Confluent monolayers were labeled for two hours with SPY650-FastAct (Cytoskeleton Inc) by adding 1x of the probe to growth medium. Immediately prior to imaging, DAPT (10 μM) was added to the culture medium. For the epithelial ducts, 1x SPY650-FastAct (Cytoskeleton Inc) was applied for four hours prior to imaging. All images were acquired on a Yokogawa CSU-X1 spinning disk confocal on a Nikon Ti-E microscope equipped with an imaging chamber equilibrated to 37 °C in 5% CO2 humidified air.
Spy650 fastact
Manufactured by Cytoskeleton
The SPY650-FastAct is a high-performance fluorescence microscopy system designed for rapid live-cell imaging. It features a patented fast-acting magnetic-based autofocus system that maintains precise focus during time-lapse experiments. The system is capable of capturing images at high speeds and can be used in a wide range of applications, including cell biology, neuroscience, and developmental biology research.
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Lab products found in correlation
2 protocols using spy650 fastact
1
Labeling Confluent Monolayers for Confocal Imaging
2
Live-cell Fluorescent Protein Labeling
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Cells were labeled for 2 h with SPY650-FastAct (Cytoskeleton, Inc.) by adding 1× of the probe to the growth medium. Immediately prior to imaging, DAPT (10 µM) was added to the culture medium. For the MCF10A epithelial ducts, 1× SPY650-FastAct (Cytoskeleton, Inc.) was applied for 4 h prior to imaging. All images were acquired on a Yokogawa CSU-X1 spinning disk confocal on a Nikon Ti-E microscope equipped with an imaging chamber equilibrated to 37°C in 5% CO2 humidified air.
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