Ru bpy 32

HER-2 protein (ab60866) and HER-2 antibody (Ab, ab214275) were purchased from Abcam company. The solution of HER-2 protein was provided by dissolving of the protein (250 mg/mL) in deionized water (DI). All the standard solutions used for calibration curve were provided by dilution of the source protein solution with DI. The 20 µg/mL of HER-2 Ab was used in all experiments.
All employed reagents were of high-pure grade and applied without any additional purification. Graphene oxide (GO) solution (2 mg/mL) and [Ru(bpy) 3 ] 2+ were purchased from sigma Aldrich Company. Chitosan (CS) powder, tripropylamine (TPrA), acetic acid, sulfuric acid, nitric acid, K 3 [Fe(CN) 6 ], KCl and EDC/NHS were acquired from Merck Company. HER-2 monoclonal antibody and its recombinant protein were obtained from Abcam Company. Phosphate buffer solutions (PBS) were prepared in room temperature and containing KCl (0.201 g/L), NaCl (8.000 g/L), Na 2 HPO 4 (3.581 g/L), KH 2 PO 4 (0.231 g/L), pH = 7.4. The other pH values were prepared by addition of 3 N solutions of NaOH and HCl. Chitosan solution was prepared by dissolving 0.1 mg chitosan powder in 10 mL acetic acid/water solution (1% v/v). In order to prepare the [Ru(bpy) 3 ] 2+ solution, 10 mg [Ru(bpy) 3 ] 2+ powder was dissolved in 2 mL DI.