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Mrc 5 feeder cells

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The MRC-5 feeder cells are a type of primary human diploid cell line derived from normal human lung fibroblast cells. These cells are commonly used as feeder layers to support the growth and maintenance of other cell lines, such as embryonic stem cells and induced pluripotent stem cells.

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Lab products found in correlation

96 well plate, by Corning (2 mentions) Fetal bovine serum (fbs), by Merck Group (2 mentions) Iscove modified dulbecco media (imdm), by Thermo Fisher Scientific (1 mentions)

Close spelling variants of this product

MRC-5 (361 citations) MRC-5 cells (74 citations)

3 protocols using mrc 5 feeder cells

1

Hybridoma Cell Cloning Protocol

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Fifty microliters of medium supplemented with 20% FBS (Sigma) and 3% to 5% Hybridoma Cloning Factor (Antibody Research, Inc.) was added to each well of a 96-well plate (Corning) containing 50 μL of MRC-5 feeder cells (ATCC CCL-171), giving a total volume of 100 μL/well. 100 μL of the hybridoma cell suspension was removed and transferred to the top left-hand well and mixed by pipetting. 1-in-2 dilutions were performed down the left-hand row of the plate (eight wells; seven dilution steps), followed by 1-in-2 dilutions across the plate using a multichannel pipette. Clones are visualized by microscopy after 10 to 12 days. Single-cell wells were selected for expansion.
Bu X., Juneja V.R., Reynolds C.G., Mahoney K.M., Bu M.T., McGuire K.A., Maleri S., Hua P., Zhu B., Klein S.R., Greenfield E.A., Armand P., Ritz J., Sharpe A.H, & Freeman G.J. (2021). Monitoring PD-1 Phosphorylation to Evaluate PD-1 Signaling during Antitumor Immune Responses. Cancer Immunology Research, 9(12), 1465-1475.
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2

Feeder Cell Preparation for FANCA Transfection

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Vials of MRC-5 feeder cells (ATCC, Manassas, VA, USA, #55-X) were resuspended in 27 mL of Iscove’s Modified Dulbecco’s Medium (IMDM, Gibco, #12440053) with HyClone Fetal Bovine Serum (20%, hFBS, R&D Systems, #S11550) and Penicillin–Streptomycin (1%, 10,000 U/mL, P/S, Invitrogen, #TMS-AB2-C) under normoxic conditions (21% O2, 5% CO2, 37 °C). Per T-25 flask, 3 mL of feeder cell suspension was plated for transfection of bulk FANCA c.3934 + 2T > C (rs771775516) peripheral blood mononuclear cells (PBMCs) 1–5 days post feeder cell thawing.
Sipe C.J., Kluesner M.G., Bingea S.P., Lahr W.S., Andrew A.A., Wang M., DeFeo A.P., Hinkel T.L., Laoharawee K., Wagner J.E., MacMillan M.L., Vercellotti G.M., Tolar J., Osborn M.J., McIvor R.S., Webber B.R, & Moriarity B.S. (2022). Correction of Fanconi Anemia Mutations Using Digital Genome Engineering. International Journal of Molecular Sciences, 23(15), 8416.
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3

Hybridoma Cell Cloning Optimization

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50 μL of medium supplemented with 20% FBS (Sigma) and 3%–5% Hybridoma Cloning Factor (Antibody Research, Inc.) was added to each well of a 96-well plate (Corning) containing 50 μL of MRC-5 feeder cells (ATCC CCL-171), giving a total volume of 100 μL/well. 100 μL of the hybridoma cell suspension was removed and transferred to the top left-hand well and mixed by pipetting. 1-in-2 dilutions were performed down the left-hand row of the plate (eight wells; seven dilution steps), followed by 1-in-2 dilutions across the plate using a multichannel pipette. Clones are visualized by microscopy after 10-12 days. Single-cell wells were selected for expansion.
Bu X., Juneja V.R., Reynolds C.G., Mahoney K.M., Bu M.T., McGuire K.A., Maleri S., Hua P., Zhu B., Klein S.R., Greenfield E.A., Armand P., Ritz J., Sharpe A.H, & Freeman G.J. (2021). Monitoring PD-1 Phosphorylation to Evaluate PD-1 Signaling during Antitumor Immune Responses. Cancer Immunology Research, 9(12), 1465-1475.
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