Blood samples (10 cc) were taken at the baseline and at the end of study at Firouzgar laboratory of Endocrine and Metabolism Centre after an overnight fast. FPG was assayed using a standard enzymatic method with commercial kits (Pars Azmun, Tehran, Iran). HbA1c levels were measured by DS5 device and ESCALON Reagent (REF:14-35) kit. Serum insulin levels were assayed by ELISA method (Beckman Coulter-France). The homeostasis model of assessment-insulin resistance (HOMA-IR) was determined according to the standard formula [10 (link)]. Serum high sensitivity C-reactive protein (hs-CRP) levels were assessed by the immunoturbidometery method with commercial kits (Pars Azmun, Tehran, Iran). Serum levels of total antioxidant capacity (TAC), superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were assayed by ELISA method (ZellBio GmbH, Germany).
Elisa method
Manufactured by ZellBio
Sourced in Germany
ELISA (Enzyme-Linked Immunosorbent Assay) is a widely used analytical technique for the detection and quantification of specific proteins or other molecules in a sample. The core function of the ELISA method is to use antibodies and color changes to identify and measure the target analyte.
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Lab products found in correlation
6 protocols using elisa method
1
Biomarkers Assessment in Fasting Blood
2
Plasma Cytokine Analysis in TAO
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We analyzed plasma samples from 60 male Caucasian patients from February 2016 through February 2019. TAO diagnosis was clinically confirmed according to Shionoya’s criteria with angiography confirmation.20 (link) To prevent bias regarding the influence of smoking on Th17-associated cytokines, 30 age- and smoking habit-matched male Caucasian controls were enrolled in this study. The clinical manifestations of the patients, along with complete blood count (CBC) data, were documented for each patient. IL-22, IL-23 and IL-17 were evaluated using the ELISA method (ZellBio GmbH, Ulm, Germany). This study has been approved by the ethical committee of Mashhad University of Medical Sciences and all patients and controls signed a written consent form before participating in this study (ethical code: MUMS-961500). The data were analyzed using Statistical Package for the Social Sciences (SPSS) version 11.5 (SPSS Inc., Chicago, IL, USA).
3
TGF-β1 and SIRT1 Levels in Kidney Tissue
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TGF-β1 and SIRT1 levels of kidney homogenized tissue were measured by ELISA method (Zellbio, Germany). In this case, the measurement was based on the reaction between the antigen and the antibody, and finally the adsorption was read at 450 nm. After placing the adsorption and concentration of standard solutions in Excel program, the standard curve was prepared. Concentration of the samples was determined based on the adsorption using the standard curve linear Eqs. [40 (link)–42 (link)].
4
Quantifying Renal Apoptosis Markers
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After animals were sacrificed, all the animals’ kidneys were separated and placed in cold physiological saline and then homogenized using a homogenizer and centrifuged for 10 min to prepare supernatant fluid at 1000 rpm. Bax and Bcl-2 levels in kidney tissue were measured using the ELISA method according to the kit instructions (ZellBio, Germany). The values of these indices were reported in ng/mg protein [63 (link), 64 (link)].
5
Evaluating Serum Biomarkers in IBD
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At first, and 12th week of study, the weight of each patient was measured in light weight clothing, with accuracy of 100 g and height measurement of each patient without shoes was performed by meters mounted on a wall with a 1 cm accuracy. Then the body mass index (BMI) was calculated. After 9–12 h of fasting, 5 cc blood samples were taken from each patient and their serum was kept in the − 80°c freezer until serum measurement analysis.
IBDQ-9 and SCCAIQ were filled out at the beginning and the end of study. Serum concentrations of TAC, TOS and 25- hydroxyl vitamin D (25-OHD) concentration were measured using ELISA method (ZellBio GmbH, Ulm, Germany) [14 (link), 23 (link), 26 (link), 27 (link)].
IBDQ-9 and SCCAIQ were filled out at the beginning and the end of study. Serum concentrations of TAC, TOS and 25- hydroxyl vitamin D (25-OHD) concentration were measured using ELISA method (ZellBio GmbH, Ulm, Germany) [14 (link), 23 (link), 26 (link), 27 (link)].
6
Serum Biomarkers Assessment in Rats
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Serum Ca, P, ALP, and 25(OH)D were checked for all the rats. On day 22 of the study, serum Ca (mg/dL), P (mg/dL), and ALP (Iu/L) were evaluated via the colorimetric assay
with the AutoAnalyzer (Biosystems, S.A., Spain). Serum 25(OH)D (ng/mL) was measured via the ELISA method (ZellBio GmbH, Germany) with a 3.3% intra-assay coefficient of
variation and a 5.1% inter-assay coefficient of variation.
with the AutoAnalyzer (Biosystems, S.A., Spain). Serum 25(OH)D (ng/mL) was measured via the ELISA method (ZellBio GmbH, Germany) with a 3.3% intra-assay coefficient of
variation and a 5.1% inter-assay coefficient of variation.
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