Frozen sections (10 μm) were incubated with HADH antibody produced in rabbit (1/500; ThermoFisher Scientific). After washing, the sections were incubated with goat anti-rabbit IgG - Alexa Fluor® 568 conjugate (Thermo Fisher Scientific), followed by incubation with DAPI to stain nuclei. Images were acquired using a Multiphoton Laser Scanning Microscope (FV1000, Olympus).
Goat anti rabbit igg alexa fluor 568 conjugate
Manufactured by Thermo Fisher Scientific
Goat anti-rabbit IgG - Alexa Fluor® 568 conjugate is a secondary antibody reagent designed to detect and visualize rabbit primary antibodies in various immunoassay applications. It is a conjugate of goat-derived anti-rabbit immunoglobulin G (IgG) and the Alexa Fluor® 568 fluorescent dye.
Automatically generated - may contain errors
Lab products found in correlation
Goat anti mouse igg alexa fluor 488 conjugate, by Thermo Fisher Scientific (3 mentions)
Multiphoton laser scanning microscope fv1000, by Olympus (2 mentions)
Hdac1, by Cell Signaling Technology (1 mentions)
E cadherin, by Cell Signaling Technology (1 mentions)
Vimentin, by Cell Signaling Technology (1 mentions)
Annexin a1, by Cell Signaling Technology (1 mentions)
4 protocols using goat anti rabbit igg alexa fluor 568 conjugate
1
Immunohistochemical Localization of HADH
2
Immunofluorescent Staining of Muscle Stem Cells
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Frozen sections (10 μm) were incubated with mouse anti-MyoD antibody (Thermo Fisher Scientific Cat# MA1-41017 RRID:AB_2282434 ) or mouse anti-myogenin (DSHB Cat# F5D RRID:AB_2146602 ), mouse anti-Pax7 antibody (DSHB Cat# pax7 RRID:AB_528428 ), and rabbit anti-laminin antibody (Sigma-Aldrich Cat# L9393 RRID:AB_477163 ). After washing, the sections were incubated with goat anti-mouse IgG-Alexa Fluor® 488 conjugate (Thermo Fisher Scientific Cat# A-11001 RRID:AB_2534069 ) or goat anti-rabbit IgG-Alexa Fluor® 568 conjugate (Thermo Fisher Scientific Cat# A11011 RRID:AB_10584650 ). The sections were finally incubated with DAPI to stain nuclei.
3
Immunofluorescence Staining of Cell Markers
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Cells were fixed in 4% paraformaldehyde, and immunofluorescence staining was performed as described previously.11 (link) Antibodies against DAXX, E-cadherin, vimentin, CD44 and HDAC-1 were purchased from Cell Signaling (1/100). Antibodies against CD24 were purchased from Abcam (1/100). Goat anti-rabbit IgG—Alexa Fluor® 568 conjugate and goat anti-mouse IgG—Alexa Fluor®488 conjugate were purchased from Thermo Fisher Scientific (Waltham, MA). DAPI was added at the final step to reveal nuclei.
4
Immunofluorescence Staining of Frozen Tissue
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Frozen sections (10 μm) were incubated with Annexin A1 (Cell Signaling Technology), UQCRC1 (ThermoFisher Scientific) antibodies produced in rabbit, NNMT (Santa Cruz Biotechnology), Fibulin-5 (ThermoFisher Scientific) antibodies produced in mouse. After washing, the sections were incubated with goat anti-rabbit IgG - Alexa Fluor® 568 conjugate (Thermo Fisher Scientific), or goat anti-mouse IgG- Alexa Fluor® 488 conjugate, followed by incubation with DAPI to stain nuclei. Images were acquired using a Multiphoton Laser Scanning Microscope (FV1000, Olympus).
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