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2 protocols using anti notch1 c terminal

1

Immunoblotting Analysis of Notch1 and Associated Proteins

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Whole-cell extracts were prepared and then Western blot analysis was performed with anti-Notch1 C-terminal, anti-E-cadherin, anti-plakoglobin, anti-vimentin, anti-p53 (Santa Cruz), anti-N-cadherin (BD Biosciences), anti-cleaved Notch1, anti-FAK (Cell Signaling Technology), anti-pFAK Y397 (Invitrogen), and anti-GAPDH (Biogenesis) antibodies [45 (link)].
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2

Western Blotting of Cellular Proteins

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Whole-cell extracts were prepared and then resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis as described27 (link). Western blot analyses were performed with anti-Notch1 C terminal, anti-Ets-1, anti-DNMT3B (Santa Cruz), anti-cleaved Notch1, anti-ERK1/2, anti-phospho-ERK1/2 (Cell Signaling Technology), anti-TRPA1 (Novus Biologicals), anti-vimentin (Thermo Fisher Scientific), and anti-GAPDH (Genetex) antibodies.
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