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Anti tyro3

Manufactured by Cell Signaling Technology
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Anti-TYRO3 is a primary antibody that specifically recognizes the TYRO3 protein. TYRO3 is a receptor tyrosine kinase that plays a role in various cellular processes. The antibody can be used for the detection and analysis of TYRO3 in different experimental applications.

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Lab products found in correlation

Anti gfp, by GeneTex (3 mentions) Anti caspase 3, by Cell Signaling Technology (2 mentions) Anti myc tag, by Cell Signaling Technology (1 mentions) μ slide, by Ibidi (1 mentions) Foxm1, by Cell Signaling Technology (1 mentions) Aurora b, by Cell Signaling Technology (1 mentions) Survivin, by Cell Signaling Technology (1 mentions) Hrp linked anti mouse igg, by Cell Signaling Technology (1 mentions) Caspase 8, by Cell Signaling Technology (1 mentions) Cyclin d1, by Cell Signaling Technology (1 mentions) Aurora a, by Cell Signaling Technology (1 mentions) Phospho prb ser780, by Cell Signaling Technology (1 mentions) Mertk, by Cell Signaling Technology (1 mentions) Anti α tubulin, by Merck Group (1 mentions) C myc, by Cell Signaling Technology (1 mentions) Cleaved parp, by Cell Signaling Technology (1 mentions) Cleaved caspase 3, by Cell Signaling Technology (1 mentions) Anti β actin, by Merck Group (1 mentions) Pngase f, by New England Biolabs (1 mentions) Anti zeb1, by GeneTex (1 mentions)

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TYRO3 (7 citations)

5 protocols using anti tyro3

1

Immunofluorescence Imaging of TYRO3 in HCT116 Cells

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HCT116 cells or transiently transfected HCT116 cells were cultured on μ-slides VI0.4 (ibidi) and fixed in 4% paraformaldehyde. After washing with PBS containing 0.3% Triton X-100, cells were blocked with 5% goat serum before the cells were stained with anti-TYRO3 (Cell Signaling Technology, Origene, Abnova, Sigma), anti–Myc-Tag (Cell Signaling Technology), F-actin (Cayman), and anti-GFP (GeneTex) and counterstained with 4′,6-diamidino-2-phenylindole (DAPI) or Hoechst. Fluorescence was captured using a laser confocal microscope (Olympus FV1000).
Hsu P.L., Chien C.W., Tang Y.A., Lin B.W., Lin S.C., Lin Y.S., Chen S.Y., Sun H.S, & Tsai S.J. (2023). Targeting BRD3 eradicates nuclear TYRO3-induced colorectal cancer metastasis. Science Advances, 9(15), eade3422.
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2

Immunoblotting Analysis of Cellular Proteins

Cited 1 time
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Cell lysates were prepared and immunoblotting was carried out as previously described.13 (link) Anti-TYRO3 (Ref. 5585), AXL (Ref. 8661), MERTK (Ref. 4319), caspase-8 (Ref. 9746), cleaved caspase-3 (Ref. 9664), cleaved PARP (Ref. 5625), FOXM1 (Ref. 5436), pRB (Ref. 9309), phospho-pRB Ser780 (Ref. 9307), CYCLIN D1 (Ref. 2926), AURORA A (Ref. 14475), AURORA B (Ref. 3094), SURVIVIN (Ref. 2808), c-MYC (Ref. 9402), anti-mouse IgG, HRP-linked (Ref. 7076) and anti-rabbit IgG, HRP-linked (Ref. 7074) antibodies were purchased from Cell Signaling Technology (Ozyme, Montigny-le-Bretonneux, France). Anti-α-TUBULIN (Ref. T6199) and anti-β-ACTIN (Ref. A2228) antibodies were obtained from Sigma-Aldrich. For peptide-N-glycosidase F (PNGaseF), we digested 10 µg of protein with PNGaseF, according to the manufacturer’s instructions (New England Biolabs, Evry, France).
Dufour F., Silina L., Neyret-Kahn H., Moreno-Vega A., Krucker C., Karboul N., Dorland-Galliot M., Maillé P., Chapeaublanc E., Allory Y., Stransky N., Haegel H., Menguy T., Duong V., Radvanyi F, & Bernard-Pierrot I. (2019). TYRO3 as a molecular target for growth inhibition and apoptosis induction in bladder cancer. British Journal of Cancer, 120(5), 555-564.
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3

