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Trypsin edta 0.25 trypsin edta

Manufactured by Thermo Fisher Scientific
Sourced in United States

Trypsin-EDTA (0.25% trypsin-EDTA) is a cell dissociation reagent used in cell culture applications. It is a solution containing 0.25% trypsin and 0.02% EDTA, which functions to break down the proteins that hold cells together, facilitating cell detachment from the culture surface.

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2 protocols using trypsin edta 0.25 trypsin edta

1

Cell Culture Media Preparation

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4-dimethylaminopyridine (DMAP), gelatine, and various other chemicals were purchased from Sigma-Aldrich (St. Louis, MO, USA). SU-8 2000 was purchased from MicroChem (Newton, MA, USA). Dulbecco’s Modified Eagle’s Medium (DMEM; Gibco, Grand Island, NE, USA), penicillin G-streptomycin sulphate (Life Technologies Corporation, Chicago, IL USA), and foetal bovine serum (FBS; Hangzhou Sijiqing Biological Engineering Materials Co., Ltd., Hangzhou, China) were used as the cell culture medium. Trypsin-EDTA (0.25% trypsin-EDTA) was obtained from Invitrogen (Carlsbad, CA, USA).
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2

Sodium Alginate-based Drug Delivery System

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CMC-Na (average Mw ≈ 10 kDa, degree of substitution = 0.7), minocycline hydrochloride (MH), and malachite green (MG) were purchased from Sigma-Aldrich (St. Louis, MO, USA). Na-Alg (40–90 mPa s in 1% aqueous solution, M/G ratio = 2.05, Mw = 16–34 kDa) was purchased from Fisher Scientific Co. (Pittsburgh, PA). Calcium chloride (CaCl2) was purchased from Macklin (Shanghai, China). Dulbecco's Modified Eagle's Medium (DMEM; Gibco, Grand Island), fetal bovine serum (FBS; Hangzhou Sijiqing Biological Engineering Materials Co., China) and penicillin G-streptomycin sulfate (Life Technologies Corporation, USA) were adopted as the cell culture medium. Trypsin-EDTA (0.25% trypsin-EDTA) was purchased from Invitrogen (Carlsbad, CA, USA).
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