Apc conjugated anti pd l1

Manufactured by BD

Sourced in United States

APC-conjugated anti PD-L1 is a fluorescently-labeled antibody that binds to the PD-L1 protein. PD-L1 is a cell surface protein involved in the regulation of the immune response. The APC fluorescent label allows for detection and analysis of PD-L1 expression on cells using flow cytometry or other fluorescence-based techniques.

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2 protocols using apc conjugated anti pd l1

Multicolor Flow Cytometry Analysis

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Multicolor flow cytometry was performed using the following commercially available antibodies: phycoerythrin (PE)-conjugated anti-human PD-1, allophycocyanin (APC)-cyanine 7 (Cy7)-conjugated anti-mouse F4/80 and CD4 (BioLegend, San Diego, CA, USA), PE-conjugated anti-mouse PD-1, PerCP-Cy5.5-conjugated anti-mouse CD8a (eBioscience, San Diego, CA, USA), V500-conjugated anti-human CD3, V450-conjugated anti-human CD8, APC-H7-conjugated anti-human CD4, APC-conjugated anti-human CD69, V450-conjugated anti-mouse CD3 and CD11b, APC-conjugated anti-mouse CD69, V500-conjugated anti-mouse CD45, APC-conjugated anti PD-L1, and PE-Cy7-conjugated anti-mouse Ly6G (BD Biosciences, San Jose, CA, USA). Dead cells were excluded using the aqua fluorescent LIVE/DEAD dye (Invitrogen). Multicolor flow cytometry was performed using the LSR Fortessa, Canto II instrument (BD Biosciences). Data were analyzed using FlowJo software (TreeStar, Ashland, OR, USA).

Park D.J., Sung P.S., Lee G.W., Cho S.W., Kim S.M., Kang B.Y., Hur W.H., Yang H., Lee S.K., Lee S.H., Jung E.S., Seo C.H., Ahn J., Choi H.J., You Y.K., Jang J.W., Bae S.H., Choi J.Y, & Yoon S.K. (2021). Preferential Expression of Programmed Death Ligand 1 Protein in Tumor-Associated Macrophages and Its Potential Role in Immunotherapy for Hepatocellular Carcinoma. International Journal of Molecular Sciences, 22(9), 4710.

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Comprehensive Stemness and EMT Marker Profiling

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COMMD1 (NBP2-4633, Novusbio), ZEB1 (NBP2-13159, Novusbio), Sox2 (ab97959, Abcam), KLF4 (ab151733, Abcam), Oct-4 (2750, Cell Signaling), MYC (5605, Cell Signaling), NANOG (D73G4)(4903, Cell Signaling), K8/18 (20R-Cp004, Fitzgerald Industries), P53 (SC-126, Santa Cruz Biotechnology), Phospho-NFκB p65 (Ser536) (93H1)(3033, Cell Signaling), HIF1A(610959, BD), HIF2A(NB100-122, Novusbio), MCT4(sc-50329, scbt), CAV1(610407, BD), B-actin (A5441, Sigma), tubulin (T4026, Sigma), FITC-conjugated anti-CD24, PE-conjugated anti-CD24, APC-conjugated anti-CD44, APC-conjugated anti-PD-L1 (BD Biosciences).

Lin Z., Roche M.E., Díaz-Barros V., Domingo-Vidal M., Whitaker-Menezes D., Tuluc M., Uppal G., Caro J., Curry J.M, & Martinez-Outschoorn U. (2024). MiR-200c reprograms fibroblasts to recapitulate the phenotype of CAFs in breast cancer progression. Cell Stress, 8, 1-20.

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