H9c2 cardiomyocyte extract (5 × 105 cells) was subjected to immunoblot analysis as previously described [46 (link),64 (link)], using an anti-Cd38 polyclonal antibody (Santa Cruz Biotechnology, Santa Cruz, CA, USA) raised against a peptide fragment of mouse Cd38 (residues 279–301 in [46 (link)]), anti-Ryr2 polyclonal antibody (PeproTech, Canbury, NJ, USA) [67 (link)], anti-Fkbp12.6 monoclonal antibody (Santa Cruz Biotechnology) raised against the amino acids 38–108 of human/rat/mouse FKBP12.6, anti-Pten monoclonal antibody raised against the full-length human PTEN protein (Abcam, Cambridge, UK), and anti-β-actin monoclonal antibody (Sigma, St. Louis, MO, USA) raised against Ac-Asp-Asp-Asp-Ile-Ala-Ala-Leu-Val-Ile-Asp-Asn-Gly-Ser-Gly-Lys. A SNAP id® 2.0 Protein Detection System (Merck Millipore, Burlington, MA, USA) was used for the analysis. The band intensities were analyzed using ImageJ software (National Institute of Health, Bethesda, MD, USA), as previously described [35 (link),46 (link),64 (link),82 (link),83 (link)].
Anti pten monoclonal antibody
Manufactured by Abcam
Sourced in United Kingdom, United States
Anti-Pten monoclonal antibody is a laboratory reagent used in research applications. It is designed to specifically recognize and bind to the Pten protein, which plays a role in cellular signaling and regulation. This antibody can be used to detect and study the Pten protein in various experimental systems.
Automatically generated - may contain errors
2 protocols using anti pten monoclonal antibody
1
Immunoblot Analysis of H9c2 Cardiomyocyte Proteins
2
Quantifying FASN and PTEN in Gastric Cancer
Check if the same lab product or an alternative is used in the 5 most similar protocols
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FASN expression was analyzed using human gastric cancer and normal tissue. Standard immunohistochemical procedures were carried out according to the manufacturer's protocol (Vector Laboratories, USA). Anti-FASN and anti-PTEN monoclonal antibody (Abcam, USA) was used as a primary antibody. Similar tissue sections were immunostained with normal IgG were used as negative controls. The specimens were examined and scored using a two-headed microscope. Staining intensity and the proportion of stained cells were semi-quantitatively determined. The intensity and percentage of positive cell scores were multiplied. All slides were scored by two observers and there are good concordances between two observers. Mean score for duplicate cores from each individual was calculated.
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