Hek293 cells
HEK293 cells are a widely used human cell line derived from human embryonic kidney cells. These cells are commonly used in cell biology research and biotechnology applications due to their ability to efficiently express recombinant proteins. HEK293 cells provide a reliable and well-established model system for various experimental purposes.
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18 protocols using hek293 cells
Interferon-γ Stimulation Assay in HEK293 Cells
Transient and Stable Transfection Assays
Cloning and Mutagenesis of Hepatic Transcription Factors
Quantifying SEAP Reporter Gene Activity
Quantifying VEGF-induced NFAT Activation
Transient Transfection of HEK293 Cells
Transfection and Proteasome Inhibition of KCNQ1
Stable Cell Lines for GPCR Studies
Quantitative Analysis of ADAM10 Expression
For quantitative PCR, cDNA generated from RNA extracts (QIAGEN RNeasy) of snap-frozen tumor samples was analysed using an iTaq Universal SYBR Green kit (Bio-Rad Laboratories, Hercules, CA, USA) and a RotorGene 3000 cycler (Corbett Research). Primers specific for human ADAM10 were used to determine expression by comparative CT (ΔΔCT), relative to the averaged expression of three housekeeping genes (GAPDH, Beta 2 microglobulin, and Eukaryotic translation initiation factor 4E).
Gs22a Cells for cAMP Reporting
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