Thy 1 apc

All BM‐MNC experiments were performed using Poietics BM‐MNCs purchased from LONZA. Ethical approval for the generation of these cells was comprehensively undertaken by LONZA. hMSCs were prepared from human BM (human BM‐MNCs; Lonza, Amagazaki, Japan) as described in our previous report and in the Supplemental Experimental Procedures.³⁰, ³⁵ All experimental protocols were approved by the animal committee of Keio University, Japan. All methods were conducted in strict accordance with the approved guidelines of the institutional animal care committee. LNGFR‐PE (Miltenyi Biotec, Bergisch‐Gladbach, Germany) and THY‐1‐APC (BD Pharmingen, San Jose, California) were added to the human BM cells and incubated for 30 minutes on ice. The tube was centrifuged, the supernatant was discarded, and HBSS containing propidium iodide (Sigma, St. Louis, Missouri) was added. The cells were sorted using a triple‐laser MoFlo (Beckman Coulter, Brea, California), FACS Vantage SE, or FACS Aria III (Becton Dickinson, Bedford, Massachusetts). The data were analyzed using FlowJo software (Tree Star, Ashland, Oregon). All flow cytometry experiments are described in the Supplemental Experimental Procedures.