Pramipexole

Manufactured by Bio-Techne

Pramipexole is a synthetic compound that acts as a dopamine agonist. It is commonly used in various research applications to study dopamine-related processes and pathways.

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Lab products found in correlation

Protease inhibitor cocktail, by Thermo Fisher Scientific (2 mentions) Hygromycin, by Merck Group (1 mentions) Fluphenazine, by Merck Group (1 mentions) Tetracycline, by Merck Group (1 mentions) 3h sch23390, by PerkinElmer (1 mentions) Gtpγs, by Merck Group (1 mentions) Pd128907, by Bio-Techne (1 mentions) Lysis buffer, by Thermo Fisher Scientific (1 mentions) Phosphatase inhibitor cocktail, by Thermo Fisher Scientific (1 mentions) Blasticidin, by Merck Group (1 mentions) 3h methylspiperone, by PerkinElmer (1 mentions) 35s gtpγs, by PerkinElmer (1 mentions) Cell culture reagents, by Thermo Fisher Scientific (1 mentions) Ascorbic acid, by Merck Group (1 mentions) Quinpirole, by Bio-Techne (1 mentions) Skf81297, by Bio-Techne (1 mentions) L 745870, by Bio-Techne (1 mentions) Pd 168077, by Bio-Techne (1 mentions) D ap5, by Bio-Techne (1 mentions) Lisuride, by Bio-Techne (1 mentions)

3 protocols using pramipexole

Radioligand Binding Assays for Dopamine Receptors

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[³H]Methyl spiperone (85.5 Ci/mmol), [³H]SCH23390 (73.1 Ci/mmol), and [³⁵S]GTPγS (1250 Ci/mmol) were purchased from PerkinElmer (Boston, MA). Tetracycline, hygromycin, blasticidin, dopamine (DA), (+) butaclamol, fluphenazine, phenylmethylsulfonyl fluoride (PMSF), GDP, GTPγS, and ascorbic acid were obtained from Sigma-Aldrich (St. Louis, MO). G418 was obtained from Gemini Bio-Products (West Sacramento, CA); cell culture reagents were obtained from Invitrogen (Carlsbad, CA); and protease inhibitor cocktail, phosphatase inhibitor cocktail, and lysis buffer were obtained from Thermo Scientific (Rockford, IL). All test molecules (Table 1) were either synthesized in-house as described below or purchased from vendors. Asinex Corp. (Winston-Salem, NC): SK603, SK609, SK610, SK611; Enamine, Ltd. (Kiev, Ukraine): Compounds described in Lichtenberger patent²⁸ L1 and L2; Asiba Pharmatech, Inc. (Milltown, NJ): R- and S-enantiomers of SK609. DA and D2R/D3R agonists PD128907 and pramipexole were purchased from Tocris Bioscience (Bio-Techne, Inc., CA).

Xu W., Wang X., Tocker A.M., Huang P., Reith M.E., Liu-Chen L.Y., Smith AB I.I.I, & Kortagere S. (2016). Functional Characterization of a Novel Series of Biased Signaling Dopamine D3 Receptor Agonists. ACS chemical neuroscience, 8(3), 486-500.

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Dopamine Receptor Agonist and Antagonist Effects

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The DR1 agonist SKF-81297 (1 μM), the D2-like receptor agonist quinpirole (10 μM), the selective DR3 agonist pramipexole (10 μM), the DR4 agonist PD-168077 (200 nM), the selective DR4 antagonist L-745870 (500 nM), the dopamine (200 μM), the n-methy-d-aspartate (NMDA) receptor antagonist D-AP5 (50 μM) and CCH (5 μM) were purchased from Tocris Bioscience. Stock solutions, at thousand times the final concentration, were made up in water or in DMSO, and stored in individual aliquots at 20°C. Final solutions were prepared freshly on the day of the experiment. Drugs were applied to the recording ACSF 15 min after steady-state γ power was reached. The dead volume and bath volume was kept such that final concentration was reached in the bath in about 5 min.

Wang Y., Jin Y.K., Guo T.C., Li Z.R., Feng B.Y., Han J.H., Vreugdenhil M, & Lu C.B. (2022). Activation of Dopamine 4 Receptor Subtype Enhances Gamma Oscillations in Hippocampal Slices of Aged Mice. Frontiers in Aging Neuroscience, 14, 838803.

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Screening Compounds for Neurotransmitter Receptor Interactions

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All other screening compounds, except m-CPP (Sigma-Aldrich) were from Tocris Bioscience: Sumanirole (D₂ agonist), Spiperone (5-HT_2A serotonin and selective D₂-like dopamine receptor antagonist), Lisuride (D_2∼4 agonist), Pramipexole (D_2∼4 agonist), BW723C86 (5-HT_2B receptor agonist), m-CPP (Pan-serotonin agonist), Dihydrexidine (full agonist at the dopamine D1 and D5 receptors), Cabergoline (D_2∼4 agonist, possible prolactin inhibitor), DL-TBOA (EAAT blocker)

Liberante F.G., Pouryahya T., McMullin M.F., Zhang S.D, & Mills K.I. (2015). Identification and validation of the dopamine agonist bromocriptine as a novel therapy for high-risk myelodysplastic syndromes and secondary acute myeloid leukemia. Oncotarget, 7(6), 6609-6619.

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