Lcq advantage trap mass spectrometer

A Jasco DIP-370 automatic polarimeter was used to determine the optical rotation. The UV spectra were measured on a UNICO UV-2102PCS spectrophotometer (Unico, Dayton, NJ, USA). The NMR spectra were determined using a Bruker AM-600 spectrometer (Bruck Biospin, Fallanden, Switzerland). The LCQ advantage trap mass spectrometer (Thermo Finnigan, San Jose, CA, USA) was equipped with an electrospray ionization (ESI) source, and high-resolution electrospray ionization mass spectra (HR-ESI-MS) were obtained using an Agilent 6530 Accurate-Mass Q-TOF LC/MS system. Column chromatography was performed using silica gel (Kieselgel 60, 70–230, and 230–400 mesh, Merck, Darmstadt, Germany) and YMC RP-18 resins, and thin layer chromatography (TLC) was performed using pre-coated silica-gel 60 F₂₅₄ and RP-18 F₂₅₄S plates (both 0.25 mm, Merck, Darmstadt, Germany). GC-MS data were obtained with an Clarus 600 GC equipped with a 600T mass selective detector and a 30 m (0.25 mm i.d., 0.25 μm film) HP-5 ms capillary column (Agilent, Wilmington, Germany). All isolation solvents were purchased from Daejung (Si Heung, Korea).

Optical rotations were determined using a Jasco DIP-370 automatic polarimeter. The nuclear magnetic resonance (NMR) spectra were recorded using a JEOL ECA 600 spectrometer (JEOL Ltd., Tokyo, Japan)(¹H, 600 MHz, ¹³C, 150 MHz), The licence controller qualification (LCQ) advantage trap mass spectrometer (Thermo Finnigan, San Jose, CA, USA) was equipped with an electrospray ionization (ESI) source, and high-resolution electrospray ionization mass spectra (HR-ESI-MS) were obtained using an Agilent 6530 Accurate-Mass quadrupole time-of-flight mass spectrometry (Q-TOF LC/MS) system. Column chromatography was performed using a silica gel (Kieselgel 60, 70–230, and 230–400 mesh, Merck, Darmstadt, Germany), YMC RP-18 resins, and thin layer chromatography (TLC) was performed using pre-coated silica-gel 60 F₂₅₄ and RP-18 F₂₅₄S plates (both 0.25 mm, Merck, Darmstadt, Germany).

Optical rotations were determined using a DIP-370 automatic polarimeter (Jasco, Easton, MD, USA). The FT-IR spectra were measured using a Jasco Report-100 infrared spectrometer; The NMR spectra were recorded using an ECA 600 spectrometer (¹H, 600 MHz; ¹³C, 150 MHz, JEOL, Tokyo, Japan). Mass spectra were recorded on an LCQ advantage trap mass spectrometer (Thermo Finnigan, San Jose, CA, USA) equipped with an electrospray ionization (ESI) source. High-resolution electrospray ionization mass spectra (HR-ESI-MS) were obtained using a 6530 Accurate-Mass Q-TOF LC/MS system (Agilent, Santa Clara, CA, USA). Column chromatography was performed using silica gel (Kieselgel 60, 70–230, and 230–400 mesh, Merck, Darmstadt, Germany) and YMC RP-18 resins (Merck), and thin layer chromatography (TLC) was performed using pre-coated silica-gel 60 F₂₅₄ and RP-18 F₂₅₄S plates (both 0.25 mm, Merck).