Confocal microscopy analyses were performed on an Inverted Z.1 microscope (Zeiss, Germany) equipped with a Zeiss LSM 700 spectral confocal laser-scanning unit (Zeiss, Germany). Samples were excited with a 488 nm, 10 mW solid laser with emission split at 505 nm for YFP.
Inverted z 1 microscope
Manufactured by Zeiss
Sourced in Germany
The Inverted Z.1 microscope is a high-performance laboratory instrument designed for various imaging applications. It features an inverted optical configuration, allowing for convenient sample observation and manipulation. The Inverted Z.1 microscope provides users with a stable and precise platform for their research and analysis needs.
Automatically generated - may contain errors
Lab products found in correlation
5 protocols using inverted z 1 microscope
1
Confocal microscopy analyses of YFP
2
Confocal Microscopy of Floral Gynoecia
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
Confocal microscopy analyses were performed on an Inverted Z.1 microscope (Zeiss, Germany) equipped with Zeiss LSM 700 spectral confocal laser scanning unit (Zeiss, Germany), using a ZEN 2012 software. GFP and EYFP samples were both excited by a 488 nm, 10 mW solid laser with emission at 492–539 nm22 (link). For the lateral view of gynoecia, floral buds were dissected and mounted in water. For the top views of style region, gynoecia were dissected, mounted vertically in an agar dish and observed also using transmitting light (bright field)6 (link).
3
Cellular and Root Uptake of Cu6-PLGA NPs
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
In order to investigate the uptake of Cu6-PLGA NPs by cells and roots, differential interference contrast (DIC) and confocal microscopy were used. The analyses were performed on an inverted Z.1 microscope (Zeiss, Germany) equipped with a Zeiss LSM 700 spectral confocal laser scanning unit (Zeiss, Germany). For coumarin 6 detection, a 488 nm, 10 mW solid laser with an emission wavelength of 520 nm was employed, while for propidium iodide detection, a 555 nm, 10 mW solid laser with an emission wavelength split at 550 nm/LP 640 was employed.
4
Subcellular Localization of DRT111 and SF1
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The coding sequences of DRT111 and SF1 were cloned by Gateway technology in the pUGW2 and pUGW0 vectors (Nakagawa et al., 2007) to guarantee the downstream fusion of the C-terminal YFP region and upstream fusion of N-terminal YFP region, respectively. Primers are listed in Supplemental Table S2. Nicotiana tabacum leaf protoplasts were prepared and transfected according to Pedrazzini et al. (1997) . Forty micrograms of DNA for each construct was introduced in 1 3 10 6 protoplasts using PEG-mediated transfection. After a 16-h incubation in the dark at 25°C, the cells were imaged with an Inverted Z.1 microscope (Zeiss) equipped with a LSM 700 spectral confocal laser-scanning unit (Zeiss). Samples were excited with a 488-nm, 10-mW solid laser with emission split at 505 nm for YFP and excited with a 555-nm, 10-mW solid laser with emission split at 551 nm for chlorophyll detection.
5
Transient Expression of Fluorescent Fusion Proteins in Tobacco Leaf Protoplasts
Check if the same lab product or an alternative is used in the 5 most similar protocols
+ Expand
The CDS of DRT111 and SF1 were cloned by Gateway technology in the pUGW2 and pUGW0 vectors (Nakagawa et al., 2007) (link) to guarantee the downstream fusion of the C-terminal YFP region and upstream fusion of N-terminal YFP region, respectively. Primers are listed in Supplemental Table S2. Nicotiana tabacum leaf protoplasts were prepared and transfected according to Pedrazzini et al. (1997) (link). 40 µg of DNA for each construct was introduced in 1x10 6 protoplasts using PEGmediated transfection. Following 16h incubation in the dark at 25°C, the cells were imaged with an Inverted Z.1 microscope (Zeiss, Germany) equipped with a Zeiss LSM 700 spectral confocal laserscanning unit (Zeiss, Germany). Samples were excited with a 488 nm, 10 mW solid laser with preprint (which was not certified by peer review) is the author/funder. All rights reserved. No reuse allowed without permission.
The copyright holder for this this version posted February 9, 2020. ; https://doi.org/10.1101/2020.02.07.939421 doi: bioRxiv preprint emission split at 505 nm for YFP and excited with a 555 nm, 10 mW solid laser with emission split at 551 nm for chlorophyll detection
The copyright holder for this this version posted February 9, 2020. ; https://doi.org/10.1101/2020.02.07.939421 doi: bioRxiv preprint emission split at 505 nm for YFP and excited with a 555 nm, 10 mW solid laser with emission split at 551 nm for chlorophyll detection
About PubCompare
Our mission is to provide scientists with the largest repository of trustworthy protocols and intelligent analytical tools, thereby offering them extensive information to design robust protocols aimed at minimizing the risk of failures.
We believe that the most crucial aspect is to grant scientists access to a wide range of reliable sources and new useful tools that surpass human capabilities.
However, we trust in allowing scientists to determine how to construct their own protocols based on this information, as they are the experts in their field.
Ready to get started?
Sign up for free.
Registration takes 20 seconds.
Available from any computer
No download required
Revolutionizing how scientists
search and build protocols!