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Bmal1 antibody a302 616a

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The Bmal1 antibody (A302-616A) is a research-use-only product that specifically recognizes the BMAL1 protein. BMAL1 is a core component of the circadian clock mechanism and plays a crucial role in regulating circadian rhythms. This antibody can be used to detect and study the BMAL1 protein in various experimental applications.

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Lab products found in correlation

β actin mouse mab ac 74, by Merck Group (2 mentions) C myc antibody, by Cell Signaling Technology (2 mentions) Eif2α antibody, by Thermo Fisher Scientific (2 mentions)

Spelling variants of this product

BMAL1 (5 citations)

2 protocols using bmal1 antibody a302 616a

1

Western Blot Analysis of Circadian Clock

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissue was snap frozen on dry ice. 3.5 wt/vol lysis buffer was added and homogenized for 30 seconds or until completely homogenized. Lysates were sonicated and put on ice for 30 minutes with vortexing every 5 minutes. Then the lysate was cleared by 2X centrifugation at 25,000 rpm for 20 minutes. Equal concentration of total protein was subjected to western blot as described42 (link). Bmal1 antibody (A302-616A ) was purchased from Bethyl Laboratories Inc. Clock (D45B10) Rabbit mAb #5157, PERK (C33E10) Rabbit mAb #5683, Chop (L63F7) Mouse mAb #2895, ATF-4 (D4B8) Rabbit mAb #11815, phospho-eIF2α (Ser51) (119A11) Rabbit mAb #3597, Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb #2855, eIF4E Antibody #9742, IRE1α (14C10) Rabbit mAb #3294, eIF4A (F52) Antibody #2425, S6 Ribosomal Protein (5G10) Rabbit mAb #2217, Phospho-p70 S6 Kinase (Thr389) (1A5) Mouse mAb #9206, and c-Myc Antibody #9402, BCL6(D4I2V) Rabbit mAb #14895 were from Cell Signaling Technology. eIF2α antibody was purchased from ThermoFisher Scientific. β-actin Mouse mAb (AC-74) #5316 was purchased from Sigma. Detailed antibody information is available in supplementary Table 2.
Bu Y., Yoshida A., Chitnis N., Altman B.J., Tameire F., Oran A., Gennaro V., Armeson K.E., McMahon S., Wertheim G.B., Van Dang C., Ruggero D., Koumenis C., Fuchs S.Y, & Diehl J.A. (2017). A PERK-miR211 axis suppresses circadian regulators and protein synthesis to promote cancer cell survival. Nature cell biology, 20(1), 104-115.
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2

Western Blot Analysis of Circadian Clock

Cited 1 time
Check if the same lab product or an alternative is used in the 5 most similar protocols
Tissue was snap frozen on dry ice. 3.5 wt/vol lysis buffer was added and homogenized for 30 seconds or until completely homogenized. Lysates were sonicated and put on ice for 30 minutes with vortexing every 5 minutes. Then the lysate was cleared by 2X centrifugation at 25,000 rpm for 20 minutes. Equal concentration of total protein was subjected to western blot as described42 (link). Bmal1 antibody (A302-616A ) was purchased from Bethyl Laboratories Inc. Clock (D45B10) Rabbit mAb #5157, PERK (C33E10) Rabbit mAb #5683, Chop (L63F7) Mouse mAb #2895, ATF-4 (D4B8) Rabbit mAb #11815, phospho-eIF2α (Ser51) (119A11) Rabbit mAb #3597, Phospho-4E-BP1 (Thr37/46) (236B4) Rabbit mAb #2855, eIF4E Antibody #9742, IRE1α (14C10) Rabbit mAb #3294, eIF4A (F52) Antibody #2425, S6 Ribosomal Protein (5G10) Rabbit mAb #2217, Phospho-p70 S6 Kinase (Thr389) (1A5) Mouse mAb #9206, and c-Myc Antibody #9402, BCL6(D4I2V) Rabbit mAb #14895 were from Cell Signaling Technology. eIF2α antibody was purchased from ThermoFisher Scientific. β-actin Mouse mAb (AC-74) #5316 was purchased from Sigma. Detailed antibody information is available in supplementary Table 2.
Bu Y., Yoshida A., Chitnis N., Altman B.J., Tameire F., Oran A., Gennaro V., Armeson K.E., McMahon S., Wertheim G.B., Van Dang C., Ruggero D., Koumenis C., Fuchs S.Y, & Diehl J.A. (2017). A PERK-miR211 axis suppresses circadian regulators and protein synthesis to promote cancer cell survival. Nature cell biology, 20(1), 104-115.
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