MIAMI cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, rinsed 3× 5 minutes in PBS, and stored in sterile water at 4°C. Cells were permeabilized with 0.1% triton-x for 5-10 minutes at room temperature, blocked in 3% PBS-BSA for 30 minutes at room temperature and then stained with primary antibodies against GFP (AB3080, Millipore), Notch 2 (Ab8926, Abcam), Oct4 (MAB4401, Millipore), Lamin A (MAB3540, Millipore), Progerin (#05-1231, Millipore), Ki-67 (KI67-MM1-L-CE, Leica Biosystems, Buffalo Grove, IL,USA) for 1h at room temperature. Rinsed cells were incubated with fluorescently labeled secondary antibodies for 1h at room temperature. Coverslips were mounted on glass slides using DAPI mounting media. Cells not plated on coverslips were incubated with DAPI antibody for 15 minutes at room temperature prior to imaging.
Ki67 mm1 l ce
Manufactured by Leica
Sourced in United States
The KI67-MM1-L-CE is a laboratory equipment product designed for research purposes. It is a monoclonal antibody that binds to the Ki-67 protein, which is associated with cellular proliferation. The product is intended for in vitro diagnostic use and is CE-marked, indicating conformity with European Union regulations.
Automatically generated - may contain errors
Lab products found in correlation
Ab8926, by Abcam (1 mentions)
Mab3540, by Merck Group (1 mentions)
Mab4401, by Merck Group (1 mentions)
Ab3080, by Merck Group (1 mentions)
α sma, by Agilent Technologies (1 mentions)
Bond polymer refine detection, by Leica (1 mentions)
X tra adhesive slides, by Leica (1 mentions)
Bond rxm automated stainer, by Leica (1 mentions)
2 protocols using ki67 mm1 l ce
1
Immunofluorescence Staining of MIAMI Cells
2
Immunohistochemical analysis of FFPE tissue
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Formalin fixed paraffin embedded (FFPE) tissues were cut at 4 µm and mounted on X-tra™ Adhesive slides (Leica Biosystems UK Ltd, Milton Keynes, UK). Hematoxylin and eosin (H&E) stains were performed to confirm presence of tumor nodules on the CAM. Immunohistochemistry (IHC) was performed using the Bond RXm Automated Stainer with the Bond polymer refine detection systems in either red or brown, according to the manufacturers' instructions (Leica Biosystems UK Ltd). Tissue sections were stained for Melan-A (A103, 1;100, DAKO), Ki67 (MM1-L-CE, 1:200, Leica Biosystems) & α-SMA (1A4, 1:400, DAKO).
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