515 high performance liquid chromatography pump

Manufactured by Waters Corporation

The 515 high-performance liquid chromatography pump from Waters Corporation is a precision instrument designed to deliver a consistent and accurate flow of solvents for liquid chromatography applications. It features a dual-piston design and advanced flow control technology to ensure reliable and reproducible results.

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Lab products found in correlation

717 plus autosampler, by Waters Corporation (2 mentions) Evo 18, by Zeiss (1 mentions) Escalab 250xi, by Thermo Fisher Scientific (1 mentions) Oca 20 instrument, by Dataphysics (1 mentions) Avance 3 600 nmr spectrometer, by Bruker (1 mentions) Spd 6av uv detector, by Shimadzu (1 mentions) Gpc system, by Waters Corporation (1 mentions)

Close spelling variants of this product

515 pump (26 citations)

3 protocols using 515 high performance liquid chromatography pump

Polymer Characterization by Multi-Technique Analysis

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Size exclusion chromatography (SEC)
was used to measure the polydispersity index (PDI) of all the polymers.
The SEC was equipped with a Waters 2414 refractive index detector
and a Waters 515 high-performance liquid chromatography pump. THF
was used as the eluent, and the flow rate is 1.0 mL/min. The molecular
weight and PDI were calculated using monodispersed polystyrene as
the calibration standard. The ¹H NMR spectra were recorded
on a Bruker AVANCE III 600 NMR spectrometer. SEM observations were
performed using Zeiss EVO 18 operating at 10 kV. XPS spectra were
obtained on a Thermo Fisher Scientific spectrometer (ESCALAB 250Xi)
with a micro-focused raster-scanned X-ray beam. The take-off angle
is 90°. FTIR spectra were recorded on a Bruker Vector 70 FTIR
spectrometer. CAs of water or oil droplets (5.0 μL) were measured
on a Data Physics OCA 20 instrument. Atleast five different points
on the samples were measured.

Xiao M., Huang Y., Xu A., Zhang T., Zhan C, & Hong L. (2019). On-Demand Oil–Water Separation by Environmentally Responsive Cotton Fabrics. ACS Omega, 4(7), 12333-12341.

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Protein Aggregation Analysis by HP-SEC

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High-performance size-exclusion chromatography (HP-SEC) was performed to analyze the aggregation state of the protein materials as supplied from the manufacturer. The protein materials were dissolved in PB (10 mM (pH 7.2)) (except for etanercept, which was diluted in placebo buffer (pH 6.3)) and filtered through a 0.2-μm filter before measurement.
The protein samples of LYS, CC, MYO, OVA, and BSA (50 μL; ∼1 mg/mL) were analyzed with a Discovery BIO Gel Filtration column (Sigma-Aldrich, St. Louis, MO). A 515 high-performance liquid chromatography pump and 717 Plus autosampler (Waters, Milford, MA) were operated at a flow rate of 0.5 mL/min. The mobile phase consisted of 100 mM sodium PB, 200 mM sodium chloride, and 0.05% (w/v) sodium azide at a pH of 7.2 and was filtered through a 0.2-μm filter before use. Chromatograms were recorded with an SPD-6AV UV detector (Shimadzu) at a wavelength of 280 nm.
For etanercept analysis (50 μL; ∼2 mg/mL), a Yarra SEC-2000 size-exclusion column (Phenomenex, Utrecht, the Netherlands) was used with the running buffer composed of 50 mM phosphate, 150 mM arginine, and 0.025% NaN₃ at pH 6.5. UV detection was performed at 280 nm.

Yu M., Silva T.C., van Opstal A., Romeijn S., Every H.A., Jiskoot W., Witkamp G.J, & Ottens M. (2019). The Investigation of Protein Diffusion via H-Cell Microfluidics. Biophysical Journal, 116(4), 595-609.

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GPC-based Purification of PCB Extracts

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A Waters Corporation GPC system equipped with a 515 high performance liquid chromatography pump, a 717 plus autosampler, a 2487 dual-wavelength absorbance detector, and a fraction collector was used for extract purification. The GPC system was fitted with two Envirogel columns in series (19 × 300 mm and 19 × 150 mm), preceded by a Phenogel 10 μm linear/mixed guard column (50 × 7.8 mm). The columns were conditioned with approximately 500 mL of methylene chloride before sample analysis.
To determine collection time windows, the instrument was calibrated using a volume of 50 μL from a solution of 100 μg/mL of Aroolor® 1016tl260 mixture. Metholene chloride was used as the eluting solvent (at specified by the column manufacturer), with a flow rate of 5 mL/min. PCB compounds were found to elute; from the columns between 13 and 20 min in approximately 40 mL of the eluent volume (Figure 2).
As extract fractions eluted from the GPC columns, each fraction was collected in a 50 mL Zymark sample concentration tube located in a fraction collector rack. Using a TurboVap II extract concentration instrument, each sample was blown down to approximately 0.5 mL and quantitatively transferred by glass pipette into a 5 mL, clear glass vial for the sulfuric acid wash.

Osemwengie L.I, & Morgan J. (2019). PCBs in Older Buildings: Measuring PCB Levels in Caulk and Window Glazing Materials in Older Buildings. Environments, 6(2), 15.

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