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Phosphate buffered saline solution pbs 10x

Manufactured by Merck Group

Phosphate buffered saline solution (PBS) (10X) is a commonly used buffer solution in biological research and laboratory applications. It is a concentrated form of the standard PBS solution, which is a physiologically balanced salt solution. The 10X PBS is designed to be diluted with deionized water to create the working strength 1X PBS solution. The 10X PBS solution maintains a stable pH and osmolarity, providing a suitable environment for various cell culture, immunoassay, and molecular biology procedures.

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3 protocols using phosphate buffered saline solution pbs 10x

1

Synthesis of Dextran-Based Magnetic Nanoparticles

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Iron (III) chloride hexahydrate (FeCl3·6H2O), iron (II) chloride (FeCl2·4H2O), 9 – 11 kDa dextran, epichlorohydrin (ECH) were obtained from Sigma Aldrich (St. Louis, MO). Ammonium hydroxide (NH4OH) was purchased from EMD Chemicals (Gibbstown, NJ). Phosphate buffered saline solution (PBS) (10X) was purchased from EMD Millipore (Billerica, MA). All materials were used as received.
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2

Synthesis of Multifunctional Nanoparticles

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Iron (III) chloride hexahydrate (FeCl3·6H2O), iron (II) chloride (FeCl2·4H2O), 9 – 11 kDa dextran, epichlorohydrin (ECH), cisplatin (CDDP), fibrinogen, di-methyl sulfoxide (DMSO), and thrombin were obtained from Sigma Aldrich (St. Louis, MO). Ammonium hydroxide (NH4OH) was purchased from EMD Chemicals (Gibbstown, NJ). N-[a-maimidoacetoxy] succinimide ester (AMAS) linker was obtained from Thermo Scientific (Rochester, NY). CREKA peptide conjugated with 5-FAM-aminohexanoic acid (FAM-CREKA) was custom ordered through Biomatik (Cambridge, Ontario). Fetal bovine serum (FBS) was purchased from Fisher Scientific (Florence, KY). Dulbecco's Modified Eagle Medium (DMEM), pen-strep, L-glutamine, Fungizone®, and sodium pyruvate were all purchased from Invitrogen (Grand Island, NY), and trypsin was purchased from American Type Culture Collection (ATCC, Manassas, VA). 190 proof ethanol was purchased from Pharmco-Aaper (Shelbyville, KY) and phosphate buffered saline solution (PBS) (10X) was purchased from EMD Millipore (Billerica, MA). All materials were used as received.
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3

Synthesis and Characterization of Dextran-based Nanoparticles

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Iron (III) chloride hexahydrate (FeCl3·6H2O), iron (II) chloride (FeCl2·4H2O), 9 – 11 kDa dextran, epichlorohydrin (ECH) were obtained from Sigma Aldrich (St. Louis, MO). Ammonium hydroxide (NH4OH) was purchased from EMD Chemicals (Gibbstown, NJ). Phosphate buffered saline solution (PBS) (10X) was purchased from EMD Millipore (Billerica, MA). The activation buffer 2-[N-morpholino] ethane sulfonic acid (MES), N-hydroxysulfosuccinimide (Sulfo-NHS) and 1-ethyl-3- [3-dimethylaminopropyl] carbodiimide hydrochloride (EDC) were purchased from Thermo scientific (Rockford, IL). The TAT peptide was purchased custom from Biomatik. Phosphate buffered saline (PBS), Dulbecco’s Modified Eagle Medium (DMEM), pen-strep, L-glutamine, Fungizone®, sodium pyruvate, and calcein AM were obtained from Invitrogen (Frederick, MD). Acridine orange was purchased from Nexcelom Bioscience (Lawrence, MA) and A549 lung carcinoma and trypsin were purchased from American Type Culture Collection (ATCC, Manassas, VA). All materials were used as received.
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