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Cary 500 uv vis nir spectrometer

Manufactured by Agilent Technologies
Sourced in United States

The Cary 500 UV-vis-NIR Spectrometer is a high-performance laboratory instrument designed for measuring the absorption, transmission, and reflectance of samples across the ultraviolet, visible, and near-infrared spectral regions. It provides accurate and reliable data for a wide range of applications.

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5 protocols using cary 500 uv vis nir spectrometer

1

Characterization of Pt@HNB Nanoporous Particles

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X-ray powder diffraction (XRD) data were collected for the host and
protonated hexaniobate compounds on a Philips X’Pert diffractometer
utilizing Cu Kα radiation (λ = 1.5418 Å) and a curved
graphite monochromator at a voltage of 45 kV and a current of 40 mA.
Attenuated total reflection-Fourier transform infrared (ATR-FTIR)
spectroscopy was conducted using a Bruker Alpha II Compact FT-IR spectrometer.
Visible range absorbance spectra were collected using a Cary 500 UV–vis/NIR
spectrometer. For transmission electron microscopy (TEM) observations,
catalysts were dispersed in hexane and drop-cast onto 200-mesh copper
grids. Both standard and high-resolution TEM images were obtained
using an FEI G2 F30 Tecnai Twin transmission electron microscope
at an accelerating voltage of 300 kV. These were used to study catalyst
morphology, size, and Pt loading. TEM images of Pt@HNB NPPs were analyzed
with ImageJ software to find interlayer spacing values of the multiwalled
NPPs, determine inner and outer NPP diameters, and calculate filling
fractions for the NPPs. ImageJ was also used to calculate active Pt
loading by weight percentage for both the Pt@HNB NPPs and extPt-HNB
NScs.
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2

NMR Characterization of Organometallic Compounds

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Fluorine-19 (376 MHz) and 1H (400 MHz) NMR spectra were recorded using a Varian INOVA 400 instrument using a 1 s relaxation time, 60° pulse angle, and 90/10 (v/v) PhCN/C6D6 or CDCl3 as the solvent with a trace amount of C6F6 (δ(19F) –164.90) added as the internal standard. Samples for spectra of 1 or 2 recorded at 23(1) °C were prepared without the exclusion of air; samples for spectra recorded at elevated temperatures and/or with added PS, CoCp2, or Sn2(n-Bu)6 were prepared anaerobically. The program MestReNova 8.1.1 was used to simulate the 19F NMR spectra of 1 and 2. The uncertainties in the fitted J(FF) values are probably ±1 Hz. Mass spectra were recorded using a 2000 Finnigan LCQ-DUO mass-spectrometer with CH3CN used as the carrier solvent. UV-vis spectra of samples dissolved in toluene were recorded using a Cary 500 UV-vis-NIR spectrometer.
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3

Optical Transmittance of Nanomaterials

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The optical transmittance of all samples on glass (Figure 3) were measured in air at normal incidence by a Cary 500 UV-vis-NIR Spectrometer (Varian, Palo Alto, CA, USA), in the 4001000 nm spectral range and normalized to the corresponding signal of a bare glass substrate, with a spectral resolution of 1 nm in the 4001200 nm spectral range.
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4

Transmission Spectra of Au Nanohole Films

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The zero-order transmission spectra of the fabricated NH samples were recorded by using a Cary 500 UV-VIS-NIR Spectrometer (Varian, USA) in air at room temperature under white light incident perpendicularly on the surface of the Au NH films. All transmission spectra were acquired in the spectral range from 400 to 1000 nm with a spectral resolution Δλ = 1 nm and normalized to the transmission spectrum of a bare cleaned glass substrate.
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5

Characterization of Ru(bpy)3Cl2 Sensitized Photoanode

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Triton X-100 and NaClO were purchased from Sigma. Tris(2,2′-bipyridyl)dichlororuthenium(ii)hexahydrate (Ru(bpy)3Cl2·6H2O) was obtained from Aldrich. Tetraethylorthosilicate (TEOS) was bought from Beijing Yili Chemical Reagent. K2HPO4·3H2O and KH2PO4 were used to prepare the phosphate buffer. All of the other chemicals were of analytical grade and were used without further purification. Ultrapure water (>18 MΩ) from Water Purifier (Sichuan Water Purifier Co., Ltd., China) was utilized throughout the experiments. The single-side conductive FTO chips (transmittance > 83%, sheet resistance < 15 Ω sq–1) were purchased from Zhuhai Kaivo Optoelectronic Technology Co., Ltd., China.
The absorption measurements were carried out on a Cary 500 UV-vis-NIR spectrometer (Varian). Photoluminescence measurements were conducted with a Fluoromax-4 spectrofluorometer (Horiba Jobin Yvon Inc., France); the excitation and emission slit widths were 5 nm. Electrochemical experiments were performed on a CHI 660 electrochemical workstation.
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