Republic). All other chemicals used were of HPLC or analytical grade.
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