Western Blotting of Cell Signaling

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Western blotting was done following the standard protocols. Polyvinylidene difluoride membranes were labeled with anti–caspase-3 (Cell Signaling), anti-TYRO3 (Cell Signaling), anti-GFP (GeneTex), anti–MMP-2 (GeneTex), anti-lamin A/C (GeneTex), anti-BRD3 (Proteintech, Abnova), anti-BRD2 (Cell Signaling), anti-SNAI1 (Novus), anti-SLUG (GeneTex), anti-ZEB1 (GeneTex), anti–E-cadherin (Proteintech), or anti–β-actin (GeneTex) antibodies. Densitometry was done using ImageJ program from at least three independent experiments.
Hsu P.L., Chien C.W., Tang Y.A., Lin B.W., Lin S.C., Lin Y.S., Chen S.Y., Sun H.S, & Tsai S.J. (2023). Targeting BRD3 eradicates nuclear TYRO3-induced colorectal cancer metastasis. Science Advances, 9(15), eade3422.
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4

Immunohistochemical Analysis of TYRO3

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Tissues were fixed with 4% paraformaldehyde, and 6-μm paraffin sections of tissue were subjected to hematoxylin and eosin staining following standard procedures. For immunohistochemical staining, after antigen retrieval, tissue sections were incubated with anti–C-terminal of TYRO3 antibodies (Origene) and then secondary antibodies and then visualized with aminoethyl carbazole. For immunofluorescent staining, sections of the cecum, lymph node, and liver tissue were incubated with the antibodies as indicated, including anti-TYRO3 (Cell Signaling Technology), anti-GFP (GeneTex), anti-CD31 (BD Biosciences, Bioss), anti-CD4 (Abclonal), and anti–MMP-2 (Proteintech).
Hsu P.L., Chien C.W., Tang Y.A., Lin B.W., Lin S.C., Lin Y.S., Chen S.Y., Sun H.S, & Tsai S.J. (2023). Targeting BRD3 eradicates nuclear TYRO3-induced colorectal cancer metastasis. Science Advances, 9(15), eade3422.
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5

Antibody Panel for Signaling Pathway Analysis

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The following antibodies were utilized per manufacturers’ recommendations: rabbit monoclonal anti-phospho-Stat3 (Tyr705, D3A7, 9145), anti-Stat3 (79D7, 4904), anti-PARP (46D11), anti-Caspase-3 (9662), anti-cleaved Caspase 3 (Asp175, 5A1E, 9664), anti-Tyro3 (D38C6, 5585), anti-phospho-Akt (Ser473, D9E, 4060), anti-PDGFRβ (28E1, 3169), anti-Akt (11E7, 4685), anti-cyclin D1 (92G2, 2978), and anti-Vinculin (E1E9V, 13,901) from Cell Signaling (Danvers, MA); rabbit monoclonal anti-PDGF Receptor beta (Y92, ab32570) from Abcam (Cambridge, MA); mouse monoclonal β-actin (A1978), and anti-phospho-Erk 1/2 (AW39R) from Sigma Aldrich (Millipore Sigma, St. Louis, MO). Crizotinib was purchased from Sigma Aldrich and LC Laboratories (Woburn, MA) and sunitinib was purchased from Selleckchem (Houston, TX).
Quinn C.H., Beierle A.M., Williams A.P., Marayati R., Bownes L.V., Markert H.R., Aye J.M., Stewart J.E., Mroczek-Musulman E., Crossman D.K., Yoon K.J, & Beierle E.A. (2021). Downregulation of PDGFRß Signaling Overcomes Crizotinib Resistance in a TYRO3 and ALK Mutated Neuroendocrine-Like Tumor. Translational Oncology, 14(7), 101099.
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