Crt specific antibody

Manufactured by Abcam

The CRT-specific antibody is a laboratory reagent designed for the detection and analysis of the Calreticulin (CRT) protein. CRT is a calcium-binding protein found in the endoplasmic reticulum of eukaryotic cells and plays a role in protein folding and calcium homeostasis. The CRT-specific antibody can be used in various immunoassay techniques, such as Western blotting, immunohistochemistry, and flow cytometry, to study the expression and localization of CRT in biological samples.

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Lab products found in correlation

Facscalibur cytofluorometer, by BD (2 mentions) Cellquest software, by BD (2 mentions) To pro 3, by Thermo Fisher Scientific (1 mentions) To pro 3, by BD (1 mentions) Isotype matched igg antibodies, by Cell Signaling Technology (1 mentions) Alexa fluor 488 conjugated antibody, by Thermo Fisher Scientific (1 mentions)

2 protocols using crt specific antibody

Quantifying CRT Expression in Cells

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Cells were incubated with a CRT-specific antibody (Abcam) and stained TO-PRO-3 (Life Technologies) and analysed by means of a FACSCalibur cytofluorometer (BD Biosciences) as described in Supplementary Information. First line statistical analyses were performed by using the CellQuest software (BD Biosciences), upon gating on TO-PRO-3 negative events characterized by normal forward and side scatter (living cells).

San José-Enériz E., Agirre X., Rabal O., Vilas-Zornoza A., Sanchez-Arias J.A., Miranda E., Ugarte A., Roa S., Paiva B., Estella-Hermoso de Mendoza A., Alvarez R.M., Casares N., Segura V., Martín-Subero J.I., Ogi F.X., Soule P., Santiveri C.M., Campos-Olivas R., Castellano G., de Barrena M.G., Rodriguez-Madoz J.R., García-Barchino M.J., Lasarte J.J., Avila M.A., Martinez-Climent J.A., Oyarzabal J, & Prosper F. (2017). Discovery of first-in-class reversible dual small molecule inhibitors against G9a and DNMTs in hematological malignancies. Nature Communications, 8, 15424.

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CRT Expression in Living Cells

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Cells were harvested and washed with ice-cold PBS, then incubated with a CRT-specific antibody (Abcam) diluted in cold blocking buffer (5% FBS, v/v in PBS) for 30 min on ice, washed, and incubated with an AlexaFluor^® 488-conjugated antibody (Life Technologies Inc.) in blocking buffer (for 30 min). Thereafter, cells were washed, stained with 1 μg/mL PI in cold PBS for 5 min, and analyzed by means of a FACSCalibur cytofluorometer (BD Biosciences). Isotype-matched IgG antibodies (Cell Signaling Technology) were used as negative staining control. First line statistical analyses were performed by using the CellQuest™ software (BD Biosciences), upon gating on PI^- events characterized by normal forward and side scatter (living cells).

Sukkurwala A.Q., Adjemian S., Senovilla L., Michaud M., Spaggiari S., Vacchelli E., Baracco E.E., Galluzzi L., Zitvogel L., Kepp O, & Kroemer G. (2014). Screening of novel immunogenic cell death inducers within the NCI Mechanistic Diversity Set. Oncoimmunology, 3, e28473.

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