Tris buffer

Manufactured by AppliChem

Sourced in United States, Germany

Tris buffer is a widely used buffer solution in biochemistry and molecular biology. It is a buffering agent that helps maintain a stable pH environment for various biological processes and reactions. Tris buffer is composed of tris(hydroxymethyl)aminomethane and is typically used to maintain a pH range between 7.0 and 9.0.

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TRIS/EDTA buffer (2 citations)

3 protocols using tris buffer

Nanoparticle Synthesis and Antibacterial Assay

Cited 1 time

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Cadmium chloride (CdCl₂), 3-mercaptoundecanoic acid (MPA), sodium citrate dihydrate sodium borohydride (Na₂BH₄), l-ethyl-3-(3-(dimethylamino)propyl) carbodiimide (EDC), Triton X-100, 2′,7′-dichlorofluorescein (H₂DCF), sodium phosphate monohydrate, AlamarBlue and melittin were obtained from Sigma-Aldrich. Sodium hydroxide (NaOH) was obtained from Acros Organic. Sodium tellurite was obtained from Alfa Aeser. Tris buffer was obtained from AppliChem. Aqua (sterile water) was obtained from BBraun. Mueller Hinton Broth (MHB) was obtained from BD Difco. EDTA-blood was either drawn fresh every day of assay from an approved volunteer or purchased from Innovative Research, Inc. (Michigan, U.S.). E. coli (Strain ATCC25922) cells were purchased from the Leibniz-Institute DSMZ. Poly(oxanorbornenes) (PONs) were synthesized and characterized as reported previously (see SI for details).^{21 (link),23 (link)} An Ultrathin LED Light Panel (Neutral White) was purchased from Environmental Lights, San Diego, CA.

Williams D.N., Saar J.S., Bleicher V., Rau S., Lienkamp K, & Rosenzweig Z. (2020). Poly(oxanorbornene)-Coated CdTe Quantum Dots as Antibacterial Agents. ACS applied bio materials, 3(2), 1097-1104.

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Single Molecule Imaging Protocols

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Live cell imaging was performed in HBSS buffer (Biochrom, Berlin, Germany). For single molecule detection of fixed cells a standard STORM buffer was used according to Dempsey et al.^{48 (link)} and van de Linde et al.^{49 (link)}. 100 mM MEA (beta-mercaptoethylamine, pH 8.5, Sigma-Aldrich, St. Louis, Missouri, USA), 140 U catalase (Sigma-Aldrich, St. Louis, Missouri, USA, C3515) and 10 U glucose oxidase (Sigma-Aldrich, St. Louis, Missouri, USA, G0543) were added immediately before use into Tris-buffer (50 mM Tris, 10 mM NaCl (both AppliChem, Darmstadt, Germany), pH 8) supplemented with 10% (w/v) glucose (AppliChem, Darmstadt, Germany). Imaging was performed under oxygen exclusion conditions.

Babel L., Grunewald M., Lehn R., Langhans M, & Meckel T. (2017). Direct evidence for cell adhesion-mediated radioresistance (CAM-RR) on the level of individual integrin β1 clusters. Scientific Reports, 7, 3393.

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Potato Protein and Maltodextrin Characterization

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Maltodextrin, with a dextrose equivalent (DE) of 2 and a molecular weight Mw of ~124 kDa (MD DE2) (ElianeTM MD2 by Avebe, Veendam, The Netherlands), maltodextrin DE 21 (Glucidex^® 21D by Roquette, Lestrem, France) (MD DE21), and potato protein (PP) (Solanic^® 300 by Avebe, Veendam, The Netherlands), with a protein content of 91.16 ± 0.3 g/100 g, were used. The chemicals ortho-phtaldialdehyde (OPA) (purity ≥ 99%), calcium chloride CaCl₂ (≥98%), trichloroacetic acid (≥99%), and sodium dodecyl sulfate (SDS) (≥99%) were purchased from Carl Roth GmbH & Co. KG, (Karlsruhe, Germany), along with TRIS-buffer from AppliChem GmbH (Darmstadt, Germany), and ethanol (≥99.8%), β-mercaptoethanol (≥98%), L-lysine, azocasein, bovine pancreatic trypsin, sodium hydroxide (NaOH), and hydrochloric acid (HCl) from Sigma-Aldrich Chemie GmbH (Steinheim, Germany), as well as 0.1 M sodium tetraborate buffer (pH 9.7) from Merck KGaA (Darmstadt, Germany).

Gibis M., Pribek F, & Weiss J. (2022). Effects of Electrospun Potato Protein–Maltodextrin Mixtures and Thermal Glycation on Trypsin Inhibitor Activity. Foods, 11(7), 918.

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